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All Journal PELS (Procedia of Engineering and Life Science)
Khaled, Shifaa Waleed
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Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering

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RAPD Markers Distinguish Ground Beetle Species Using Genetic Polymorphism Humadi, Ashwak T.; Sultan, Ammar Ahmed; Khaled, Shifaa Waleed
Procedia of Engineering and Life Science Vol. 5 (2024): Proceedings of the 7th Seminar Nasional Sains 2024
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pels.v7i0.1629

Abstract

This study focuses on differentiating between two ground beetle species, Oryctes rhinoceros and Allomyrina dichotoma, using RAPD markers due to the limitations of phenotypic methods. Ten RAPD primers were employed to identify polymorphic markers. Results showed a total of 41 polymorphic bands, with primers OP-C06 and OP-C15 displaying significant variation in polymorphism percentages, 100% and 16.7% respectively. Primer OP-A08 demonstrated the highest efficiency rate (17.07%) and effectively distinguished between the two beetle species, unlike primers OP-B09, OP-B14, and OP-C10, which showed zero polymorphism. This study underscores the utility of RAPD markers, particularly OP-A08, in accurately identifying insect species, thereby advancing genetic classification techniques. Highlights: Effective Distinction: OP-A08 distinguished Oryctes rhinoceros and Allomyrina dichotoma. High Efficiency: OP-A08 had the highest efficiency rate at 17.07%. Reliable Classification: RAPD markers outperform phenotypic methods for insect identification. Keywords: RAPD Markers, Genetic Polymorphism, Ground Beetles, Insect Classification, Molecular Genetics
Effective Primer Selection for Differentiating Periplaneta Species via PCR Khaled, Shifaa Waleed; Hamad, Sara Salam
Procedia of Engineering and Life Science Vol. 4 (2023): Proceedings of the 6th Seminar Nasional Sains 2023
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/pels.v4i0.1630

Abstract

This study utilized random polymerase chain reaction (PCR) with 10 random primers to differentiate between two insect species, Aegyptica Periplaneta and Periplaneta japonic. The results revealed a total of 29 bands, with 23 being polymorphic. The classification of the animal kingdom, especially insects, is complex due to the abundance and diversity of organisms. Traditional phenotypic methods often fall short in identifying closely related species, necessitating molecular approaches. Primers OP-A04, OP-A08, OP-B14, OP-B18, OP-C10, OP-C15, and OP-C18 showed the highest polymorphism (100%), while OP-A15 and OP-B09 had the lowest (40%). Primer OP-C06 exhibited no bands, indicating 0% polymorphism. Primers OP-A08 and OP-A15 had the highest efficiency (17.241%), whereas OP-C06, OP-C10, and OP-C15 had the lowest (3.448%). Notably, primer OP-A15 successfully discriminated between the species, while OP-C06 failed entirely. These findings underscore the importance of primer selection in molecular identification and suggest OP-A15 as a reliable primer for distinguishing between these Periplaneta species. Highlights: Primer OP-A15 effectively discriminated between Aegyptica Periplaneta and Periplaneta japonic. High polymorphism observed with primers OP-A04, OP-A08, OP-B14, OP-B18, OP-C10, OP-C15, and OP-C18. Primer OP-C06 showed 0% polymorphism and failed to differentiate between species. Keywords: Insect Classification, PCR, Random Primers, Species Differentiation, Periplaneta
Co-Authors Hamad, Sara Salam Humadi, Ashwak T. Sultan, Ammar Ahmed