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All Journal Biogenesis: Jurnal Ilmiah Biologi Biosaintifika: Journal of Biology & Biology Education
Askitosari, Theresia Desy
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Assessing plant growth and infection in Avicennia marina (Forssk.) Vierh following inoculation with bacterial endophytes Savitri, Wina Dian; Askitosari, Theresia Desy
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 1 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i1.41646

Abstract

Endophytic bacteria play an important role for plants in assisting the efficient absorption of nutrients from the surrounding environment. Studies on endophytic bacteria in Avicennia marina plants are still relatively rare. This research aims to determine abilities of three bacterial endophytes and a control Bacillus subtilis infecting the host plant A. marina and conducted in ekowisata mangrove Gunung Anyar, Surabaya. Each endophyte isolates at the concentration of 102, 103, and 104 CFU/mL were used for inoculation, whereas each concentration was applied to 3 plants (triplicate). Every ± 1 m tall A. marina plant was injected with 2 × 500 µL of bacterial solution. Another three plants were chosen and injected with Lactose-Broth (LB) sterile medium. The leaf length and width of 2-4 leaves per plant were measured at day 0 before inoculation and after two weeks of inoculation. Data analysis using paired t-test resulted in no change in leaf area average before and after treatments. Despite the result, we observed that some of the treated plants produced flowers, whereas no flower was observed on control plants. In addition, there were infection zones detected around the infection area. Furthermore, we have observed Indole Acetic Acid (IAA) and siderophore production in all bacterial isolates. The results of this study show that acceleration in the development of A. marina, evidenced by a phase shift from vegetative to generative occurring within a two-week timeframe.
Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves Hardjo, Popy Hartatie; Askitosari, Theresia Desy; Wijaya, Agnes Natalia; Edbert, Joshua; Artadana, Ida Bagus Made; Wittayathanarattana, Takon
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12057

Abstract

Acehnese Patchouli var. Sidikalang can produce patchouli oil, one of the country's sources of foreign exchange, and it is advantageous in many industries. However, patchouli alcohol (PA) content in patchouli oil is still low. The higher PA content determines the better quality of patchouli oil. So, delignification with Trichoderma viride was carried out to increase PA and yield of patchouli oil. The duration of delignification patchouli leaves is one factor affecting the increase in PA and yield. This study aimed to determine the effect of delignification duration on PA content and yield. The research method was completely randomized with 8 treatments (a combination of 2 treatments: with and without delignification, and 4 delignification durations: 0, 3, 6, and 9 days). The inoculum used for delignification was an inoculum ball, and then patchouli leaf samples were extracted using microwave-assisted extraction (MAE). Gas chromatography was carried out for PA analysis. Delignification of patchouli leaves, with a delignification time of 9 days, showed high PA and yield, which were (0.3129 ± 0.1557)% and (1.4543 ± 0.7717)%, respectively. The success of delignification at  9 days was also shown by increased phenolic content. Trichoderma viride is often utilized in delignification process, but the optimal duration of its delignification of Aceh patchouli leaves that can help improve patchouli oil quality is not yet known. This research is hoped to help related industries improve the secondary metabolites of plants, which has many benefits for the community. Implication/benefit for science development/society.
Co-Authors Artadana, Ida Bagus Made Edbert, Joshua Popy Hartatie Hardjo Wijaya, Agnes Natalia Wina Dian Savitri, Wina Dian Wittayathanarattana, Takon