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All Journal Jurnal Analis Medika Biosains (JAMBS) JURNAL ANALIS LABORATORIUM MEDIK Abdimas Indonesian Journal
Zafrida, Siska
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Religion Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences

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Hypertension Education as An Effort to Increase Knowledge and Prevent Hypertension in The Elderly at Posyandu Mawar Merah, Labuh Baru Barat Sub-District, Payung Sekaki District, Pekanbaru Zafrida, Siska; Budiman, Alfi; Akram, Faiz Jamal; Hamdani, Salsabila Putri
Abdimas Indonesian Journal Vol. 4 No. 1 (2024)
Publisher : Civiliza Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59525/aij.v4i1.340

Abstract

Hypertension is a non-communicable disease that can attack the human body, whether it is a woman or a woman. The proportion of the elderly population in Indonesia in 2025 will increase to approximately 11.8% of the total population. Elderly health is one of the government's programs. The public health problem facing the elderly currently is the increasing number of cases of non-communicable diseases (NCDs), one of which is hypertension. Most of the elderly who live and are assisted in the Madania elderly social home suffer from hypertension. In this institution there is no blood pressure monitoring program and education about hypertension. This activity aims to understand the blood pressure picture of the elderly and increase the knowledge of the elderly about hypertension, so that they can carry out prevention and treatment independently. Activities were carried out in June 2023, in the form of education about hypertension to the elderly and measuring blood pressure. The method used is lecture and discussion using leaflet media. The activity was attended by 38 elderly people. Participants looked enthusiastic in participating in the entire activity agenda. There is an increase in knowledge after being given counseling material.
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE DARI USUS IKAN SELAIIS (KRYPTOPTERUS LAIS) Zafrida, Siska; Yulianto, Ignatius; Riana, Alberta Ida; Gusrinaldi; Siahaan, Lelli Preti
Jurnal Analis Laboratorium Medik Vol 9 No 2 (2024): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v9i2.5346

Abstract

Penggunaan enzim di Indonesia sangat tinggi karena enzim banyak digunakan di berbagai bidang industri dan kesehatan. Pemanfaatan enzim protease dalam bidang kesehatan yaitu untuk pengobatan radang, tumor, pengatur kekebalan darah dan kelainan darah. Saluran pencernaan ikan berpotensi sebagai sumber enzim protease karena pada isi perut ikan terdapat organ pencernaan yang berfungsi sebagai sistem metabolisme tempat protein terhidrolisis yang mengandung banyak protease. Salah satu mikroorganisme penghasil enzim protease adalah bakteri proteolitik yaitu bakteri yang mampu mendegradasi protein dan menghasilkan protease ekstraseluler. Penelitian ini bertujuan mendapatkan isolat bakteri penghasil protease pada saluran pencernaan ikan selais  yaitu pada usus dan lambung ikan selais. Isolat bakteri yang diperoleh diuji tingkat patogenitas dengan media Mac Conkey (MC) dan Blood Agar Plate (BAP). Uji produksi enzim protease dilakukan pada media Skim Milk Agar yang ditandai dengan pembentukan zona bening. Dari hasil penelitian ini diperoleh 4 isolat bakteri, terdapat 1 isolat (KLI 1) diantaranya  merupakan bakteri penghasil protease berdasarkan hasil 16s rRNA yaitu Proteus mirabillis. Isolat bakteri yang dapat menghasilkan enzim protease tersebut di diharapkan dapat digunakan dalam industri kesehatan yaitu pada penggunaan bahan untuk pengobatan dan kelainan darah.
UJI AKTIVITAS ANTELMINTIK EKSTRAK DAUN MYANA (COLEUS ATROPURPUREUS) TERHADAP TELUR SOIL TRANSMITTED HELMINTH Hartuti, Yeli; Zafrida, Siska
Jurnal Analis Laboratorium Medik Vol 9 No 2 (2024): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v9i2.5347

Abstract

Infeksi akibat cacing (Helminthiasis) merupakan salah satu infeksi yang sering menyerang anak-anak dengan tingkat ekonomi yang rendah terutama pada Negara berkembang. Myana merupakan tanaman hias yang dapat dimanfaatkan sebagai obat tradisional. Daun myana diketahui memiliki senyawa metabolit sekunder yang berpotensi sebagai antelmintik. Daun myana berkhasiat untuk penetrasi racun, menghambat pertumbuhan bakteri, mempercepat pematangan bisul dan pembunuh cacing. Penelitian ini bertujuan untuk mengetahui senyawa metabolit sekunder yang terkandung dalam daun myana, mengetahui aktivitas antelmintik ekstrak etanol daun myana terhadap telur cacing Soil Transmitted Helminth serta mengetahui konsentrasi ekstrak daun myana yang menunjukkan aktivitas antelmintik. Uji Aktivitas Antelmintik ekstrak etanol daun myana terhadap telur Ascaris lumbricoides, Trichuris trichiura, Hookworm dibagi kedalam dua kelompok, yaitu kelompok pembanding (Pirantel pamoat) dan kelompok uji (Ekstrak etanol daun myana konsentrasi 20%,40%,60%,80%,100%). Pengujian aktivitas antelmintik dilakukan dengan melihat efek yang ditimbulkan oleh kelompok uji berupa perubahan morfologi telur cacing (lisis/tidak lisis) serta perubahan warna pada telur cacing. Hasil penelitian diketahui senyawa metabolit sekunder yang terkandung dalam ekstrak daun myana yaitu flavonoid, tanin, safonin dan steroid. Ekstrak etanol daun myana memiliki aktivitas antelmintik terhadap telur Ascaris lumbricoides pada konsentrasi 60%,80% dan 100%. Aktivitas tersebut meningkat seiring dengan peningkatan konsentrasi ekstrak sedangkan ekstrak etanol daun myana tidak memiliki aktivitas antelmintik terhadap telur Trichuris trichiura dan Hookworm.
Overview of Platelet Counts Before and After Platelet-Rich Plasma (PRP) Preparation Yuliandari, Aisyara; Mahrani, Izah; Zafrida, Siska; Hartini , Hartini; Fauzan, Fauzan
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

Platelet-Rich Plasma (PRP) is widely utilized in various clinical settings for tissue repair, particularly in skin rejuvenation, scar management, and wound healing. This study aimed to evaluate the profile of platelet counts both prior to and following the PRP preparation process. Whole blood samples were collected from eligible female participants and platelet levels analyzed using a hematology analyzer. The findings revealed a four-fold rise in the mean platelet count, increasing from a baseline of 190.6×103 cells/µL to 781.1×103 cells/μL after preparation. The study concluded that baseline platelet levels in whole blood are directly proportional to the final platelet concentration in PRP.
Isolation and Screening of Proteolytic Bacteria Isolated from the Digestive Tract of Pangasius Catfish Patin (Pangasius hypophthalmus) Zafrida, Siska; Lasmini, Titi; Hartini, Hartini; Aruan, Anggi Agustina
Jurnal Analis Medika Biosains (JAMBS) Vol. 13 No. 01 (2026): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

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Abstract

The demand for protease enzymes in Indonesia has exhibited a significant upward trend; however, domestic large-scale production capacity remains limited. To mitigate dependency on imports, strategic efforts to develop indigenous protease production are essential. This study aims to identify and isolate protease-producing bacteria from the digestive tract of catfish (Pangasius hypophthalmus). This research employed an observational method with a cross-sectional design. Samples were obtained from the stomach of the catfish and subsequently inoculated into Brain Heart Infusion (BHI) media. Bacterial isolation was performed using Blood Agar Plate (BAP) and MacConkey Agar. The proteolytic activity of the resulting isolates was evaluated on Skim Milk Agar (SMA), followed by macroscopic and microscopic characterization. The study yielded three pure isolates; however, screening for proteolytic activity revealed that only one isolate, designated as PHS1, exhibited protease production. The proteolytic activity was characterized by the formation of a 2 mm clear zone (lysis zone) with a proteolytic index of 3. Microscopic analysis identified the isolate as a Gram-positive, spore-forming bacterium.
Co-Authors Aisyara Yuliandari Akram, Faiz Jamal Aruan, Anggi Agustina Budiman, Alfi Fauzan . Gusrinaldi Hamdani, Salsabila Putri Hartini Hartini , Hartini Hartuti, Yeli Mahrani, Izah Riana, Alberta Ida Siahaan, Lelli Preti Titi Lasmini Yulianto, Ignatius