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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Biologi UMA (JIBIOMA)
Marcellie, Jessica
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Analisis Koloni Bakteri pada Berbagai Sumber Air Minum Irawati, Wahyu; Marcellie, Jessica; Suhartono, Andreas Valiant; Marvella, Eunike Bunga
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 5, No 2 (2023): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v5i2.2322

Abstract

Drinking water is a fundamental necessity for human survival. In an era of advancing technology and busy lifestyles, bottled water, refillable water, and the use of water dispensers have become convenient choices. However, safe drinking water must be free from bacterial contaminants and chemicals. This research aims to analyze bacterial colonies from various sources of drinking water, such as bottled gallon water, hot water dispensers, and coldwater dispensers. The research results reveal that cold water dispensers have the highest number of bacterial colonies, totaling 1702, while hot water dispensers and gallon water contain only 2 and 13 bacterial colonies, respectively. Characteristics of the bacterial colonies in all samples include a yellow color, irregular margins, round shape, flat surface, and a hazy optical appearance. Additionally, Gram staining results indicate that the majority of bacterial isolates are Gram-negative, accounting for 76.92%. Factors influencing bacterial growth in drinking water from dispensers include insufficient cleanliness and inadequate sterilization during the installation of gallon water on the dispenser.
Protein Profile and Decolorization Potential of Copper-Resistant Klebsiella pneumonia CKJ 500 2.1.2 in Response to Textile Dyes and Copper Irawati, Wahyu; Pinontoan, Reinhard; Marcellie, Jessica; Valiant, Andreas; Sugata, Marcelia
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.584-595

Abstract

Heavy metals and synthetic dyes are major environmental pollutants, particularly in industrial effluents. The development of effective and safe bioremediation strategies to mitigate their ecological impact is therefore critical. In this study, the copper-resistant bacterium Klebsiella pneumoniae CKJ 500 2.1.2 was investigated for its capacity to decolorize textile dyes—specifically malachite green—and its associated enzymatic activities were characterized. Bacterial resistance was assessed using Luria–Bertani agar containing varying concentrations of copper and dyes. Decolorization efficiency was evaluated spectrophotometrically, protein expression was analyzed using SDS-PAGE, and dye degradation products were identified using gas chromatography–mass spectrometry (GC-MS). The strain exhibited high tolerance to both copper and dyes, achieving 99.4% decolorization of malachite green and 81% for Congo red. The presence of copper inhibited the decolorization of most dyes, except malachite green and methylene blue. SDS-PAGE analysis identified three key enzymes: laccase (~60 kDa), manganese peroxidase (~39 kDa), and azoreductase (~22 kDa). GC-MS revealed both toxic and non-toxic degradation intermediates, indicating partial detoxification. These findings highlight the potential of K. pneumoniae CKJ 500 2.1.2 for bioremediation of dye-contaminated effluents. However, further research is required to elucidate the complete enzymatic pathways involved and to ensure environmentally safe dye degradation.
Co-Authors Marvella, Eunike Bunga Reinhard Pinontoan Sugata, Marcelia Suhartono, Andreas Valiant Valiant, Andreas WAHYU IRAWATI