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All Journal Journal of Tropical Biodiversity and Biotechnology Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Febriyanti, Diana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Cryptic Diversity of Barred Mudskippers, Periophthalmus argentilineatus (Valenciennes, 1837), from the Southern Coast of Java and East Lombok, Indonesia inferred by COI Mitochondrial Gene Arisuryanti, Tuty; Aji, Katon Waskito; Herawati, Happy; Sari, Indah Paramita; Rha’ifa, Febrina Amaliya; Febriyanti, Diana; Priyono, Dwi Sendi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 3 (2024): September
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84328

Abstract

The Barred Mudskipper (P. argentilineatus) is an amphibious fish species that displays fully terrestrial behaviour during low tides. Previous studies have indicated the existence of cryptic species of the barred mudskipper, leading to difficulties in taxonomic identification due to similarities in morphological characteristics. Therefore, this study aimed to generate DNA barcodes for Indonesian barred mudskipper populations. We collected ten specimens from Clungup Beach and Kondang Bandung Beach, representing our samples. Additionally, we incorporated 25 previously collected COI sequences from Indonesia into our analysis. The mitochondrial COI gene was amplified using PCR and analysed using various bioinformatics programs. This study provides evidence for the presence of three genetically distinct clades (A, B, and C) within the P. argentilineatus population in Indonesia, with a deep genetic divergence of 2.41% to 6.12%. Clade A showed a high genetic divergence of 5.51-6.12%, suggesting the presence of a cryptic species consistent with previous studies. The high level of haplotype diversity and low nucleotide diversity observed in each clade suggest a population bottleneck followed by a rapid expansion. The lack of geographical separation in the haplotype network analysis indicates that gene flow between populations may have been facilitated by glaciation events in the past. These findings contribute to a better understanding of the biodiversity of the barred mudskipper species in Indonesia and will aid in the accurate identification of cryptic species. This study highlights the importance of using molecular techniques to complement morphological identification in understanding the evolution and diversity of mudskipper fish species. 
Biji Jewawut dan Beras Merah sebagai Media Alternatif Bibit F1 Jamur Tiram dan Jamur Kuping Febriyanti, Diana; Suparti, S
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2019: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.804 KB)

Abstract

Biji jewawut mengandung karbohidrat sebesar 72%- 84,2%, sedangkan kadar karbohidrat beras merah yaitu 77,26 % yang baik untuk pertumbuhan misellium bibit F1 jamur tiram dan jamur kuping. Penelitian ini bertujuan untuk mengetahui pertumbuhan miselium bibit F1 jamur tiram (Pleorotus ostreatus) dan bibit F1 jamur kuping (Auricularia auricula) pada media alternatif beras merah dan biji jewawut. Metode eksperimen menggunakan rancangan acak lengkap (RAL) dengan pola faktorial yang terdiri dari dua faktor yaitu jenis media dan jenis bibit F0 jamur. Parameter yang digunakan dalam penelitian ini yaitu panjang misellium bibit F1 jamur. Analisis data adalah deskriptif kuantitatif. Hasil penelitian diperoleh panjang misellium bibit F1 jamur tiram terpanjang pada media jewawut yaitu 11,40 cm dan untuk pertumbuhan misellium bibit F1 jamur kuping terpanjang pada media beras merah yaitu 7,17 cm. Sedangkan hasil pertumbuhan panjang misellium bibit F1 terendah pada jamur kuping media jewawut yaitu 4,76 cm.
Co-Authors Aji, Katon Waskito Dwi Sendi Priyono Herawati, Happy Rha’ifa, Febrina Amaliya Sari, Indah Paramita Suparti, S Tuty Arisuryanti, Tuty