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All Journal Tropical Animal Science Journal
Silaen, O. S. M.
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Agriculture, Biological Sciences & Forestry Energy

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Potential of Neuraminidase from Pasteurella multocida for Inhibiting Avian Influenza Virus Subtype H9N2 Replication In Ovo Poetri, O. N.; Nugroho, C. M. H.; Silaen, O. S. M.; Kurnia, R. S.; Krisnamurti, D. G. B.; Indrawati, A.; Hikmah, N.; Hariyadi, I. P. P. K.; Putra, M. A.; Soebandrio, A.
Tropical Animal Science Journal Vol. 46 No. 4 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.4.487

Abstract

In recent decades, neuraminidase/sialidase-based antivirals have been produced to suppress respiratory viral infections, including avian influenza, which relies on sialic acid as the entry point for viruses into cells. While neuraminidase has been extensively studied as an antiviral agent, numerous neuraminidases still have not been evaluated for their antiviral activities. Among these is NanB neuraminidase derived from Pasteurella multocida, which has received limited research attention. This study aimed to assess the potential of NanB neuraminidase in inhibiting H9N2 avian influenza virus infection in ovo. The research commenced with the molecular re-identification of the H9N2 A/Layer/Indonesia/WestJava-04/17 virus isolate, followed by determining the EID50 through Rapid HA test results. The toxicity of NanB neuraminidase was assessed by administering various doses to embryonated chicken eggs (ECE). The antiviral activity of NanB neuraminidase on ECE was evaluated through challenge tests, including treatment before, during, and after the challenge. The assessment involved monitoring the time of embryo death, virus titer through HA test, and viral copy number via RT-qPCR. The results indicated that the H9N2 virus titers capable of infecting 50% of ECE amounted to 108.83 EID50/mL. A dose of 0.258 U/mL of NanB neuraminidase was found to be toxic, leading to embryo mortality after 48 hours of incubation at 37 ℃, while a non-toxic dose was determined to be 0.129 U/mL. The post-challenge treatment group exhibited the most significant reduction in virus titer in ECE. Notably, NanB neuraminidase derived from P. multocida demonstrated the ability to inhibit H9N2 avian influenza virus infection in the ovo model, with the optimal dosage of 0.129 U/mL. The observed decrease in virus titers in the hemagglutination assay and viral copy number assays suggests that NanB neuraminidase holds promise as a potential antiviral candidate for therapeutic approach.
Hematological and Performance Variables of Male Broiler Chickens Fed with Moringa oleifera Extract and Probiotic in Drinking Water Yulianto, A. B.; Lamid, M.; Lokapirnasari, W. P.; Al-Arif, M. A.; Sherasiya, A.; Marbun, T. D.; Khairullah, A. R.; Kurniawan, S. C.; Hasib, A.; Silaen, O. S. M.; Rehman, S.; Ayuti, S. R.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.215

Abstract

Moringa oleifera is a potential plant that can be used to improve immunity, the gut health of broiler chickens, and reduce the number of pathogens in the intestine. Probiotics are non-pathogenic microbes that can balance the microflora in the digestive tract and improve poultry production performance. The objective of this study was to demonstrate the impact of adding probiotic Lactobacillus sp. and M. oleifera extract to broiler feed. In this study, a factorial, completely randomized design was used. This research used 900 animals, which were divided into two factors: the first factor was the dose of M. oleifera (0%, 1%, and 2%) and the second factor was the dose of probiotics (0%, 1 %, and 2%). The treatment was 9 (3 x 3 factorial design), with each consisting of 10 replications and each replication consisting of 10 chickens. M. oleifera extract and probiotics were supplemented in drinking water during the 5-week experimental period. Ad libitum supplies of food and drink were provided. The results revealed that there was an interaction (p<0.05) between the doses of M. oleifera extract and probiotic on leucocytes, monocytes, granulocytes, thrombocytes, and hematocrit when M. oleifera extract and probiotics were added. There was no interaction between the doses of M. oleifera extract and probiotics on the levels of lymphocytes, hemoglobin, and erythrocytes, but all blood profile values were within the normal range. The feed intake, feed conversion ratio, and daily body weight gain showed significant differences (p<0.05) that increased between treatments. It could be concluded that using M. oleifera extract and Lactobacillus sp probiotics as feed additives did not alter the normal blood profile values and could increase the performance of male broiler chickens and income over feed cost (IOFC).
Co-Authors A. Soebandrio Al-Arif, M. A. Ayuti, S. R. Hariyadi, I. P. P. K. Hasib, A. Hikmah, N. Indrawati, A. Khairullah, A. R. Krisnamurti, D. G. B. Kurnia, R. S. Kurniawan, S. C. Lamid, M. Lokapirnasari, W. P. Marbun, T. D. Nugroho, C. M. H. Poetri, O. N. Putra, M. A. Rehman, S. Sherasiya, A. Yulianto, A. B.