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All Journal Vegetalika Jurnal Agrotek Tropika AGROMIX Jurnal Pengabdian Masyarakat untuk Negeri (UN-PENMAS)
Popy Hartatie Hardjo
Lab. Bioteknologi Fak. Farmasi Univ. Surabaya
Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Social Sciences Other

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Optimasi Regenerasi Kalus Embriogenik Porang (Amorphophallus muelleri Blume) Melalui Kultur Suspensi Sel Menggunakan BAP (Benzyl Amino Purin) Restanto, Didik Pudji; Prayoga, Mohammad Candra; Arsyika, Izna; Farlisa, Veronenci Yuliarbi; Hardjo, Popy Hartatie
Vegetalika Vol 13, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.100708

Abstract

Porang Amorphophallus muelleri Blume merupakan tanaman umbi-umbian yang banyak dimanfaatkan sebagai bahan kosmetik dan diekspor untuk bahan baku industri. Perbanyakan porang secara konvensional mengalami masa dormansi. Perbanyakan porang melalui kultur jaringan dapat menghasilkan bibit yang cepat dan dalam jumlah yang banyak. Kultur jaringan porang dapat melalui somatik embriogenesis melalui pembentukan kalus dan poliferasi kalus dengan kultur suspensi sel. Optimasi regenerasi kalus hasil poliferasi kultur suspensi sel sangat penting untuk menunjang keberhasilan perbanyakan porang secara masal. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi BAP terhadap regenerasi kalus embriogenik porang Amorphophallus muelleri B. hasil kultur suspensi. Penelitian menggunakan rancangan acak lengkap dengan faktor konsentrasi BAP yang terdiri dari 4 taraf perlakuan antara lain 1 mg.L-1, 2 mg.L-1, 3 mg.L-1, dan 4 mg.L-1. Setiap perlakuan diulang sebanyak 5 ulangan. Analisis data penelitian menggunakan ANOVA dan uji lanjut dengan DMRT pada taraf kesalahan 5%. Analisis data menggunakan aplikasi SPSS statistics versi 26. Berdasarkan hasil penelitian, perlakuan BAP 1 mg.L-1menunjukkan hasil terbaik dengan parameter kedinian munculnya tunas tercepat 45 hst, tunas terpanjang 7.6 cm, jumlah tunas terbanyak 7,33 tunas, dan persentase regenerasi 93,33%. Berdasarkan pengamatan histologi, kalus embriogenik berkembang menghasilkan mature embrio yang lebih banyak sehingga dapat menginduksi tunas yang banyak.
Empowering the Putri Keong Community Community through hydroponics of pakchoy mustard greens (Brassica rapa L.) and processed products Hardjo, Popy Hartatie
UN PENMAS (Jurnal Pengabdian Masyarakat untuk Negeri) Vol 4 No 2 (2024): UN PENMAS Vol 4 No 2
Publisher : LPPM Universitas Narotama

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Abstract

ABSTRACT Community service activities for Pokmas Putri Keong partners aim to increase the knowledge and skills of partners in developing business results in the field of hydroponic cultivation of pakcoy mustard greens and post-harvest handling of pakcoy mustard greens in both fresh and processed products. Based on the survey and situation analysis, the problem was identified as limited understanding and knowledge of hydroponic vegetable cultivation so that harvested products are less productive and only sold fresh directly to consumers and there is no proper post-harvest handling so they cannot be stored, therefore they will be developed into processed products. Community service programs include training in hydroponic and post-harvest cultivation and product processing, mentoring and provision of post-harvest equipment which can increase partner empowerment so that business results can be increased which can then grow even bigger by selling various processed products from harvests cultivated by the partners themselves. Key words: post-harvest, vegetable hydroponics
Effect of Light Color and Auxin on Callus Induction and Development in Amorphophallus muelleri Blume. Hardjo, Popy Hartatie; Thongiratama, Michael Anthony; Irawati, Fenny; Restanto, Didik Pudji
Vegetalika Vol 14, No 3 (2025): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106227

Abstract

Porang (Amorphophallus muelleri Blume) is a significant player in the international market, but its slow seedling growth rate is a major concern. This study, which holds significant implications for porang cultivation, aimed to accelerate the seedling growth phase through the induction of adventitious shoots from callus proliferation. We explored the use of plant growth regulators (PGRs), specifically auxins (NAA and 2,4-D) and cytokinins (BAP), in combination with variations in light color (white and white-blue). The observed parameters included the time of first callus and adventitious shoot emergence, callus growth, number of adventitious shoots, and callus coloration. Data were subjected to two-way analysis of variance (ANOVA) to evaluate the effects of plant growth regulator (PGR) type and light quality as independent variables. Statistical significance was assessed at a 95% confidence level using SPSS software. The combination of 1.0 ppm NAA and 0.5 ppm BAP in MS medium under white-blue light effectively accelerated callus induction from bulbil explants, with visible callus formation by day 8 and the fastest proliferation over 7 weeks. Under white light, the same PGR combination also promoted the earliest adventitious shoot formation by day 16 and yielded the highest number of shoots, totaling seven by the end of the culture period.
The effectiveness of growth regulators and light color spectrum on callus growth of Amorphophallus muelleri Blume. var. Madiun1: Efektivitas zat pengatur tumbuh dan spektrum warna cahaya terhadap pertumbuhan kalus Amorphophallus muelleri Blume. var. Madiun1 Wijaya, Agnes Natalia; Poernomo, Carlois; Savitri, Wina Dian; Irawati, Fenny; Hardjo, Popy Hartatie
AGROMIX Vol 14 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v14i2.3309

Abstract

Introduction: Plant Growth Regulators (PGRs) play a role in regulating organogenesis and morphogenesis in shoots, roots, and callus formation. Color spectrum of light is one of the quality light factors that affects plant physiological processes. This study aimed to determine the effect of cytokinin and auxin on Murashige-Skoog (MS) medium and light on callus induction and proliferation of porang (Amorphophallus muelleri Blume.) var. Madiun1. Methods: This study used completely randomized design, with the first factor was PGRs (combination auxin and cytokinin) and the second was color spectrum of light (white light, blue light, and its combination) during incubation. Variables observed were emergence time, color, texture, structure and calli growth, also shoots emerging from calli. Results: The results showed an interaction between PGRs with a combination of light color spectrum on callus growth. The fastest callus growth occurred in combination 5.0 mg.l-1 6-benzyl amino purine (BAP) with 0.2 mg.l-1 naphthalene acetic acid (NAA) which was incubated in a combination of white and blue light for 16 hour irradiation. The combination 5.0 mg.l-1 BAP with 0.2 mg.l-1 NAA was able to induce callus emergence time, and the shoots appearing were faster, whereas combination of white and blue light was able to accelerate callus emergence from bulbil and adventitious shoots emergence. Conclusion: The combination of white and blue light color spectrum for 16 hours irradiation can accelerate callus emergence from bulbil and adventitious shoots emerging from calli, and interaction with combination of 5.0 mg.l-1 BAP and 0.2 mg.l-1 NAA can accelerate porang’s callus growth.
EFFECT OF NUTRIENT SOLUTION CONCENTRATION ON THE GROWTH PERFORMANCE OF PAKCOY MUSTARD (Brassica rapa L.) UNDER A SENSOR-BASED NUTRIENT FILM TECHNIQUE HYDROPONIC SYSTEM Hardjo, Popy Hartatie; dos Santos, Zelia Camoes; Sugiarno, Stefan Saputra; Plessers, Timothy Amadeus Filhend
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.10465

Abstract

The growth of pakcoy mustard (Brassica rapa L.) is strongly influenced by the concentration and composition of hydroponic nutrient solutions. This study aims to determine the optimal nutrient solution concentration for maximized pakcoy mustard growth at harvest. The experimental setup adopted was a randomized block design featuring 5 different levels of electrical conductivity (EC), specifically 0.8 mS.cm-1, 1.2 mS.cm-1, 1.6 mS.cm-1, 2.0 mS.cm-1, and 2.4 mS.cm-1, with replicated ten times. The parameters evaluated to assess the efficacy of each treatment include plant height, number of leaves, leaf area, overall fresh weight of the plant, and chlorophyll levels. Data were analyzed using Duncan Multiple Range Test (DMRT) at a 5% significance level(p < 0.05). The results indicated that an EC of 2.0 mS.cm-1 led to the greatest growth of pakcoy mustard, achieving a plant height of 9.6±2.38 cm, a leaf count of 23.9±2.13, a leaf area of 32.81±8.95 cm2, and a chlorophyll concentration of 5.9±0.52 mg.l-1with a harvest period of four weeks.
Co-Authors Arsyika, Izna dos Santos, Zelia Camoes Farlisa, Veronenci Yuliarbi Fenny Irawati Plessers, Timothy Amadeus Filhend Poernomo, Carlois Prayoga, Mohammad Candra Restanto, Didik Pudji Sugiarno, Stefan Saputra Thongiratama, Michael Anthony Wijaya, Agnes Natalia Wina Dian Savitri, Wina Dian