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Test Of Antibacterial Activity Of Ethanol Extract Of Maman Lanang Plant (Cleome Rutidospermae Dc ) Against Gram Positive And Negative Bacteria (Staphylococcus aureus and eschericihia coli) Nurul Furqan, Baiq Repika; Sanjaya, Denny; Almahera, Almahera; Maharani, B Firia
Biota Vol 15 No 2 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i2.486

Abstract

The Maman Lanang plant ( Cleome rutospermae dc ) is a weed plant that is often found in rice fields or grows on community cultivated plants which are usually used as food for people on the outskirts. This research aims to determine the effect of the extract concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) on the inhibition zone for the growth of Staphylococcus aureus and Escherichia coli bacteria, as well as to find out how much the concentration of the extract from the Maman Lanang Plant ( Cleome rutospermae dc ) is close to the inhibitory zone value of the positive control of tetracycline in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria . This research is a laboratory experimental study using a Completely Randomized Design (CRD) using the hole/cup diffusion method with four varying concentrations, namely 10%, 30%, 60% and 90%, and the positive controls used are tetracycline and distilled water as a negative control. The main ingredient used in this research was the Maman Lanang plant ( Cleome rutospermae dc ) which was extracted using the maceration method for three days with 96% ethanol solvent, after that the extract obtained was then subjected to an evaporator to obtain the crude extract then carried out a phytochemical test and after that The antibacterial activity of the crude extract from the Maman Lanang Plant ( Cleome rutospermae dc ) was tested against the bacteria Staphylococcus aureus and Escherichia coli . Then the data obtained was analyzed using the antibacterial ANOVA ( Analysis of variance ) method. The results of this study show that phytochemical test analysis shows the presence of flavonoid compounds in extracts from the Maman Lanang Plant ( Cleome rutospermae dc ). Furthermore, the analysis showed that variations in concentration of the Maman Lanang Plant ( Cleome rutospermae dc ) had an influence on the antibacterial activity of Staphylococcus aureus and Escherichia coli. Where the higher the concentration of the extract, the larger the inhibition zone will be formed. The 90% concentration of the extract showed the largest inhibition zone with a diameter of 23.3 mm.
Identification of the Heavy Metal Lead (Pb) in Red Macro Algae (Gracilia sp) In the waters of Tanjung Luar, East Lombok Almahera, Almahera; Kurniawan, Ahmad Aldi; Maharani, B Firia; Himayanti, Yusfina Firia; Puspita, Novia Dara; Nurul Furqan, Baiq Repika
Biota Vol 17 No 2 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tanjung Luar Village is located in Keruak District, East Lombok Regency, West Nusa Tenggara, is a densely populated body of water human activity. In Tanjung Luar waters, household and ship waste dumped directly into the sea, polluting the local ecosystem. this condition important because people in the area consume a lot of produce marine catches, including red macroalgae (Gracilaria Sp). If Gracilaria Sp. is contaminated with levels of the heavy metal lead (Pb) that exceed threshold of 0.2 mg/kg, can be dangerous to human health impact on the nervous system, urinary system, endocrine system, problems gastrointestinal, and a very high risk of cancer. That thing based on BPOM Regulation No. 23 of 2017 (<0.2 mg/kg) for consumption materials. Therefore pollution and controlling the use of the heavy metal lead (Pb) important, especially in waters that play a supporting role life and food of local communities. The aim of this research is to determine the levels of the heavy metal lead (Pb) contained in the sample red macroalgae (Gracilaria Sp.) in Tanjung Luar Waters. Purposeful research to provide an overview and explanation of the issues discussed. This research uses the wet digestion method to identify heavy metal lead (Pb) of Gracilaria sp in waters Tanjung Luar, East Lombok. Next, levels of the heavy metal lead (Pb) were tested with ICP-OES spectrophotometry. With this method, researchers can identify the concentration of the heavy metal lead (Pb) in Gracilaria Sp. with a high level of accuracy. The results of the study showed that all sampling points of Gracilaria sp. did not exceed the threshold, which was less than 2 mg/kg. where at the sampling location point 1 had Pb concentrations of 0.072 ppm (location A), 0.063 ppm (location B), 0.057 ppm (location D), 0.051 ppm (location E) and 0.046 ppm (location F). for point 2 had Pb concentrations of 0.096 ppm (location A), 0.083 ppm (location B), 0.071 ppm (location C), 0.065 ppm (location E) and 0.059 ppm (location F). for point 3, the Pb concentrations were respectively 0.142 ppm (location A), 0.108 ppm (location C), 0.097 ppm (location D), 0.084 ppm (location E) and 0.070 ppm (location F)