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All Journal Indonesian Journal of Pharmaceutical Science and Technology Jurnal Jamu Indonesia Indonesian Journal of Jamu
Perdanastuti, Hidayah Sunar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Health Professions Medicine & Pharmacology Other

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Determination of Gamma Oryzanol from Ethanolic Extract of Indonesian Rice Bran (Oryza sativa) Vardhani, Afifah; Perdanastuti, Hidayah Sunar
Indonesian Journal of Pharmaceutical Science and Technology Vol 11, No 1 (2024)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v11i1.46297

Abstract

Rice bran contains gamma oryzanol, a chemical compound mostly consisted of complex ester trans-ferulate with phytosterols1. Gamma oryzanol has a strong antioxidant activity, explained by the free radical scavenging activity. This study was aimed to determine gamma oryzanol from ethanolic extract of rice bran. Quantitative analysis was conducted using HPLC analysis with mixture of methanol:acetonitrile:isopropanol (50%:40%:10%) as mobile phase and Inertsil ODS-3 5 μm as stationary phase. The UV detector wavelength was set to 327 nm, and the flow rate was set to 1 mL/min2. Gamma oryzanol standard and rice bran ethanolic extract 96% was injected into HPLC system. Ethanolic extract of rice bran (Oryza sativa) sample showed four major peaks as those of gamma oryzanol standard and contained 105,981 ppm of gamma oryzanol. As the conclusion, ethanolic extract of rice bran (Oryza sativa) contains gamma oryzanol
Total Phenolic Content in Black Rice (Oryza sativa L. indica) Bran Ethanolic Extract from Two Different Regions in Java, Indonesia Vardhani, Afifah; Anggraini, Nadia Bunga; Jufri, Mahdi; Purwaningsih, Erni; Perdanastuti, Hidayah Sunar
Jurnal Jamu Indonesia Vol. 9 No. 1 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i1.293

Abstract

Rice bran, a rice by-product yielded by the milling process, has recently been recognized as a potential source of natural active constituents. Black rice bran has the highest antioxidant capacity compared to white and brown rice bran. It is correlated with the contribution of phenolic compounds in rice bran. The purpose of this study was to determine the total phenolic content in black rice (Oryza sativa L. indica) bran ethanolic extract from two different regions in Java, Indonesia, using Folin-Ciocalteu Reagent (FCR). Black rice bran samples used in this study were planted in Ciletuh Geopark Sukabumi, West Java (BRBE1), and Karanganyar Regency, Central Java (BRBE2). The parameters observed were total phenolic content, and the result was statistically tested with an unpaired t-test by GraphPad Prism 8.3.0 application. The samples contained 175.48 mg GAE/g extract in BRBE1 and 174.39 mg GAE/g extract in BRBE2. There was no significant difference based on these results. It can be concluded that both samples contained phenolic compounds and statistically, there was no significant difference.
Total Phenolic Content in Black Rice (Oryza sativa L. indica) Bran Ethanolic Extract from Two Different Regions in Java, Indonesia Vardhani, Afifah; Anggraini, Nadia Bunga; Jufri, Mahdi; Purwaningsih, Erni; Perdanastuti, Hidayah Sunar
Jurnal Jamu Indonesia Vol. 9 No. 1 (2024): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v9i1.293

Abstract

Rice bran, a rice by-product yielded by the milling process, has recently been recognized as a potential source of natural active constituents. Black rice bran has the highest antioxidant capacity compared to white and brown rice bran. It is correlated with the contribution of phenolic compounds in rice bran. The purpose of this study was to determine the total phenolic content in black rice (Oryza sativa L. indica) bran ethanolic extract from two different regions in Java, Indonesia, using Folin-Ciocalteu Reagent (FCR). Black rice bran samples used in this study were planted in Ciletuh Geopark Sukabumi, West Java (BRBE1), and Karanganyar Regency, Central Java (BRBE2). The parameters observed were total phenolic content, and the result was statistically tested with an unpaired t-test by GraphPad Prism 8.3.0 application. The samples contained 175.48 mg GAE/g extract in BRBE1 and 174.39 mg GAE/g extract in BRBE2. There was no significant difference based on these results. It can be concluded that both samples contained phenolic compounds and statistically, there was no significant difference.
Co-Authors Anggraini, Nadia Bunga Mahdi Jufri Purwaningsih, Erni Vardhani, Afifah