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APPLICATION OF ETHYLMETHAN SULFONATE MUTATION TO INCREASING THE PRODUCTION OF CUCUMBER (Cucumis sativus L.): APLIKASI MUTASI ETIL METANA SULFONAT UNTUK PENINGKATAN PRODUKSI TANAMAN MENTIMUN (Cucumis sativus L.) Gulo, Alimomon; Sanniwati Saragih, Wismaroh
UPMI Proceeding Series Vol. 1 No. 01 (2023): Ed.01
Publisher : LPPM UPMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55751/ups.v1i01.57

Abstract

Ethyl Methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis sativus L.) seeds were treated with 1% EMS for 12 hours, 24 hours, and 48 hours to optimize EMS mutagenesis and determine the median lethal dose of EMS (1% EMS and 24 hours). This study aims to determine the dose of EMS for cucumber cultivars and report mutant cucumber fruit, flower, vine, and plant sizes induced by EMS. After being treated with 1% EMS for 24 hours, the seeds were grown in a greenhouse for phenotypic investigation. The results showed phenotypic changes, and individual mutants were isolated into six groups, namely: short fruit mutant, long fruit mutant, small flower mutant, large flower mutant, opposite tendril mutant, and group leaf mutant. The mutation frequency is 18.3%. The two selected representatives, the short fruit mutant and the clustered leaf mutant, showed a segregation ratio of 1:3. This ratio is consistent with the classical Mendelian model which shows that the two types of mutants can be controlled by one recessive gene each. The long fruit phenotype was stably inherited, and no segregation was observed in later generations, indicating that this mutant line is probably homozygous.
GROWTH AND PRODUCTION OF SWEET CORN (Zea mays saccharata Sturt.) THROUGH ORGANIC AND INORGANIC FERTILIZATION: GROWTH AND PRODUCTION OF SWEET CORN (Zea mays saccharata Sturt.) THROUGH ORGANIC AND INORGANIC FERTILIZATION Sanniwati Saragih, Wismaroh
UPMI Proceeding Series Vol. 1 No. 01 (2023): Ed.01
Publisher : LPPM UPMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55751/ups.v1i01.85

Abstract

Sweet corn (Zea mays saccharata Sturt.) is a horticultural product that is widely consumed as a vegetable or consumed directly by Indonesian people because it has a sweeter taste than ordinary corn. Corn plants require sufficient nutrients to be able to grow and produce optimally, so fertilization is a determining factor for the success of corn cultivation. This research was carried out in the Experimental Garden of the Faculty of Agriculture, Indonesian Community Development University (UPMI), Medan, from July to September 2022. A factorial randomized block design (RAK) with two treatment factors for organic fertilizer treatment, namely: K0 = Control; K1 = 1 kg/plot; K2 = 1.5 kg/plot; K3 = 2 kg/plot, and inorganic fertilizer treatment, namely: U0 = Control; U1 = 50 g/plot; U2 = 100 g/plot; U3 = 150 g/plot, repeated four times. Parameters observed were (1) plant height (cm), (2) number of leaves (strands), (3) cob length (cm), and (4) cob weight (g). The results of the research on organic fertilizer treatment had no significant effect on the observed parameters. But the application of inorganic fertilizers had a significant effect on plant height but had no effect on the number of leaves observed, cob length, or cob weight of sweet corn. The combination of organic and inorganic fertilizers for growth and production was better than the control treatment or the treatment of organic and inorganic fertilizers.
HOW TO MAKE SUPERIOR SEEDS FOR ORANGE PLANTS CASTURE BY USING TISSUE CULTURE METHOD: CARA PEMBUATAN BIBIT UNGGUL TANAMANAN JERUK KASTURI DENGAN MENGGUNAKAN METODE KULTUR JARINGAN Gulo, Apelius; Gulo, Hiskia; Sanniwati Saragih, Wismaroh
UPMI Proceeding Series Vol. 2 No. 2 (2024): OKTOBER 2024
Publisher : LPPM UPMI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jeruk kasturi (Citrus microcarpa) atau Calamansi fruit merupakan tanaman yang semakin diminati oleh masyarakat sebagai bahan minuman dan pencampur aroma makanan. Produksi jeruk ini masih terbatas pada tanaman pekarangan (Abdullah, 2012). Lamanya masa produktif juga menyebabkan harga jeruk kasturi relatif mahal dikarenakan biaya distribusi dari daerah Sumatra Barat dan Sumatera Utara selaku produsen yang diperhitungkan, sementara permintaan terhadap jeruk kasturi semakin meningkat, sehingga perlu peningkatan ketersediaan bibit yang berkualitas dalam jumlah yang banyak melalui kultur jaringan. Penelitian ini telah dilaksanakan di Laboratorium Kultur Jaringan UPT Benih Tanaman Pangan dan Hortikultura Provinsi Riau, jalan Kaharudin Nasution, Kelurahan Simpang Tiga, Kecamatan Bukit Raya, Kota Pekanbaru. Alat yang digunakan pada penelitian ini adalah laminar air flow cabinet, gelas ukur, gelas piala, petridish, pipet, autoclave, timbangan analitik, erlenmayer, magnetic stirrer, pengaduk kaca, pinset, skarpel, lampu spritus, hand sprayer, pH meter, pisau, botol kultur, kompor gas, labu ukur, tabung reaksi, karet plastik, panci, gunting, alumunium foil, alat tulis dan perlengkapan pencucian yang mendukung kegiatan dalam penelitian keltur jaringan. Berdasarkan hasil penelitian pengaruh pemberian hormon 2,4-D dan BAP terhadap induksi dan pertumbuhan kalus tanaman jeruk kasturi (Citrus microcarpa) didapatkan data Waktu Muncul Kalus. Hasil pengamatan waktu muncul kalus pada Tabel 1 menunjukkan bahwa pemberian kombinasi hormon 2,4-D dan BAP secara deskripsi dari angka yang diperoleh adalah berpengaruh. Pada perlakuan D4B0,5 dan D4B1 merupakan rerata waktu muncul kalus yang paling cepat, yaitu 3,33 HSK. Semakin tinggi kombinasi konsentrasi zat pengatur tumbuh yang ditambahkan dalam medium menyebabkan laju pertumbuhan kalus semakin tinggi, hal ini sesuai menurut Robles-Martinez et al. (2016) penggunaan auksin 2,4- D dapat memacu pertumbuhan kalus, auksin berupa 2,4-D dapat menaikkan tekanan osmotik, meningkat- kan permeabilitas sel terhadap air, menyebabkan pengurangan tekanan pada dinding sel, meningkatkan sintesis protein, meningkatkan plastisitas, dan pengembangan dinding sel.