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All Journal Seminar Nasional Teknik Kimia Kejuangan
Tyas, Sunia Rahma Cahyaning
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Environmental Science

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Pengaruh Variasi Bahan Pengendap pada Proses Purifikasi Enzim Bromelin dari Bonggol Nanas (Ananas Comosus L.) Triastuti, Warlinda Eka; Tyas, Sunia Rahma Cahyaning; Zahrah, Hanifah Fauziyah; Bennani, Kabira; Cahyani, Erika Desi; Ramadita, Tatya Annur; Rahayu, Adel Frisca
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

Waste from processed pineapples (pineapple core) is currently underutilized, despite the high content of the bromelain enzyme found in the fruit. This research aims to determine the effect of precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the resulting enzyme yield and to analyze the effect of the precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the bromelain enzyme activity. The variables used in this study were a 0.1 M phosphate buffer, 55%; 60%; 65%; 70%; 75% aseton concentration, 5%; 10%; 15%; 20%; 25% NaCl concentration, and 60%; 65%; 70%; 75%; 80% ethanol concentration. This study will be conducted in three stages, namely Extraction, Purification, and Drying. The highest yield was obtained with 60% aseton concentration (1.44%), 65% ethanol concentration (1.42%), and 15% NaCl concentration (2.2%). The optimal enzyme activity was obtained with 60% aseton concentration (20225.616 U/gr), 65% ethanol concentration (6266.765 U/gr), and 15% NaCl concentration (2281.357 U/gr). In conclusion, the best result for producing bromelain enzyme powder from pineapple cores is by using a 60% aseton concentration solvent.
Penentuan Kadar Sulfat pada Air Sumur di Wilayah Surabaya Menggunakan Spektrofotometer Agustiani, Elly; Triastuti, Warlinda Eka; Sampurno, Ade Citra Oktaviana Elok; Zahrah, Hanifah Fauziyah; Tyas, Sunia Rahma Cahyaning; Santoso, Yustia Dwi Fitria; Rahmawati, Affy Milatur; Martasari, Amelia; Wintara, Ellen Septia; Amali, Hafid
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

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Abstract

The quality of drinking water can be assessed based on three aspects: physical, chemical, and microbiological aspects. One of the chemical aspects in determining water quality is the sulfate content. UV-Vis spectrophotometry method has been widely used to analyze sulfate content. UV-Vis spectrophotometry is a spectroscopic analysis technique that uses an electromagnetic wave source with ultraviolet (UV) for wavelength (190-380 nm) and visible light with wavelength (380-780 nm). The purpose of this study is to determine the absorbance and concentration of samples using spectrophotometric analysis, calculate the sulfate content in the samples, and compare it with the Indonesian Ministry of Health Regulation Permenkes RI No. 32//2017. The research stages include calibration, calibration curve creation, making standard H2SO4 solutions, measuring the absorbance of the standard solutions, and analyzing the sulfate content in water samples. The research results showed that the sulfate concentration in the Bangkalan, Nginden, Sidoarjo, Keputih, and Kapas Madya well water samples were 14.0817, 27.551, 29.1837, 49.0817, 54.6939 ppm, respectively. Based on the Indonesian Ministry of Health Regulation Permenkes RI No. 32//2017, the maximum sulfate content in water is 400 mg/L, thus it can be concluded that the water samples in those areas have met the clean water standards.
Pengaruh Variasi Bahan Pengendap pada Proses Purifikasi Enzim Bromelin dari Bonggol Nanas (Ananas Comosus L.) Triastuti, Warlinda Eka; Tyas, Sunia Rahma Cahyaning; Zahrah, Hanifah Fauziyah; Bennani, Kabira; Cahyani, Erika Desi; Ramadita, Tatya Annur; Rahayu, Adel Frisca
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Waste from processed pineapples (pineapple core) is currently underutilized, despite the high content of the bromelain enzyme found in the fruit. This research aims to determine the effect of precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the resulting enzyme yield and to analyze the effect of the precipitating agents on the purification process of bromelain enzyme production using a phosphate buffer on the bromelain enzyme activity. The variables used in this study were a 0.1 M phosphate buffer, 55%; 60%; 65%; 70%; 75% aseton concentration, 5%; 10%; 15%; 20%; 25% NaCl concentration, and 60%; 65%; 70%; 75%; 80% ethanol concentration. This study will be conducted in three stages, namely Extraction, Purification, and Drying. The highest yield was obtained with 60% aseton concentration (1.44%), 65% ethanol concentration (1.42%), and 15% NaCl concentration (2.2%). The optimal enzyme activity was obtained with 60% aseton concentration (20225.616 U/gr), 65% ethanol concentration (6266.765 U/gr), and 15% NaCl concentration (2281.357 U/gr). In conclusion, the best result for producing bromelain enzyme powder from pineapple cores is by using a 60% aseton concentration solvent.
Penentuan Kadar Sulfat pada Air Sumur di Wilayah Surabaya Menggunakan Spektrofotometer Agustiani, Elly; Triastuti, Warlinda Eka; Sampurno, Ade Citra Oktaviana Elok; Zahrah, Hanifah Fauziyah; Tyas, Sunia Rahma Cahyaning; Santoso, Yustia Dwi Fitria; Rahmawati, Affy Milatur; Martasari, Amelia; Wintara, Ellen Septia; Amali, Hafid
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2023: PROSIDING SNTKK 2023
Publisher : Seminar Nasional Teknik Kimia "Kejuangan"

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The quality of drinking water can be assessed based on three aspects: physical, chemical, and microbiological aspects. One of the chemical aspects in determining water quality is the sulfate content. UV-Vis spectrophotometry method has been widely used to analyze sulfate content. UV-Vis spectrophotometry is a spectroscopic analysis technique that uses an electromagnetic wave source with ultraviolet (UV) for wavelength (190-380 nm) and visible light with wavelength (380-780 nm). The purpose of this study is to determine the absorbance and concentration of samples using spectrophotometric analysis, calculate the sulfate content in the samples, and compare it with the Indonesian Ministry of Health Regulation Permenkes RI No. 32//2017. The research stages include calibration, calibration curve creation, making standard H2SO4 solutions, measuring the absorbance of the standard solutions, and analyzing the sulfate content in water samples. The research results showed that the sulfate concentration in the Bangkalan, Nginden, Sidoarjo, Keputih, and Kapas Madya well water samples were 14.0817, 27.551, 29.1837, 49.0817, 54.6939 ppm, respectively. Based on the Indonesian Ministry of Health Regulation Permenkes RI No. 32//2017, the maximum sulfate content in water is 400 mg/L, thus it can be concluded that the water samples in those areas have met the clean water standards.
Co-Authors Agustiani, Elly Amali, Hafid Bennani, Kabira Cahyani, Erika Desi Martasari, Amelia Rahayu, Adel Frisca Rahmawati, Affy Milatur Ramadita, Tatya Annur Sampurno, Ade Citra Oktaviana Elok Santoso, Yustia Dwi Fitria Warlinda Eka Triastuti Wintara, Ellen Septia Zahrah, Hanifah Fauziyah