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All Journal Indonesian Journal of Agricultural Research JURNAL PENGABDIAN KEPADA MASYARAKAT
Irda Safni
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Environmental Science Law, Crime, Criminology & Criminal Justice Library & Information Science Social Sciences Veterinary Other

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In Vitro Assay of Lytic Bacteriophage to Suppress the Growth of Ralstonia syzygii subsp. indonesiensis, the Causal Pathogen of Potato Wilt Disease Azizah, Nur; Irda Safni
Indonesian Journal of Agricultural Research Vol. 7 No. 2 (2024): InJAR, Vol. 7, No. 2, July 2024
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/injar.v7i2.14192

Abstract

Bacterial wilt disease, the one of the major diseases of potatoes, caused by Ralstonia syzygii subsp. indonesiensis (Rsi). Many efforts have been made to control bacterial wilt disease, including physical control, chemicals, and the use of bacteriophages. Previous studies have shown that bacteriophage application in controlling plant diseases is a fast-expanding area and has great potential to replace chemical methods. This study aims to determine the potential of lytic bacteriophage in suppressing the growth of Rsi in vitro. This study used a Non-Factorial, Completely Randomized Design with 3 replications and 6 treatment levels: R1 (Rsi isolate 1 without bacteriophage), R2 (Rsi isolate 1 with bacteriophage), R3 (Rsi isolate 2 without bacteriophage), R4 (Rsi isolate 2 with bacteriophage), R5 (Rsi isolate 3 without bacteriophage), and R6 (Rsi isolate 3 with bacteriophage). The results showed that bacteriophage could reduce the Rsi population at 24 hours by looking at the optical density (OD) value of 600 nm wavelength and growing on NA medium using the spread-plate method. The best treatment was at R6 with a population of 1 x 108cfu/ml Rsi. This research suggests that bacteriophage has the potential to suppress the growth of Rsi, which causes potato bacterial wilt disease, in vitro.
MENGENAL LEBIH DEKAT DENGAN PENYAKIT BUSUK PANGKAL DAN ATAS KELAPA SAWIT (BASAL DAN UPPER STEM ROT) Lisnawati; Wismaroh Sanniwati Saragih; Ahmad Rafiqi Tantawi; Hamidah Hanum; Suzanna Fitriani Sitepu; Irda Safni; Khairunnisa Lubis
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 14 No 2 (2024): Desember 2024
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30999/jpkm.v14i2.2949

Abstract

Basal stem rot and upper stem rot of oil palms caused by Ganoderma boninense are important oil palm diseases faced by oil palm plantations in Indonesia. For many years, basal stem rot was found to infect older oil palms that were more than 25 to 30 years old, but the disease began to appear in oil palms that were 10 to 15 years old and was even found infecting oil palm nurseries. Community service will be carried out in October 2023 in Gunung Ambat village, Sei Bingai District, Langkat Regency. The aim of this service is for the public to receive information about prevention and actions that will be taken by smallholder oil palm plantation farmers in the Sei Bingai sub-district, Langkat Regency. The expected target is for the community to distinguish attacks caused by G. boninense so that prevention can be carried out early and there is no decline in people's palm oil production. The community service method is carried out through lectures, discussions, and direct visits to people's oil palm plantations. The implementation of service activities was carried out in preparation by coordinating with community leaders and extension workers, as well as the implementation unit of the Sei Bingai sub-district agricultural service, Langkat Regency. Carrying out lectures and discussions with a team of lecturers and students targeting smallholder oil palm plantation farmers.
MENGENAL LEBIH DEKAT DENGAN PENYAKIT BUSUK PANGKAL DAN ATAS KELAPA SAWIT (BASAL DAN UPPER STEM ROT) Lisnawati; Wismaroh Sanniwati Saragih; Ahmad Rafiqi Tantawi; Hamidah Hanum; Suzanna Fitriani Sitepu; Irda Safni; Khairunnisa Lubis
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 14 No 2 (2024): Desember 2024
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30999/jpkm.v14i2.2949

Abstract

Basal stem rot and upper stem rot of oil palms caused by Ganoderma boninense are important oil palm diseases faced by oil palm plantations in Indonesia. For many years, basal stem rot was found to infect older oil palms that were more than 25 to 30 years old, but the disease began to appear in oil palms that were 10 to 15 years old and was even found infecting oil palm nurseries. Community service will be carried out in October 2023 in Gunung Ambat village, Sei Bingai District, Langkat Regency. The aim of this service is for the public to receive information about prevention and actions that will be taken by smallholder oil palm plantation farmers in the Sei Bingai sub-district, Langkat Regency. The expected target is for the community to distinguish attacks caused by G. boninense so that prevention can be carried out early and there is no decline in people's palm oil production. The community service method is carried out through lectures, discussions, and direct visits to people's oil palm plantations. The implementation of service activities was carried out in preparation by coordinating with community leaders and extension workers, as well as the implementation unit of the Sei Bingai sub-district agricultural service, Langkat Regency. Carrying out lectures and discussions with a team of lecturers and students targeting smallholder oil palm plantation farmers.
Co-Authors AHMAD RAFIQI TANTAWI Hamidah Hanum Khairunnisa Lubis Lisnawati Nur Azizah Saragih, Wismaroh Sanniwati Suzanna Fitriani Sitepu