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All Journal Jurnal Mitra Kesehatan Medical and Health Journal
Rianto, Bayu Dwi
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Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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POTENSI PELARUT ETIL ASETAT PADA EKSTRAKSI FLAVANOID DARI TANAMAN CIPLUKAN (Physalis angulata L.) Sylviningrum, Thianti; Rianto, Bayu Dwi; Agamonanza, Freddy; Ardinas, Shinta Prima
Medical and Health Journal Vol 3 No 2 (2024): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2024.3.2.11407

Abstract

The ciplukan plant (Physalis flavonoi L.) is widely used as a traditional medicine because it contains various bioactive compounds such as flavonoids and physalin. The purpose of this study was to identify and measure the total flavonoid content of ciplukan extracts in various solvents. Ciplukan was extracted by maceration using ethanol, n-hexane and ethyl acetate solvents. Flavonoids were identified by UV-Vis spectrophotometry at 510 nm wavelength. Total flavonoid content was measured based on the calibration curve of quercetin as the standard of comparison. The highest yield of ciplukan extract was obtained from ethanol solvent (18.5%), followed by n-hexane and ethyl acetate. The highest absorbance and total flavonoid content were found in the ethyl acetate extract, which were 0.1162 and 3,709.01 μg/g. Flavonoids from Ciplukan extract were identified most optimally in the semipolar solvent ethyl acetate compared to the polar solvent ethanol and nonpolar n-hexanes. The conclusion of this study is that ethyl acetate extract has the potential to be developed as an herbal medicinal material.
VARIASI VOLUME DARAH TERHADAP DERAJAT AGLUTINASI GOLONGAN DARAH MENGGUNAKAN METODE SLIDE: BLOOD VOLUME VARIATION BASED ON BLOOD AGGLUTINATION LEVELS USING SLIDE METHOD Agamonanza, Freddy; Rianto, Bayu Dwi
Jurnal Mitra Kesehatan Vol. 7 No. 2 (2025): Jurnal Mitra Kesehatan
Publisher : STIKes Mitra Keluarga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47522/jmk.v7i2.372

Abstract

Introduction : Blood typing is one of the important parameters in blood transfusion, patient identification, and various other medical applications. The slide method is one of the easiest, fastest, and most frequently used methods for blood typing. The sensitivity of the slide method is low and can be easily influenced by many factors, making standardization difficult. Inappropriate blood volume can cause vague or difficult-to-read results due to the prozone effect. This study aims to determine the difference in blood volume variations in the degree of agglutination of blood typing using the slide method. Method: The type of research conducted is experimental. The study was conducted at STIKes Bina Cipta Husada Purwokerto in September 2024. The blood volume variations used were 1 µl, 3 µl, and 5 µl. The Kruskal-Wallis test was used to analyze the data.. Result: The results showed a statistically significant difference between blood volume variations and the degree of agglutination with a significance value <0.05. Conclusion: There is a difference in the degree of agglutination based on blood volume variations using the slide method.
Co-Authors Agamonanza, Freddy Ardinas, Shinta Prima Thianti Sylviningrum