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All Journal Jurnal Ilmu Ternak Veteriner Hasanuddin Journal of Animal Science (HAJAS)
Fatmyah Utamy, Renny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Immunology & microbiology Veterinary

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Effect of Bali cattle ovarian status on oocytes nuclear maturation and in vitro fertilization rate Herry Sonjaya; M. Yusuf; A. Hamdana; Renny Fatmyah Utamy; Sri Gustina; Hasbi Hasbi
Jurnal Ilmu Ternak dan Veteriner Vol 22, No 4 (2017): DECEMBER 2017
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.074 KB) | DOI: 10.14334/jitv.v22i4.1585

Abstract

The aim of this study was to investigate whether the reproductive status influences the nuclear maturation and fertilization rates of bali cattle oocytes in vitro. Several pairs of ovary were classified into four groups: 1) ovaries with Corpus Luteum (CL) and Dominant Follicle (DF), 2) ovaries without CL and with DF, 3) ovaries with CL and without DF, 4) ovaries without both CL and DF. In the first experiment, oocytes were collected by slicing method in Phosphate Buffer Saline (PBS) medium supplemented with 10% Fetal Bovine Serum (FBS) and 100 IU/ml penicillin streptomycin. Oocytes were matured in tissue culture medium (TCM)-199 supplemented with 10% Fetal Bovine Serum (FBS), 10 IU/ml Follicle Stimulating Hormone (FSH), 10 IU/ml Luteinizing Hormone (LH), and 50 μg/ml gentamycin. Oocytes were matured in 5% CO2 incubator, 38oC for 24 h. In the second experiment, oocytes were matured and then fertilized in vitro to observe pronuclear formation. The first experiment showed that the percentage of oocytes reached methaphase-II (MII) stage on ovaries with CL and without DF (89.47%) were higher (P<0,01) compared to ovaries without both CL and DF (75,47%), ovaries without CL and with DF (74.,41%), or ovaries with CL and DF (65,52%). The result of second experiment showed that the ovarian reproductive status was not significantly different (P>0.05) on fertilization rate.
Effect of substituting concentrate with dwarf Napier grass (Pennisetum purpureum) on intake, growth and carcass composition of rabbits Mohammad Mijanur Rahman; Mastura Nazirah Binti Mustapher; Khairiyah Binti Mat; Renny Fatmyah Utamy; Maryana Mohamad Nor
Hasanuddin Journal of Animal Science (HAJAS) Vol. 2, No. 1 (2020)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/hajas.viNo 1.9853

Abstract

Dwarf Napier grass (Pennisetum purpureum) is considered to be more suitable as forage for ruminants due to its high yield and nutritive value, but there is limited research on rabbits. Thus, the aim of this study was to investigate the effect of replacing concentrate with dwarf Napier grass on intake, gorwth performance and carcass composition of growing rabbits. Twelve growing rabbits were randomly distributed into three dietary groups in a completely randomised design: (i) concentrate feed ad libitum as control diet (T1), (ii) half of the control diet plus dwarf Napier grass ad libitum (T2), and (iii) quarter of the control diet plus dwarf Napier grass ad libitum (T3). The results showed that diets had a significant (p<0.05) effect on intake, growth performance and some non-carcass components. Rabbits fed T3 diet showed significantly (p<0.05) lower total DM intake than those fed T1 and T2 diets. Similarly, rabbits fed T3 diet showed significantly (p<0.05) lower total weight gain and daily weight gain than those fed T1 diet, but the respective values of those fed T2 diet were non-signifiacntly different from those fed T1 and T3 diets. There was significant effect on weights of meat with bone, fat, pelt, head and kidney by the diets, whereas weights of most of the non-carcass components were similar among the groups. In conclusion, diet consisting of half of the concentrate and dwarf Napier grass ad libitum is recommended to be used as it may reduce the feed cost compared to diet consisting of the concentrate alone.
Morphology Traits and Dry Matter Yield of Pennisetum purpureum Pakchong 1 Fertilized by Liquid Organic Fertilizer Kalebbi, Muzakkir; Nohong, Budiman; Fatmyah Utamy, Renny
Hasanuddin Journal of Animal Science (HAJAS) Vol. 6 No. 1 (2024)
Publisher : Faculty of Animal Science, Hasanuddin University, Makassar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Forage must be available sustainably in both quality and quantity to support ruminant farming, so it requires soil that contains sufficient nutrients. This study aims to determine the growth, production, and quality of Pakchong elephant grass after being given liquid organic fertilizer in different doses. The experiment conducted in this study used a completely randomized design (CRD) with five treatments and five replications, namely P0 (No liquid organic fertilizer), P1 (Liquid organic fertilizer 40 liters/ha equivalent to 29 ml/plot), P2 (Liquid organic fertilizer 50 liters/ha equivalent to 36 ml/plot), P3 (Liquid organic fertilizer 60 liters/ha equivalent to 43 ml/plot), P4 (Liquid organic fertilizer 70 liters/ha equivalent to 50 ml/plot). The results showed that implementing various levels of liquid organic fertilizer positively impacted the growth of Pakchong elephant grass. In the parameters of plant growth, the best results were obtained in the P4 (70 l/ha) and P3 (60 l/ha) treatments compared to P0 (without fertilizer). Production of Pakchong elephant grass using liquid organic fertilizer in different doses obtained the best results on treatments P4 and P3. The provision of different doses did not significantly affect (p>0.01)the nutritional content of   Pakchong grass. Keywords: Organic liquid fertilizer, Pakchong, growth, production, quality
Co-Authors A. Hamdana Budiman Nohong, Budiman H. Hasbi Herry Sonjaya Kalebbi, Muzakkir Khairiyah Binti Mat M. Yusuf Maryana Mohamad Nor Mastura Nazirah Binti Mustapher Mohammad Mijanur Rahman Sri Gustina