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All Journal BIOMA : Jurnal Biologi dan Pembelajarannya Indonesian Journal of Ecology and Conservation
Sama, Yusuf
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology

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ISOLASI, KARAKTERISASI, DAN UJI ANTIMIKROBA PADA BAKTERI ASAM LAKTAT DARI USUS Trigona Spp. Sama, Yusuf; Hasgun; Depparinding, Rekianto; Alfiana; Rahman, Auliyah; Manguntungi, Baso; Sari, Arlinda Puspita; Ariandi; Nurmuliayanti, Nurmuliayanti
BIOMA: Jurnal Biologi dan Pembelajarannya Vol 5 No 2 (2023): BIOMA: Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/bioma.v5i2.3120

Abstract

Lebah Trigona spp. merupakan lebah yang tidak mempunyai sengat (stinglees bee) dan memiliki kemampuan memproduksi propolis tinggi sebagai mekanisme pertahanan diri dan sebagai antibiotik terhadap bakteri ataupun cendawan. Pada penelitian in dilakukan proses isolasi bakteri asam laktat (BAL) dari saluran pencernaan Trigona spp. asal Sumbawa yang dilanjutkan dengan karakterisasi BAL, dan uji antimikroba metabolit BAL terhadap bakteri Salmonella thypi dan Staphylococcus aureus. Hasil isolasi diperoleh 20 isolat bakteri, kemudian dilakukan skrining BAL melalui uji katalase dan pewarnaan gram, diperoleh 18 isolat BAL. Kandidat isolat BAL dilkulturkan selama 24 jam, kemudian mengisolasi ekstrak kasar metabolit BAL. Hasil isolasi ekstrak kasar metabolit BAL diujikan ke bakteri S. thypi dan S. aureus selama enam jam menggunakan metode difusi agar, kemudian dilakukan pengukuran zona bening pada jam kedua, keempat dan keenam untuk mengetahui efektivitas daya hambat/antimikroba metabolit BAL. Hasil uji menunjukkan metabolit isolat PRT 9 memiliki kemampuan tertinggi dibandingkan dengan 17 isolat BAL lainnya dalam menghambat pertumbuhan bakteri S. thypi dengan luas zona hambat sebesar 6,7±0,471 mm dan S. aureus sebesar 3,0±0 mm.
Diversity and Distribution of Bunomys (Rodentia: Muridae) Along an Elevational Gradient in Gandang Dewata National Park Sama, Yusuf; Nurdin, Muhammad Rizaldi Trias Jaya Putra; Putera, Alexander Kurniawan Sariyanto; Nurmiati; Pati, Maipa Dia
Indonesian Journal of Ecology and Conservation Vol 2 No 2 (2025): Tropical Ecology and Biodiversity
Publisher : Center for Ecology, Conservation and Ethnobiology Studies, Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/ijec.v2i2.6313

Abstract

This study aimed to determine the distribution and diversity of the genus Bunomys in Gandang Dewata National Park. Fieldwork was conducted in July 2025 using live traps placed across several habitat types and different elevation ranges. Data collection was carried out by installing traps along transect lines at locations suspected to be rat movement pathways. The collected data were analyzed descriptively to identify species and describe their distribution patterns. The results revealed the presence of three species within the genus Bunomys: Bunomys penitus, Bunomys andrewsi, and Bunomys torajae. Each species exhibited different habitat preferences. A total of 16 individuals of B. penitus were found at mid-elevations with primary forest vegetation at elevations ranging from 1,588 to 1,912 m above sea level. Nineteen individuals of B. andrewsi were recorded in secondary forest habitats at elevations between 1,509 and 2,154 m above sea level. Meanwhile, B. torajae was represented by eight individuals found in high mountain areas at elevations of 2,561–2,596 m above sea level. These findings provide important information on the distribution of the genus Bunomys in Sulawesi and highlight the role of Gandang Dewata National Park as an important habitat for endemic wildlife. This study also contributes to enriching the limited biodiversity data on small mammals in the mountainous regions of Sulawesi. Further studies focusing on ecological aspects, behavior, and genetic analysis are recommended to deepen the understanding of population dynamics and phylogenetic relationships within the genus Bunomys
Co-Authors Alexander Kurniawan Sariyanto Putera Alfiana Ariandi Arlinda Puspita Sari Depparinding, Rekianto Hasgun Manguntungi, Baso Muhammad Rizaldi Trias Jaya Putra Nurdin Nurmiati Nurmuliayanti, Nurmuliayanti PATI, MAIPA DIA Rahman, Auliyah