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All Journal HAYATI Journal of Biosciences SAINS DAN MATEMATIKA Jurnal Ilmu Dasar Jurnal Sumberdaya Hayati BioEksakta : Jurnal Ilmiah Biologi Unsoed Makara Journal of Science
Jepri Agung Priyanto, Jepri Agung
Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Immunology & microbiology Mathematics Veterinary

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Flavonoids Production Capability Test of Tea Mistletoe (Scurrula atropurpurea BL . Dans) Endophytic Bacteria Isolates Priyanto, Jepri Agung; Pujiyanto, Sri; Rukmi, Isworo
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 4 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.599 KB)

Abstract

Tea Mistletoe (S. Atropurpurea BL. Dans) is a medicinal plant species used as anticancer because it contains some flavonoids compounds are chalcones, c-glycoflavonols, chatechin and quercetin. The ability of endophytic bacteria to produce similar bioactive compounds with its host plant is potential source to get flavonoids compounds. This research aims to know ability of endophytic bacteria in produce flavonoids compounds in vitro. Each isolate was fermented in submerged culture with 0.1% soluble starch, 0.5% pepton, and 0.15% yeast extract medium for 5 days, then extracted with ethyl acetate. Flavonoid content of the extract then was tested qualitatively and confirmation test used thin layer chromatography. Qualitative test results showed that crude extract from isolates B4, B5, B10, B17, and B19 positive containing flavonoids. The most potent extract were B10 and B19 tested by thin layer chromatography. Two of these extracts had the same Rf value with quercetin, thus endophytic bacteria from tea mistletoe can produce flavonoids in vitro.
Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi Priyanto, Jepri Agung; Hening, Egiyanti Nur Widhia; Permatasari, Vera; Prastya, Muhammad Eka; Hasidu, La Ode Abdul Fajar; Primahana, Gian
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.39244

Abstract

Soil bacteria could be the promising source of secondary metabolites with diverse pharmacological activities. Bacteria coded as P1 and M7 were isolated from soil obtained from Muna Island, Southeast Sulawesi. This research aimed to evaluate the antioxidant activity, cytotoxic property, and identify the chemical profile of the extract derived from soil bacteria P1 and M7. Based on 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, extract derived from P1 isolate was moderately active against ABTS free radical with IC50 of 126.22±10.82 µg/mL, and less active on DPPH (IC50: 721.95±16.59 µg/mL), while M7 extract was less active towards DPPH and ABTS. Interestingly, these extracts in the concentration of 100 µg/mL were cytotoxic to breast cancer cell (MCF-7), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MCF-7 viability was decreased up to 69.83±1.46 % and 61.49±0.76 % after treated with P1 and M7 extract, respectively. The extracts were also induced membrane disruption and apoptosis of MCF-7 cells. The extracts contained phenol (9.52±0.22 and 10.41±0.09 mg EAG/g extract, for P1 and M7, respectively) and flavonoids (9.16±0.16 and 9.29±0.24 mg EQ/g extract, for P1 and M7, respectively). According to Gas Chromatography Mass Spectrometry (GC-MS) analysis, Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) that previously well known as antioxidant and cytotoxic compounds, were found in the M7 and P1 extract. This compound may play a vital role in the radical scavenging and cytotoxic properties of P1 and M7 extracts. The results of this study indicate that P1 and M7 extracts could be the potential source of antioxidant and cytotoxic compounds and need to be further developed for pharmaceutical use.
Virulence Evaluation of Aeromonas spp. KS-1 Isolated from Kitchen Sponge using Omphisa fuscidentalis Larvae Ilsan, Noor Andryan; Inggrain, Maulin; Nurfajriah, Siti; Yunita, Melda; Priyanto, Jepri Agung; Ramanda, Viqih
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.613-620

Abstract

Aeromonas spp. causes the human diseases including diarrhea, gastroenteritis, and bacteremia. Aeromonas spp. can be found in kitchen sponge, one of the reservoirs for food-borne bacterial pathogens. Virulence study of Aeromonas spp. in vivo in animal model is important since the animal model can mimic manifestasions in human infections. Omphisa fuscidentalis was chosen for alternative virulence model, since they are in the same taxonomical order with the well-known infections model, Galleria mellonella. Bacterial isolation and selection of kitchen sponge used Brain Heart Infusion agar and Endo Agar, respectively. Bacterial virulence of KS-1 was injected into Omphisa fuscidentalis larvae. Survival percentage and melanization score of infected larvae were evaluated. Hemolymph of larvae with melanization score of 1 and 4 were stained with Giemsa method to observe the hemocyte changes. Bacterial identification of isolate KS-1 based on 16S rRNA sequence resulted in 96.9% identity to Aeromonas spp. strain VS7. Isolate KS-1 injection to O. fuscidentalis revealed higher bacterial dosage resulting more severe symptoms to the larvae according to survival percentage and melanization score. However, statistical analysis showed evaluation of melanization score could distinguish larvae with 106 and 107 CFU/larva dosage injection, while evaluation of survival percentage could not. Hemocyte of larvae with melanization score 1 had larger and more cytoplasmic vacuolization than the score 4 (healthy larvae). Omphisa fuscidentalis is an alternative of insect model for bacterial infections with survival percentage and melanization score as the evaluation. Cytoplasmic vacuolization of hemocyte can be used as larvae’s health indicator in a cellular level.
Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp. Maherani, Vincentia Fenice Angger; Mubarik, Nisa Rachmania; Priyanto, Jepri Agung; Putra, Ivan Permana
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.725-732

Abstract

Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.
Karakterisasi Bakteri Tanah Penghasil Enzim Hidrolitik yang Diisolasi dari Pulau Muna, Sulawesi Tenggara, Indonesia: Characterization of Hydrolytic Enzyme Producing-Bacteria Isolated from Soil of Muna Island, Southeast Sulawesi, Indonesia Wibowo, Muhammad Arya; Priyanto, Jepri Agung; Budiarti, Sri
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.55-60

Abstract

Soil bacteria are considered a promising source of extracellular enzymes, such as amylase, cellulase, and protease. In this study, 15 bacterial strains isolated from the soil of Muna Island, Southeast Sulawesi, Indonesia, were preliminary screened for amylolytic, cellulolytic, and proteolytic activities. The present study aimed to characterize cellular morphology, determine hemolytic characteristics, and to screen amylolytic, proteolytic, and cellulolytic activities of bacterial strains isolated from the soil of Muna Island. Based on the Gram staining procedure, 15 bacterial strains exhibited diverse cellular morphologies. Ten out of 15 strains belonged to Gram-positive bacilli, while the others belonged to Gram-negative bacteria (bacilli or cocci). Four strains, encoded as M7, P4, P5, and P7, were hemolytic negative. These potential strains exhibited different capabilities in producing extracellular enzymes. The four strains were capable of producing protease, while amylase and cellulase were only produced by two strains (M7 and P7). These strains have good prospects for further study and development for industrial and biotechnological applications.
Aktivitas Anti-Mycobacterium Ekstrak Lada Hitam (Piper nigrum) dan Bawang Bombai (Allium cepa L.) serta Kombinasinya Ulfa, Aulia; Nabilla, Yasmin Nuha; Riswanti, Mellyana; Septianingrum, Danik; Alzahra, Ziqri; Priyanto, Jepri Agung
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 4 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.4.13221

Abstract

Eksplorasi senyawa aktif dari tanaman terutama dari lada hitam (Piper nigrum) dan bawang bombai (Allium cepa L.) dibutuhkan untuk mengatasi infeksi tuberkulosis (TB) sebagai alternatif dari antibiotik yang tersedia saat ini. Penelitian ini bertujuan untuk mengevaluasi aktivitas anti-Mycobacterium dari ekstrak P. nigrum, A. cepa, dan kombinasinya serta mengidentifikasi profil metabolitnya. Metabolit yang berasal dari tanaman ini diekstraksi menggunakan teknik maserasi dengan etanol 70%. Ekstrak A. cepa menunjukkan hasil ekstraksi yang lebih tinggi yaitu 19,18% daripada P. nigrum (8,15%). Di antara lima formula yang berbeda (F1-F5), F5 (hanya mengandung ekstrak P. nigrum) menunjukkan aktivitas anti-Mycobacterium terkuat dengan zona penghambatan 12±0 mm dengan metode difusi cakram. Ekstrak tersebut juga menunjukkan minimum inhibitory concentration (MIC) terkuat dibandingkan dengan formula lain, dengan nilai MIC sebesar 0,312 mg/mL. Selain itu, semua formula menunjukkan nilai minimum bactericidal concentration (MBC) lebih besar dari 10 mg/mL. Analisis LC-MS/MS mengidentifikasi bahwa kedua ekstrak tanaman ini memiliki profil metabolit yang berbeda, beberapa di antaranya telah dilaporkan sebagai agen antimikroba. Hasil penelitian ini menunjukkan bahwa ekstrak yang berasal dari P. nigrum lebih aktif daripada ekstrak A. cepa dan kombinasinya. Ekstrak P. nigrum sangat menjanjikan untuk studi dan pengembangan lebih lanjut sebagai agen anti-Mycobacterium berbasis tanaman. Kata kunci: Antibakteri, bawang bombai, lada hitam, Mycobacterium smegmatis, tuberkulosis
Isolasi, Karakterisasi, dan Identifikasi Bakteri Penghasil Hemolisin dari Penderita Gingivitis: Isolation, Characterisation, and Identification of Hemolysin-Producing Bacteria from Gingivitis-Diagnosed Patient Sutandi, Michellia Salmaa Ashilla; Priyanto, Jepri Agung; Budiarti, Sri
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.162-169

Abstract

Gingivitis is a gum inflammation caused by pathogenic bacterial infection. One of the virulence factors of pathogenic bacteria is hemolysin which plays an important role in lysing the red blood cells. This study aimed to isolate, characterise, and identify hemolysin-producing bacteria from a gingivitis-diagnosed patient. Quantification of the bacterial growth in blood agar base media showed that the number of bacteria on the gums of gingivitis patient was 1.63 × 107 CFU/ml. Seven bacterial isolates with different colony and cellular morphology were selected. Of 7 isolates, 3 isolates were β hemolytic, 3 isolates α hemolytic, and 1 isolate non-hemolytic. Six isolates with hemolytic activity were selected for colony and cellular morphology characterisation. Based on Gram-staining procedure, all six isolates belong to bacilli Gram-positive bacteria. Molecular identification with 16S rRNA gene revealed that these isolates were closely related to Bacillus spp., such as Bacillus cereus, Bacillus altitudinis, and Bacillus tequilensis.
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria Prastya, Muhammad Eka; Simbolon, Sumihartati; Priyanto, Jepri Agung; Hasidu, La Ode Abdul Fajar; Permatasari, Vera; Primahana, Gian; Dewi, Rizna Triana; Kristiana, Rhesi; Suryanti, Erma
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.12-26

Abstract

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.
Isolasi, Karakterisasi dan Identifikasi Bakteri Penghasil Hemolisin pada Permukaan Daun Pohpohan (Pilea Melastomoides (Poir.) Wedd.) Nuraeni, Siti Panesya; Budiarti, Sri; Priyanto, Jepri Agung
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.13222

Abstract

Pohpohan (Pilea melastomoides (Poir.) Wedd.) umumnya dikonsumsi masyarakat sebagai lalapan. Sayuran segar yang dikonsumsi secara langsung dapat membawa bakteri patogen bawaan pangan. Penelitian ini bertujuan mengisolasi bakteri, mengarakterisasi, mengidentifikasi bakteri penghasil hemolisin pada permukaan daun pohpohan. Kuantifikasi bakteri pada media Blood Agar menunjukkan jumlah bakteri patogen sebanyak 6,2 x108 CFU/g. Koloni bakteri dengan morfologi yang berbeda dipilih dan dimurnikan untuk dikarakterisasi lebih lanjut meliputi karakter morfologi koloni dan selnya. Sebanyak delapan isolat bakteri terpilih memiliki warna koloni yang sama yaitu putih, namun memiliki bentuk, tepian, elevasi, karakteristik optik, dan permukaan koloni yang bervariasi. Semua isolat bakteri tersebut tergolong bakteri Gram positif berbentuk basil dengan penataan sel berantai atau tunggal. Kedelapan isolat terpilih mampu melisiskan sel darah merah (hemolisis positif), 6 isolat diantaranya tergolong ke dalam β-hemolisis, dan 2 isolat lainnya tergolong a-hemolisis. Identifikasi molekuler berdasarkan gen 16S rRNA menunjukkan empat isolat (PA7I1, PA8I5, PA8I6.2, PA9I3) memiliki kemiripan sekuens yang tinggi (similarity: lebih dari 99%) dengan bakteri Bacillus paramycoides, dua isolat (PA8I7, PA9I5) teridentifikasi sebagai Bacillus subtilis, dan dua isolat lainnya (PA8I6, PA914) teridentifikasi sebagai Bacillus cereus dan Bacillus tequilensis. Kata kunci: Bacillus, hemolisis, pohpohan, sayuran segar
Antibacterial Activity of Endophytic Actinobacteria Isolated from Ziziphus mauritiana Against Multidrug-Resistant Strains Khairani, Hafifatunil; Priyanto, Jepri Agung; Prastya, Muhammad Eka; Retnowati, Dwi
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Increasing resistance to existing antibiotics has led to a need for new alternative sources. Among the possibilities that need to be explored is the endophytic actinobacteria associated with Ziziphus mauritiana. The present study aimed to evaluate the antibacterial activity of endophytic actinobacteria isolated from Z. mauritiana against five multidrug-resistant bacterial strains. Thirty actinobacteria isolates were obtained from the roots, stem, and leaves of the plant using the serial dilution technique. Using a double-layer agar test, we found that colonies from five of the 30 isolates (16%) exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and the M18 strain of Bacillus subtilis. Interestingly, the crude extracts derived from four selected isolates (BBK 1, DBK 2, DBK 3, and DBSB 1) showed inhibitory activity against MRSA, with inhibition zone diameters ranging from 8 (±0.8) to 11 (±2.9) mm. The highest inhibition zone was seen in DBK 2. This had a zone diameter of 11 (±2.9) mm, which is not signifi-cantly different from the zone diameter of 12 (±0) mm seen in tetracycline. The minimum inhibitory concentrations (MICs) were determined using the micro-broth dilution technique. This showed MICs ranging from 2,500–5,000 μg/mL in the four extracts. Based on 16S ribosomal ribonucleic acid (rRNA) sequences, the four selected isolates were found to belong to the Streptomyces spp., with similarity values exceeding 99%. These four isolates require further study as potential antibiotic sources, particularly against MRSA.
Co-Authors Alzahra, Ziqri Dwi Retnowati Getriani, Zerina Hasidu, La Ode Abdul Fajar Hening, Egiyanti Nur Widhia Ilsan, Noor Andryan Inggrain, Maulin Ivan Permana Putra Khairani, Hafifatunil Maherani, Vincentia Fenice Angger Melda Yunita MG Isworo Rukmi Nabilla, Yasmin Nuha NISA RACHMANIA MUBARIK Nuraeni, Siti Panesya Permatasari, Vera Prastya, Muhammad Eka Primahana, Gian Ramanda, Viqih Rhesi Kristiana Riswanti, Mellyana Rizna Triana Dewi Septianingrum, Danik Simbolon, Sumihartati Siti Nurfajriah Sri Budiarti Poerwanto Sri Pujiyanto Suryanti, Erma Sutandi, Michellia Salmaa Ashilla Ulfa, Aulia Wibowo, Muhammad Arya