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All Journal Indonesian Journal of Community Dedication Universitas Muhammadiyah Yogyakarta Undergraduate Conference Proceeding
Suryani , Lilis
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Agriculture, Biological Sciences & Forestry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Nursing Public Health Social Sciences

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Optimization of pH to Bacteriocin Production by Lactic Acid Bacteria Growol Isolate Against Salmonella typhi Afaroh, Firda Rizka; Suryani , Lilis
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 3 No. 2 (2024): Crafting Innovation for Global Benefit
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v3i2.601

Abstract

Introduction – Growol is a traditional food from Yogyakarta which made from fermentation by Lactid Acid Bacteria (LAB) of cassava. LAB growol isolate as probiotics have ability to produce bacteriocins. Bacteriocins are useful for eradicating gram-positive and negative pathogenic bacteria. Bacteriocin activity is influenced by many factors, including pH, temperature, incubation time, and others, but the research about optimization of pH to produce bacteriocins that have maximum activity against S. typhi bacteria has been very limited. Purpose – This study aims to determine the ability of the bacteriocins produced by LAB growol isolate to inhibit the growth of S. typhi bacteria and to optimize the pH of the culture to increase its activity. Methodology/Approach – This research used a quasi-experimental method using post control group design. There were 5 samples of S. typhi bacteria which were divided into 5 groups. Group 1 was used as normal control without pH treatment. Groups 2, 3, 4, 5 were treatment with pH 2; 4; 6; 8. Each pH treatment was carried out with 4 repetitions. After the treatment, diameter of the inhibition zone of the disc was measured. The data obtained processed were using anova as a statistical analysis to see the difference in the sample mean. Findings – This study showed the results that optimizing of pH bacteriocin was optimum at inhibiting the growth of S. typhi bacteria is 2 with average diameter of inhibition zone is 11.10 mm. Originality/ Value/ Implication – The results of this study prove that pH has ability to affects bacteriocin activity produced by LAB growol isolate against S. typhi. LAB growol isolate has potential to produce bacteriocins to kill S. typhi.
Optimization of pH to Bacteriocin Production by Lactobacillus plantarum Against Methicillin-Resistant Staphylococcus aureus Khumaira, Naila El; Suryani , Lilis
Proceedings of Universitas Muhammadiyah Yogyakarta Graduate Conference Vol. 4 No. 2 (2025): Renewable Energy, Environment, and Technology for Sustainable Transformation
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/umygrace.v4i2.669

Abstract

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium that is resistant to beta-lactam antibiotics. MRSA is transmitted through skin/object contact and is often found in health care and the community. MRSA infection has a high mortality and morbidity rate because it causes sepsis, septic shock, respiratory failure, and amputation. Lactobacillus plantarum produces bacteriocin. The growth of both gram-positive and gram-negative bacteria can be inhibited by this bacteriocin. Purpose: To determine the effect of pH on the activity of bacteriocins produced by Lactobacillus plantarum against MRSA. Method: This research used quasi-experimental methods. L.plantarum was grown in liquid MRS media for 48 hours. Bacteriocin was obtained by centrifuging an MRS broth containing bacteria at a speed of 6000 rpm for 40 minutes. Using the Kirby Bauer method, the liquid formed was tested for its antibacterial effect against MRSA bacteria. Bacteriocins were treated with different pH, namely, 2, 4, 6, 8, and 10. The diameter of the inhibition zone formed during the testing process is measured in millimeters. Data were analyzed using the ANOVA method. Finding: The bacteriocin activity produced by Lactobacillus plantarum against MRSA shows an average diameter of the inhibition zone of 3 mm. The optimum broadest spectrum of antibacterial activity was shown in bacteriocins treated with pH 2. Conclusion: This study shows the results that pH does not affect the antibacterial activity of bacteriocins produced by L.plantarum against MRSA. Originality/Implication: The results of this study prove that various pH treatments do not affect the ability of the bacteriocin produced by L.plantarum to inhibit/kill MRSA.
Edukasi Dan Intervensi Gizi Dalam Pencegahan Stunting Pada Anak Di Kecamatan Ujung Bulu Kabupaten Bulukumba Adriani, Fitri; Erniawati, Erniawati; Sumarni, Sumarni; Suryani , Lilis; Angraeni, Ningsi
Indonesian Journal of Community Dedication Vol. 7 No. 2 (2025): Indonesian Journal of Community Dedication (IJCD)
Publisher : STIKES Nani Hasanuddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35892/community.v7i2.2721

Abstract

Stunting merupakan masalah kesehatan yang berdampak pada pertumbuhan dan perkembangan anak, terutama di daerah dengan tingkat ekonomi rendah. Penyebab utama stunting adalah kekurangan gizi kronis yang terjadi sejak masa kehamilan hingga usia dua tahun. Stunting dapat menghambat perkembangan kognitif dan fisik anak, yang berakibat pada rendahnya produktivitas di masa depan. Pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pemahaman ibu tentang stunting dan memberikan intervensi gizi yang sesuai guna mencegah stunting pada anak. Kegiatan ini dilaksanakan di Kecamatan Ujung Bulu, Kabupaten Bulukumba, dengan dihadiri oleh 20 ibu yang memiliki anak stunting. Metode yang digunakan meliputi penyuluhan, diskusi interaktif, serta pemberian makanan tambahan bergizi. Hasil kegiatan menunjukkan adanya peningkatan pengetahuan peserta sebesar 70% serta peningkatan kesadaran dalam menyediakan asupan gizi yang lebih baik bagi anak-anak mereka. Selain itu, intervensi gizi yang dilakukan dalam program ini menunjukkan peningkatan berat dan tinggi badan anak dalam satu bulan setelah pelaksanaan kegiatan. Kesimpulan dari kegiatan ini adalah bahwa edukasi dan intervensi gizi memberikan dampak positif terhadap pemahaman dan praktik gizi ibu serta pertumbuhan anak. Oleh karena itu, program serupa perlu dilanjutkan dan dikembangkan dengan kolaborasi berbagai pihak.
Co-Authors Adriani, Fitri Afaroh, Firda Rizka Angraeni, Ningsi Erniawati, Erniawati Khumaira, Naila El Sumarni Sumarni