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All Journal Vegetalika AGROMIX Jurnal Pengabdian Masyarakat untuk Negeri (UN-PENMAS) Biosaintifika: Journal of Biology & Biology Education
Popy Hartatie Hardjo
Lab. Bioteknologi Fak. Farmasi Univ. Surabaya
Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Social Sciences Other

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Optimasi Regenerasi Kalus Embriogenik Porang (Amorphophallus muelleri Blume) Melalui Kultur Suspensi Sel Menggunakan BAP (Benzyl Amino Purin) Restanto, Didik Pudji; Prayoga, Mohammad Candra; Arsyika, Izna; Farlisa, Veronenci Yuliarbi; Hardjo, Popy Hartatie
Vegetalika Vol 13, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.100708

Abstract

Porang Amorphophallus muelleri Blume merupakan tanaman umbi-umbian yang banyak dimanfaatkan sebagai bahan kosmetik dan diekspor untuk bahan baku industri. Perbanyakan porang secara konvensional mengalami masa dormansi. Perbanyakan porang melalui kultur jaringan dapat menghasilkan bibit yang cepat dan dalam jumlah yang banyak. Kultur jaringan porang dapat melalui somatik embriogenesis melalui pembentukan kalus dan poliferasi kalus dengan kultur suspensi sel. Optimasi regenerasi kalus hasil poliferasi kultur suspensi sel sangat penting untuk menunjang keberhasilan perbanyakan porang secara masal. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi BAP terhadap regenerasi kalus embriogenik porang Amorphophallus muelleri B. hasil kultur suspensi. Penelitian menggunakan rancangan acak lengkap dengan faktor konsentrasi BAP yang terdiri dari 4 taraf perlakuan antara lain 1 mg.L-1, 2 mg.L-1, 3 mg.L-1, dan 4 mg.L-1. Setiap perlakuan diulang sebanyak 5 ulangan. Analisis data penelitian menggunakan ANOVA dan uji lanjut dengan DMRT pada taraf kesalahan 5%. Analisis data menggunakan aplikasi SPSS statistics versi 26. Berdasarkan hasil penelitian, perlakuan BAP 1 mg.L-1menunjukkan hasil terbaik dengan parameter kedinian munculnya tunas tercepat 45 hst, tunas terpanjang 7.6 cm, jumlah tunas terbanyak 7,33 tunas, dan persentase regenerasi 93,33%. Berdasarkan pengamatan histologi, kalus embriogenik berkembang menghasilkan mature embrio yang lebih banyak sehingga dapat menginduksi tunas yang banyak.
Empowering the Putri Keong Community Community through hydroponics of pakchoy mustard greens (Brassica rapa L.) and processed products Hardjo, Popy Hartatie
UN PENMAS (Jurnal Pengabdian Masyarakat untuk Negeri) Vol 4 No 2 (2024): UN PENMAS Vol 4 No 2
Publisher : LPPM Universitas Narotama

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Abstract

ABSTRACT Community service activities for Pokmas Putri Keong partners aim to increase the knowledge and skills of partners in developing business results in the field of hydroponic cultivation of pakcoy mustard greens and post-harvest handling of pakcoy mustard greens in both fresh and processed products. Based on the survey and situation analysis, the problem was identified as limited understanding and knowledge of hydroponic vegetable cultivation so that harvested products are less productive and only sold fresh directly to consumers and there is no proper post-harvest handling so they cannot be stored, therefore they will be developed into processed products. Community service programs include training in hydroponic and post-harvest cultivation and product processing, mentoring and provision of post-harvest equipment which can increase partner empowerment so that business results can be increased which can then grow even bigger by selling various processed products from harvests cultivated by the partners themselves. Key words: post-harvest, vegetable hydroponics
Effect of Light Color and Auxin on Callus Induction and Development in Amorphophallus muelleri Blume. Hardjo, Popy Hartatie; Thongiratama, Michael Anthony; Irawati, Fenny; Restanto, Didik Pudji
Vegetalika Vol 14, No 3 (2025): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106227

Abstract

Porang (Amorphophallus muelleri Blume) is a significant player in the international market, but its slow seedling growth rate is a major concern. This study, which holds significant implications for porang cultivation, aimed to accelerate the seedling growth phase through the induction of adventitious shoots from callus proliferation. We explored the use of plant growth regulators (PGRs), specifically auxins (NAA and 2,4-D) and cytokinins (BAP), in combination with variations in light color (white and white-blue). The observed parameters included the time of first callus and adventitious shoot emergence, callus growth, number of adventitious shoots, and callus coloration. Data were subjected to two-way analysis of variance (ANOVA) to evaluate the effects of plant growth regulator (PGR) type and light quality as independent variables. Statistical significance was assessed at a 95% confidence level using SPSS software. The combination of 1.0 ppm NAA and 0.5 ppm BAP in MS medium under white-blue light effectively accelerated callus induction from bulbil explants, with visible callus formation by day 8 and the fastest proliferation over 7 weeks. Under white light, the same PGR combination also promoted the earliest adventitious shoot formation by day 16 and yielded the highest number of shoots, totaling seven by the end of the culture period.
The effectiveness of growth regulators and light color spectrum on callus growth of Amorphophallus muelleri Blume. var. Madiun1: Efektivitas zat pengatur tumbuh dan spektrum warna cahaya terhadap pertumbuhan kalus Amorphophallus muelleri Blume. var. Madiun1 Wijaya, Agnes Natalia; Poernomo, Carlois; Savitri, Wina Dian; Irawati, Fenny; Hardjo, Popy Hartatie
AGROMIX Vol 14 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v14i2.3309

Abstract

Introduction: Plant Growth Regulators (PGRs) play a role in regulating organogenesis and morphogenesis in shoots, roots, and callus formation. Color spectrum of light is one of the quality light factors that affects plant physiological processes. This study aimed to determine the effect of cytokinin and auxin on Murashige-Skoog (MS) medium and light on callus induction and proliferation of porang (Amorphophallus muelleri Blume.) var. Madiun1. Methods: This study used completely randomized design, with the first factor was PGRs (combination auxin and cytokinin) and the second was color spectrum of light (white light, blue light, and its combination) during incubation. Variables observed were emergence time, color, texture, structure and calli growth, also shoots emerging from calli. Results: The results showed an interaction between PGRs with a combination of light color spectrum on callus growth. The fastest callus growth occurred in combination 5.0 mg.l-1 6-benzyl amino purine (BAP) with 0.2 mg.l-1 naphthalene acetic acid (NAA) which was incubated in a combination of white and blue light for 16 hour irradiation. The combination 5.0 mg.l-1 BAP with 0.2 mg.l-1 NAA was able to induce callus emergence time, and the shoots appearing were faster, whereas combination of white and blue light was able to accelerate callus emergence from bulbil and adventitious shoots emergence. Conclusion: The combination of white and blue light color spectrum for 16 hours irradiation can accelerate callus emergence from bulbil and adventitious shoots emerging from calli, and interaction with combination of 5.0 mg.l-1 BAP and 0.2 mg.l-1 NAA can accelerate porang’s callus growth.
Utilization of Trichoderma viride to Increase Patchouli Alcohol from Crude Extract of Acehnese Patchouli Leaves Hardjo, Popy Hartatie; Askitosari, Theresia Desy; Wijaya, Agnes Natalia; Edbert, Joshua; Artadana, Ida Bagus Made; Wittayathanarattana, Takon
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12057

Abstract

Acehnese Patchouli var. Sidikalang can produce patchouli oil, one of the country's sources of foreign exchange, and it is advantageous in many industries. However, patchouli alcohol (PA) content in patchouli oil is still low. The higher PA content determines the better quality of patchouli oil. So, delignification with Trichoderma viride was carried out to increase PA and yield of patchouli oil. The duration of delignification patchouli leaves is one factor affecting the increase in PA and yield. This study aimed to determine the effect of delignification duration on PA content and yield. The research method was completely randomized with 8 treatments (a combination of 2 treatments: with and without delignification, and 4 delignification durations: 0, 3, 6, and 9 days). The inoculum used for delignification was an inoculum ball, and then patchouli leaf samples were extracted using microwave-assisted extraction (MAE). Gas chromatography was carried out for PA analysis. Delignification of patchouli leaves, with a delignification time of 9 days, showed high PA and yield, which were (0.3129 ± 0.1557)% and (1.4543 ± 0.7717)%, respectively. The success of delignification at  9 days was also shown by increased phenolic content. Trichoderma viride is often utilized in delignification process, but the optimal duration of its delignification of Aceh patchouli leaves that can help improve patchouli oil quality is not yet known. This research is hoped to help related industries improve the secondary metabolites of plants, which has many benefits for the community. Implication/benefit for science development/society.
Direct Organogenesis of Different Explants of Aceh Patchouli (Pogostemon cablin Benth.) with Several BAP Concentrations Prayoga, Mohammad Candra; Soeparjono, Sigit; Dewanti, Parawita; Handoyo, Tri; Hardjo, Popy Hartatie; Restanto, Didik Pudji
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12839

Abstract

The patchouli plant (Pogostemon cablin Benth.) is a tropical herbaceous plant that produces essential oil. One of the problems is that the production is not yet optimal. Fulfillment of superior seedlings can help increase patchouli productivity. Conventional patchouli propagation through stem cuttings is ineffective and takes longer. Patchouli propagation can be done using a tissue culture approach via direct organogenesis to produce seedlings quickly and efficiently. Effective patchouli propagation methods and successful acclimatization are very important to research to support the propagation and breeding of patchouli plants. The aim of this research was to determine the best of BAP concentration in direct organogenesis of leaf and stem explants. The research design used a completely randomized series of hormone BAP, it has 5 levels, namely 0 mg/L (as control), 0.25 mg/L, 0.50 mg/L, 0.75 mg/L, and 1.0 mg/L. The explants used were the leaves and stems of Aceh patchouli. Plantlets are acclimatized in compost media and covering treatment. Based on the results of observations, the best BAP concentration is 0.25 mg/L with the initial observation parameters of the early emergence of shoots, number of shoots, and length of shoots on leaf explants were 10 daps, 35.33 shoots, and 2.83 cm respectively. The use of leaf explants showed a better response compared to stem explants. Patchouli plantlets were successfully acclimatized and can adapt to the ex vitro environment using the covering method. Successful patchouli propagation and high acclimatization can help produce effective patchouli seeds.
Co-Authors Arsyika, Izna Artadana, Ida Bagus Made Askitosari, Theresia Desy Edbert, Joshua Farlisa, Veronenci Yuliarbi Fenny Irawati Parawita Dewanti Poernomo, Carlois Prayoga, Mohammad Candra Restanto, Didik Pudji Sigit Soeparjono Thongiratama, Michael Anthony TRI HANDOYO Wijaya, Agnes Natalia Wina Dian Savitri, Wina Dian Wittayathanarattana, Takon