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All Journal AGRIVITA, Journal of Agricultural Science Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Khofifa, Rendryana Aulia Nur
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions

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Respon Perlakuan Asam Jasmonat, Asam Salisilat dan Kitosan terhadap Produksi Metabolit Sekunder pada Kalus Padi Daun Berpigmen Khofifa, Rendryana Aulia Nur; Ubaidillah, Mohammad
Agriprima : Journal of Applied Agricultural Sciences Vol 8 No 1 (2024): MARCH
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/agriprima.v8i1.599

Abstract

Padi Black Madras (Oryza sativa L.) merupakan padi hias yang selain biasa digunakan sebagai ornamen, juga dapat digunakan sebagai sumber pharmaceutical yang bermanfaat bagi kesehatan manusia. Namun saat ini belum banyak diketahui potensialnya menjadi sumber pharmaceutical seperti metabolit sekunder. Metabolit sekunder yang dihasilkan oleh padi black madras ini dapat diambil melalui kultur jaringan dengan cara elisitasi. Penelitian ini dilakukan untuk mengetahui respon kalus padi dan produksi metabolit sekunder pada padi Black Madras terhadap pemberian asam jasmonat, asam salisilat, dan kitosan. Hasil penelitian ini menunjukkan pemberian elisitor memberikan respon pada kalus padi dan produksi metabolit sekunder padi black madras terhadap pemberian asam jasmonat, asam salisilat, dan kitosan. Varietas terbaik didapatkan pada varietas IR64 sebagai kontrol daripada Black madras dengan perlakuan dengan kandungan fenolik (0,1548 mg GAE/g) dan flavonoid (0,2830 mg QE/g) terbaik ada pada media dengan pemberian Asam Jasmonat 10ppm. Sedangkan pada antosianin dengan pemberian yang terbaik adalah  Kitosan 50ppm  (PC: 2,852 CV/g FW dan PP: 0,538 CV/ test tube). Varietas Black madras lebih cenderung dalam peningkatan biomassa dengan kandungan fenolik (0,1165 mg GAE/g) dan flavonoid (0,2107 mg QE/g) pada perlakuan kontrol meskipun dalam produksi antosianin cukup tinggi dengan Kitosan 50ppm  (PC: 2,832 CV/g FW) dan Asam Salisilat 10ppm (PP: 0,440 CV/ test tube) lebih daripada perlakuan kontrol.
EFFICIENT REGENERATION LOCAL BANANA TISSUE CULTURE USING FLORAL APICES BY CYTOKININ COMBINATION Makhsunah, Isnina Tasya; Habibi, Ali Mukit; Khofifa, Rendryana Aulia Nur; Ubaidillah, Mohammad
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.3123

Abstract

Banana (Musa sp.) is an important crop that is widely cultivated and consumed in Indonesia. Banana plants production can be increased through tissue culture with the use of explants and the right combination of hormones. This study was conducted to determine the use of flower apices explants on local banana varieties using a combination of cytokinin hormones containing BAP (Benzylaminopurine) and several levels of TDZ (Thidiazuron). The result of this research was analyzed using a one-way analysis of variance. The hormone combination in this study consists of MS + BAP 2 mg/L + three concentrations of TDZ (2 mg/L, 2.5 mg/L, and 3 mg/L) as a regeneration medium. Three local banana varieties (Kepok Putih, Kepok Kuning, and Pisang Susu) was cultured on the three combination medium. The results showed that the use of flower apices explants contained in banana flowers grown on MS media with a combination of cytokinin hormones could increase the efficiency of explant regeneration. Media MS + BAP 2 mg/L + TDZ 3 mg/L gave the best regeneration rate for Kepok Putih (62.50%), Kepok Kuning (62.50%) and Banana Susu (56.25%) compared to TDZ 2. mg/L and TDZ 3 mg/L. The novelty of this study can provide information in the propagation of local banana plants using flower apices explants.
Response of Rice Somatic Embryogenesis to Exogenous Melatonin About Its Role in Scavenging Reactive Oxygen Species Ubaidillah, Mohammad; Al Ayyubi, Nabila Nur Aisyah; Khofifa, Rendryana Aulia Nur; Dewanti, Parawita
AGRIVITA Journal of Agricultural Science Vol 46, No 1 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i1.4060

Abstract

The success rate of explant morphogenesis in plant breeding using tissue culture techniques is frequently plagued by browning due to the oxidation of phenolic compounds. The cumulated amount of reactive oxygen species (ROS) drives the oxidation of phenolic compounds. Melatonin is reported to take a part in modulating the regulation of antioxidant gene expression, reducing the accumulation of reactive oxygen species, and enhancing the efficacy of tissue culture. This study aims to determine the optimal melatonin concentration on the efficiency of plantlet regeneration and expression of the antioxidant resistance gene in rice callus. This study utilizes rice TN1, Gogo Niti II, Ketan Hitam, and Cigeulis cultivars. Melatonin at 0, 10, and 15 µM concentrations is supplemented in plantlet regeneration media. Rice antioxidant-related genes, Mn-SOD, Cu/ZnSOD, Cytosolic APX, CAT, GPOD, OsAPX, and OsCATA, expressed after melatonin supplementation. Melatonin concentration at 10 µM generates the highest expression of all tested genes in TN1 compared to other varieties. The cumulated amount of hydrogen peroxide (H2O2) shows that Melatonin has the potential to increase the proportion of plant regeneration in Cigeulis (90.48%) and Ketan Hitam (91.67%) varieties with a concentration of 10 µM and in TN1 (94.44%) and Gogo Niti II (80%) at a concentration of 15 µM.
Co-Authors Al Ayyubi, Nabila Nur Aisyah Habibi, Ali Mukit Makhsunah, Isnina Tasya Parawita Dewanti Ubaidillah, Mohammad