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All Journal EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi BioEksakta : Jurnal Ilmiah Biologi Unsoed
Nuraeni, Siti Panesya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Catatan Persebaran Russula cf. cyanoxantha di Hutan Kampus Institut Pertanian Bogor Putra, Ivan Permana; Haryadi, Silva Restiana; Nafsahan, Hury Khastina; Emillio, Guido Qais; Nuraeni, Siti Panesya; Anisyah, Fara Ayu; Khairani, Hafifatunil
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4016

Abstract

Russula cyanoxantha is one of a group of ectomycorrhizal-forming mushrooms. This species has a main distribution in the four seasons region. Until now, there is limited information on the distribution and taxonomic studies of this species of mushroom in Indonesia. Therefore, this study aimed to add information on the morphological characteristics, taxonomy, and distribution of R. cyanoxantha in Indonesia. This study was conducted in the campus forest of IPB University. Fresh fruiting bodies were observed in the field and in the laboratory. The parameters observed were macroscopic and microscopic characters of the collected samples. The mushroom found had cap colored in shades of white, gray, and purplish. The lamellae are white to cream colored and adnate to the cap, cylindrical stipe. Spores are round to oval with ornaments in the form of small spines on their cell walls. The results of this study are expected to contribute towards the addition of data on Russula mushrooms in Indonesia.
Isolasi, Karakterisasi dan Identifikasi Bakteri Penghasil Hemolisin pada Permukaan Daun Pohpohan (Pilea Melastomoides (Poir.) Wedd.) Nuraeni, Siti Panesya; Budiarti, Sri; Priyanto, Jepri Agung
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.13222

Abstract

Pohpohan (Pilea melastomoides (Poir.) Wedd.) umumnya dikonsumsi masyarakat sebagai lalapan. Sayuran segar yang dikonsumsi secara langsung dapat membawa bakteri patogen bawaan pangan. Penelitian ini bertujuan mengisolasi bakteri, mengarakterisasi, mengidentifikasi bakteri penghasil hemolisin pada permukaan daun pohpohan. Kuantifikasi bakteri pada media Blood Agar menunjukkan jumlah bakteri patogen sebanyak 6,2 x108 CFU/g. Koloni bakteri dengan morfologi yang berbeda dipilih dan dimurnikan untuk dikarakterisasi lebih lanjut meliputi karakter morfologi koloni dan selnya. Sebanyak delapan isolat bakteri terpilih memiliki warna koloni yang sama yaitu putih, namun memiliki bentuk, tepian, elevasi, karakteristik optik, dan permukaan koloni yang bervariasi. Semua isolat bakteri tersebut tergolong bakteri Gram positif berbentuk basil dengan penataan sel berantai atau tunggal. Kedelapan isolat terpilih mampu melisiskan sel darah merah (hemolisis positif), 6 isolat diantaranya tergolong ke dalam β-hemolisis, dan 2 isolat lainnya tergolong a-hemolisis. Identifikasi molekuler berdasarkan gen 16S rRNA menunjukkan empat isolat (PA7I1, PA8I5, PA8I6.2, PA9I3) memiliki kemiripan sekuens yang tinggi (similarity: lebih dari 99%) dengan bakteri Bacillus paramycoides, dua isolat (PA8I7, PA9I5) teridentifikasi sebagai Bacillus subtilis, dan dua isolat lainnya (PA8I6, PA914) teridentifikasi sebagai Bacillus cereus dan Bacillus tequilensis. Kata kunci: Bacillus, hemolisis, pohpohan, sayuran segar
Catatan Persebaran Russula cf. cyanoxantha di Hutan Kampus Institut Pertanian Bogor Putra, Ivan Permana; Haryadi, Silva Restiana; Nafsahan, Hury Khastina; Emillio, Guido Qais; Nuraeni, Siti Panesya; Anisyah, Fara Ayu; Khairani, Hafifatunil
EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi, dan Mikrobiologi Vol 8 No 1 (2023): Ekotonia: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi
Publisher : Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/ekotonia.v8i1.4016

Abstract

Russula cyanoxantha is one of a group of ectomycorrhizal-forming mushrooms. This species has a main distribution in the four seasons region. Until now, there is limited information on the distribution and taxonomic studies of this species of mushroom in Indonesia. Therefore, this study aimed to add information on the morphological characteristics, taxonomy, and distribution of R. cyanoxantha in Indonesia. This study was conducted in the campus forest of IPB University. Fresh fruiting bodies were observed in the field and in the laboratory. The parameters observed were macroscopic and microscopic characters of the collected samples. The mushroom found had cap colored in shades of white, gray, and purplish. The lamellae are white to cream colored and adnate to the cap, cylindrical stipe. Spores are round to oval with ornaments in the form of small spines on their cell walls. The results of this study are expected to contribute towards the addition of data on Russula mushrooms in Indonesia.
Co-Authors Anisyah, Fara Ayu Emillio, Guido Qais Haryadi, Silva Restiana Ivan Permana Putra Jepri Agung Priyanto, Jepri Agung Khairani, Hafifatunil Nafsahan, Hury Khastina Sri Budiarti Poerwanto