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Larvicidal Activity of 96% Ethanol Extract of Arabica Coffee Fruit Peel (Coffea arabica L.) on Mortality of Housefly Larvae (Musca domestica) Lenggar, Benta; Unida, Tazkiah; Putra, Ichsan Luqmana Indra
Journal of Biotechnology and Natural Science Vol. 3 No. 2 (2023): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i2.9870

Abstract

Houseflies (M. domestica) are vectors of disease in humans and animals, so vegetable insecticides are needed that can be used to control M. domestica populations, one of which is Arabica coffee fruit peel extract. The purpose of this study was to determine the effect of giving various concentrations of 96% ethanol extract of Arabica coffee fruit peel on the mortality of M. domestica larvae, as well as calculating the LC50 and LT50 values of M. domestica larval mortality. The research design was a completely randomized design (CRD) using 20 third instar larvae of M. domestica. The concentration variations of Arabica coffee fruit peel extract tested were 0.05%, 0.1%, 0.5%, 1% and 0% as control with 3 replicates using the feeding assay method. Larval mortality was observed for 48 hours (hour 0, 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, and 48) post extract. Data were analyzed by descriptive and inferential analysis using Kruskal-Wallis test and probit analysis of LC50 and LT50. The results showed a significance value >0.05, meaning that there was no significant difference in the mortality of third instar larvae of M. domestica between treatments. The LC50 value obtained was 0.01%. LT50 values at concentrations of 0.05%, 0.1%, 0.5%, and 1% were 274.52 hours, 134.90 hours, 532.20 hours and 0 hours, respectively. The conclusion of this study is that 96% ethanol extract of Arabica coffee fruit peel has no effect on the mortality of M. domestica larvae, the concentration of Arabica coffee fruit peel extract that causes the fastest death of M. domestica larvae is 0.1%, and LT50 at extract concentrations of 0.05%, 0.1%, 0.5% is 274.52 hours, 134.90 hours, and 532.20 hours, at 0% and 1% concentrations have no LT50 value.
Indeks Entomologi dan Faktor Jenis Rumah yang Berkaitan dengan Keberadaan Telur Aedes spp. di Kelurahan Sungai Pinang, Provinsi Jambi: Entomological Index and House Type Factors Related to the Existence of Aedes spp. Eggs in Sungai Pinang Urban Village, Jambi Province Unida, Tazkiah; Satoto, Tri Baskoro Tunggul; Garjito, Triwibowo Ambar; Martono, Edhi
Aspirator Vol 16 No 1 (2025): Jurnal Aspirator Volume 16 Nomor 1 2025
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58623/aspirator.v16i1.117

Abstract

Kelurahan Sungai Pinang memiliki kepadatan penduduk tinggi dan endemis dengue. Keberadaan Aedes spp. sebagai vektor virus dengue diperparah dengan kondisi lingkungan yang kurang terkontrol, seperti banjir yang memperbanyak wadah tergenang sebagai tempat perindukan nyamuk. Variasi tipe rumah juga mempengaruhi populasi Aedes spp., sehingga penelitian mengenai indeks entomologi dan jenis rumah yang mempengaruhi keberadaan telur Aedes spp. perlu dilakukan. Penelitian ini bertujuan untuk mengamati indeks entomologi Aedes spp. di Kelurahan Sungai Pinang (1°59'13.3" S dan 102°02'55.2" E) berupa house index (HI), container index (CI), breteau index (BI), ovitrap index (OI), dan angka bebas jentik (ABJ) di wilayah RT 07 dan RT 18 beserta kaitannya dengan jenis rumah sampling di lokasi penelitian. Penelitian ini menggunakan metode observasional dengan desain cross-sectional. Teknik sampling secara purposive dengan memilih RT dengan kasus dengue tahun 2023 – awal tahun 2024. Data indeks entomologi dianalisis secara univariat, sedangkan analisis korelasi Spearman dilakukan secara bivariat. Hasil penelitian menunjukkan HI sebesar 22,35%, CI 15,22%, dan BI 63,92%. Nilai HI dan CI termasuk dalam kategori densitas sedang, sedangkan BI termasuk kategori tinggi. Angka bebas jentik di RT 07 dan RT 18 adalah 77,65%, masih di bawah target nasional 95%. Ovitrap index mencapai 89,41%, menunjukkan tingkat resiko tinggi. Rumah yang ditemukan telur nyamuk > 10 buah adalah rumah semi-permanen dengan celah di dinding kayu dan atap tanpa plafon. Kelurahan Sungai Pinang masuk dalam kategori berisiko tinggi terjadi penularan dengue.