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All Journal BIOEDUKASI Bioscientist : Jurnal Ilmiah Biologi
Dasmyta, Eny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Library & Information Science Social Sciences

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Identification of Chemical Compounds of Ocimum basilicum (L.) Essential Oil and Cymbopogon nardus (L.) Rendle Essential Oil Using Gas Chromatography- Mass Spectrophotometry (GC-MS) Dasmyta, Eny; Anwar, Yelfi; Maryan, Roni; Muryanto, Muryanto
BIOEDUKASI Vol 22 No. 2 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i2.47800

Abstract

Essential oils are also commonly known as flying oils which can generally be obtain from roots, plant parts, bark, leaves, stems, seed fruits, and flower by steam distillation. The aim of the research is to determine the chemicals components of essential oils of basil (Ocimum basilicum L.) and citronella (Cymbopogon nardus (L.) Rendle) leaves obtained from farmers and processors in Karanganyar, Central Java. Essential oil composition was analysis use gas chromatographys-mass spectrophotometry (GC-MS) method. The finding explain namely chemical component of basil leaf essential oil show that the main component were Estragole (55.80%) and Linalool (22.35%), while in citronella essential oil show that the main component were Geraniol (26.49%), Citronellol (14.53%) and Citronellal (10.95%).
Formulasi Sediaan Sabun Cair Cuci Tangan Kombinasi Minyak Atsiri Daun Kemangi dan Minyak Atsiri Sereh Wangi serta Uji Aktivitas Antibakteri Dasmyta, Eny; Anwar, Yelfi; Maryana, Roni; Muryanto, Muryanto
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12512

Abstract

Liquid hand soap is a cleaning product specifically designed to clean hands from dirt, oil, and microorganisms. The study aims to provide evidence of antibacterial efficacy with a combination of basil leaf essential oil and citronella essential oil on Escherichia coli and Staphylococcus aureus bacteria in hand washing liquid soap formulations. The type of research used is experimental. Essential oils were analyzed by GC-MS and then through formulation as hand washing liquid soap with each concentration of 1%, 1.5%, and 2%. The test results were evaluated, and analyzed using Two Way ANOVA and Post Hoc Tukey.The results of this study showed that the GC-MS test on basil leaf essential oil showed 2 main compounds were detected, namely Estragole (55.80%) and Linalool (22.35%), while in citronella essential oil 3 main compounds were detected, namely Geraniol (26.49%), Citronellol (14.53%), and Citronellal (10.95%). And the results of antibacterial activity tests against Escherichia coli bacteria show each inhibition zone FI 9.32 mm, FII 10.72 mm, and FIII 14.45 mm. While against Staphylococcus aureus bacteria showed each inhibition zone FI 8.75 mm, FII 10.42 mm, and FIII 13.57 mm. For stability tests with temperature (2-8oC) and room temperature (20-25oC), the hand washing liquid soap formulation does not show homogeneous changes, organoleptic preparations, has a pH range of 8.87 - 9.04, has a viscosity consistency that has met the standards between 400-4000 cPs, foam height that meets the standards between 13-220 mm and does not cause irritation to the hands. In brief, the formulation is stable in both temperature (2-8oC) and room temperature (20-25oC) with strong inhibition at 2% concentration.
Formulasi Sediaan Sabun Cair Cuci Tangan Kombinasi Minyak Atsiri Daun Kemangi dan Minyak Atsiri Sereh Wangi serta Uji Aktivitas Antibakteri Dasmyta, Eny; Anwar, Yelfi; Maryana, Roni; Muryanto, Muryanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12512

Abstract

Liquid hand soap is a cleaning product specifically designed to clean hands from dirt, oil, and microorganisms. The study aims to provide evidence of antibacterial efficacy with a combination of basil leaf essential oil and citronella essential oil on Escherichia coli and Staphylococcus aureus bacteria in hand washing liquid soap formulations. The type of research used is experimental. Essential oils were analyzed by GC-MS and then through formulation as hand washing liquid soap with each concentration of 1%, 1.5%, and 2%. The test results were evaluated, and analyzed using Two Way ANOVA and Post Hoc Tukey.The results of this study showed that the GC-MS test on basil leaf essential oil showed 2 main compounds were detected, namely Estragole (55.80%) and Linalool (22.35%), while in citronella essential oil 3 main compounds were detected, namely Geraniol (26.49%), Citronellol (14.53%), and Citronellal (10.95%). And the results of antibacterial activity tests against Escherichia coli bacteria show each inhibition zone FI 9.32 mm, FII 10.72 mm, and FIII 14.45 mm. While against Staphylococcus aureus bacteria showed each inhibition zone FI 8.75 mm, FII 10.42 mm, and FIII 13.57 mm. For stability tests with temperature (2-8oC) and room temperature (20-25oC), the hand washing liquid soap formulation does not show homogeneous changes, organoleptic preparations, has a pH range of 8.87 - 9.04, has a viscosity consistency that has met the standards between 400-4000 cPs, foam height that meets the standards between 13-220 mm and does not cause irritation to the hands. In brief, the formulation is stable in both temperature (2-8oC) and room temperature (20-25oC) with strong inhibition at 2% concentration.
Co-Authors Anwar, Yelfi Maryan, Roni Maryana, Roni Muryanto Muryanto, Muryanto