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All Journal Vegetalika Jurnal Pengabdian Masyarakat untuk Negeri (UN-PENMAS)
Popy Hartatie Hardjo
Lab. Bioteknologi Fak. Farmasi Univ. Surabaya
Agriculture, Biological Sciences & Forestry Humanities Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Social Sciences Other

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Optimasi Regenerasi Kalus Embriogenik Porang (Amorphophallus muelleri Blume) Melalui Kultur Suspensi Sel Menggunakan BAP (Benzyl Amino Purin) Restanto, Didik Pudji; Prayoga, Mohammad Candra; Arsyika, Izna; Farlisa, Veronenci Yuliarbi; Hardjo, Popy Hartatie
Vegetalika Vol 13, No 4 (2024): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.100708

Abstract

Porang Amorphophallus muelleri Blume merupakan tanaman umbi-umbian yang banyak dimanfaatkan sebagai bahan kosmetik dan diekspor untuk bahan baku industri. Perbanyakan porang secara konvensional mengalami masa dormansi. Perbanyakan porang melalui kultur jaringan dapat menghasilkan bibit yang cepat dan dalam jumlah yang banyak. Kultur jaringan porang dapat melalui somatik embriogenesis melalui pembentukan kalus dan poliferasi kalus dengan kultur suspensi sel. Optimasi regenerasi kalus hasil poliferasi kultur suspensi sel sangat penting untuk menunjang keberhasilan perbanyakan porang secara masal. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi BAP terhadap regenerasi kalus embriogenik porang Amorphophallus muelleri B. hasil kultur suspensi. Penelitian menggunakan rancangan acak lengkap dengan faktor konsentrasi BAP yang terdiri dari 4 taraf perlakuan antara lain 1 mg.L-1, 2 mg.L-1, 3 mg.L-1, dan 4 mg.L-1. Setiap perlakuan diulang sebanyak 5 ulangan. Analisis data penelitian menggunakan ANOVA dan uji lanjut dengan DMRT pada taraf kesalahan 5%. Analisis data menggunakan aplikasi SPSS statistics versi 26. Berdasarkan hasil penelitian, perlakuan BAP 1 mg.L-1menunjukkan hasil terbaik dengan parameter kedinian munculnya tunas tercepat 45 hst, tunas terpanjang 7.6 cm, jumlah tunas terbanyak 7,33 tunas, dan persentase regenerasi 93,33%. Berdasarkan pengamatan histologi, kalus embriogenik berkembang menghasilkan mature embrio yang lebih banyak sehingga dapat menginduksi tunas yang banyak.
Empowering the Putri Keong Community Community through hydroponics of pakchoy mustard greens (Brassica rapa L.) and processed products Hardjo, Popy Hartatie
UN PENMAS (Jurnal Pengabdian Masyarakat untuk Negeri) Vol 4 No 2 (2024): UN PENMAS Vol 4 No 2
Publisher : LPPM Universitas Narotama

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Abstract

ABSTRACT Community service activities for Pokmas Putri Keong partners aim to increase the knowledge and skills of partners in developing business results in the field of hydroponic cultivation of pakcoy mustard greens and post-harvest handling of pakcoy mustard greens in both fresh and processed products. Based on the survey and situation analysis, the problem was identified as limited understanding and knowledge of hydroponic vegetable cultivation so that harvested products are less productive and only sold fresh directly to consumers and there is no proper post-harvest handling so they cannot be stored, therefore they will be developed into processed products. Community service programs include training in hydroponic and post-harvest cultivation and product processing, mentoring and provision of post-harvest equipment which can increase partner empowerment so that business results can be increased which can then grow even bigger by selling various processed products from harvests cultivated by the partners themselves. Key words: post-harvest, vegetable hydroponics
Effect of Light Color and Auxin on Callus Induction and Development in Amorphophallus muelleri Blume. Hardjo, Popy Hartatie; Thongiratama, Michael Anthony; Irawati, Fenny; Restanto, Didik Pudji
Vegetalika Vol 14, No 3 (2025): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.106227

Abstract

Porang (Amorphophallus muelleri Blume) is a significant player in the international market, but its slow seedling growth rate is a major concern. This study, which holds significant implications for porang cultivation, aimed to accelerate the seedling growth phase through the induction of adventitious shoots from callus proliferation. We explored the use of plant growth regulators (PGRs), specifically auxins (NAA and 2,4-D) and cytokinins (BAP), in combination with variations in light color (white and white-blue). The observed parameters included the time of first callus and adventitious shoot emergence, callus growth, number of adventitious shoots, and callus coloration. Data were subjected to two-way analysis of variance (ANOVA) to evaluate the effects of plant growth regulator (PGR) type and light quality as independent variables. Statistical significance was assessed at a 95% confidence level using SPSS software. The combination of 1.0 ppm NAA and 0.5 ppm BAP in MS medium under white-blue light effectively accelerated callus induction from bulbil explants, with visible callus formation by day 8 and the fastest proliferation over 7 weeks. Under white light, the same PGR combination also promoted the earliest adventitious shoot formation by day 16 and yielded the highest number of shoots, totaling seven by the end of the culture period.
Co-Authors Arsyika, Izna Farlisa, Veronenci Yuliarbi Fenny Irawati Prayoga, Mohammad Candra Restanto, Didik Pudji Thongiratama, Michael Anthony