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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of Engineering, Electrical and Informatics
Miftahudin , Miftahudin
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Agriculture, Biological Sciences & Forestry Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Engineering Environmental Science

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Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Rice Cultivars: Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Sindiya, Vita; Fendiyanto, Miftahul Huda; Hamim, Hamim; Miftahudin , Miftahudin
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.11

Abstract

Rice cv. Hawara Bunar is a local rice cultivar tolerant to aluminum (Al) and drought stress. However, the cultivar has inferior characteristics, such as a tall habitus and a small number of tillers, making the cultivar agronomically unattractive. Many genes control plant height and tiller number; one is the TAD1 gene. Analysis of gene expression in two contrasting rice cultivars for both characters is a prerequisite for selecting certain genes for gene editing targets. This study aimed to analyze the gene expression of the TAD1 in rice cv. IR64 and Hawara Bunar, and to construct a phylogenetic tree of genes that regulate rice plant height and tiller number. Gene expression analysis was conducted using the qRT-PCR technique, while the phylogenetic tree was constructed based on the Neighbor-Joining method using PAUP4 software. The results showed TAD1 gene expression in the tillering phase of rice cv. Hawara Bunar is higher than the cv. IR64. The gene expression level in both cultivars corresponds to the plant height and tiller number characters in both rice cultivars. Phylogenetic analysis showed that the TAD1 gene clustered with genes that cause the rice to have a tall habitus with few tillers. The results of a transcriptome meta-analysis reinforced the phylogenetic tree, which shows that the TAD1 gene was found in a group of downregulated genes based on the volcano plots. Therefore, the TAD1 gene can be selected as a target gene for editing in rice cv. Hawara Bunar to obtain superior characters.
Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Rice Cultivars: Expression of TAD1 (Tillering & Dwarf1) Gene in Hawara Bunar and IR64 Sindiya, Vita; Fendiyanto, Miftahul Huda; Hamim, Hamim; Miftahudin , Miftahudin
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.11

Abstract

Rice cv. Hawara Bunar is a local rice cultivar tolerant to aluminum (Al) and drought stress. However, the cultivar has inferior characteristics, such as a tall habitus and a small number of tillers, making the cultivar agronomically unattractive. Many genes control plant height and tiller number; one is the TAD1 gene. Analysis of gene expression in two contrasting rice cultivars for both characters is a prerequisite for selecting certain genes for gene editing targets. This study aimed to analyze the gene expression of the TAD1 in rice cv. IR64 and Hawara Bunar, and to construct a phylogenetic tree of genes that regulate rice plant height and tiller number. Gene expression analysis was conducted using the qRT-PCR technique, while the phylogenetic tree was constructed based on the Neighbor-Joining method using PAUP4 software. The results showed TAD1 gene expression in the tillering phase of rice cv. Hawara Bunar is higher than the cv. IR64. The gene expression level in both cultivars corresponds to the plant height and tiller number characters in both rice cultivars. Phylogenetic analysis showed that the TAD1 gene clustered with genes that cause the rice to have a tall habitus with few tillers. The results of a transcriptome meta-analysis reinforced the phylogenetic tree, which shows that the TAD1 gene was found in a group of downregulated genes based on the volcano plots. Therefore, the TAD1 gene can be selected as a target gene for editing in rice cv. Hawara Bunar to obtain superior characters.
The Phenomenon of Waterlogging Due to Poor Drainage in the Tanjung Duren Area, West Jakarta Miftahudin , Miftahudin; Marsya Luna Lestari; Tri Susanto; Salsabila Ahadini Nurahmat; Williamzu Williamzu; Darmawan Listya Cahya
Journal of Engineering, Electrical and Informatics Vol. 5 No. 3 (2025): October: Journal of Engineering, Electrical and Informatics
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jeei.v5i3.5659

Abstract

Tanjung Duren in West Jakarta is a dense urban area with a high level of development and activity, which has an impact on increasing waterproofing surfaces and decreasing drainage capacity. This research aims to identify the main causes of inundation, evaluate the performance of drainage systems, and formulate ap-plicable technical and non-technical solutions. A mixed-method approach was used, including field ob-servations at inundation points, technical measurements of channels, citizen perception surveys, and semi-structured interviews. The results showed that clogging by garbage, inappropriate channel cross-sections, and poor drainage connectivity were the main causes of inundation. The impact of in-undation includes mobility disruptions, economic losses, and health risks to residents. Hydrologi-cal-hydraulic analysis showed a mismatch between runoff discharge (Qin) and channel capacity (Qcap), with a ratio value of ρ ≥ 1 at some points. The proposed solutions consist of routine cleaning, increasing channel capacity, implementing infiltration wells, and strengthening community participation. Recom-mendations also include the establishment of a local taskforce and a waste management education pro-gram. This research emphasizes the importance of an integrated approach based on data and cross-sectoral collaboration in sustainable urban drainage management.
Co-Authors Darmawan Listya Cahya Fendiyanto, Miftahul Huda Hamim Hamim Marsya Luna Lestari Salsabila Ahadini Nurahmat Sindiya, Vita Tri Susanto Williamzu Williamzu