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All Journal Jurnal Farmasi Sains dan Praktis Jurnal Kefarmasian Indonesia
Alviani, Dwi Lutvi
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Chemistry Immunology & microbiology Medicine & Pharmacology Public Health

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MIGRATION INHIBITION ACTIVITY BY METHANOL EXTRACT Hibiscus tiliaceus Linn. ON 4T1 BREAST CANCER Alviani, Dwi Lutvi; Safitri, Erika Indah; Hidayati, Devi Nisa
Jurnal Farmasi Sains dan Praktis Vol 10 No 2 (May-August 2024)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v10i2.9393

Abstract

The prevalence of breast cancer cases in Indonesia is increasing along with the ability of cancer cells to migrate or move from the primary tumor mass and form new colonies elsewhere. The migration of cancer cells has encouraged the develop anticancer drugs from natural ingredients. Waru leaves have been shown to have cytotoxic activity. This study aims to determine the inhibition activity of migration of breast cancer cells 4T1 from methanol extract of waru leaves. Waru leaves methanol extract was obtained using the maceration method. Cytotoxic test of methanol extract of waru leaves (MEWL) was the migration test used in the scratch wound healing method at concentrations 162.5, 325, and 650 μg/mL at 0, 18, 24, and 42 hours after treatment. Analysis of IC50 using linear regression, while large areas were analyzed using Image-J software. The percentage of data closure was analyzed statistically with the Anova Repeated Measure test. All concentrations of Methanol Extract of Waru Leaves had significant inhibition of cell migration (p<0,05) compared to control at each observation time at 0, 18, 24, and 42 hours after treatment. So, MEWL is able to inhibit migration in 4T1 cells
Cytotoxic Activity and Apoptosis by Extract and Ethyl Acetate Fraction of Hibiscus tiliaceus Linn in 4T1 Cell Line Hidayati, Devi Nisa; Safitri, Erika Indah; Surayya, Anis; Alviani, Dwi Lutvi; Putri, Masita Nur Amalia
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 1, FEBRUARY 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Waru leaves are proven to have cytotoxic activity. The activity of waru leaves needs to be developed in a molecular direction to see its apoptotic activity. This study aims to determine the cytotoxic activity of methanol extract (EWL) and ethyl acetate fraction fraction (EAFWL) of Hibiscus tiliaceus Linn as well as the induction of apoptosis in 4T1 cells. The methanol extract of hibiscus leaves was obtained by the maceration method, followed by a fractionation process with n-hexane, ethyl acetate, and water as solvents. Cytotoxic test of EWL was carried out at a concentration of 31.25; 62.5; 125; 250; 500 and 1000 μg/mL while EAFWL was at a concentration of 100; 200; 400; 600 and 100 μg/mL, using MTT assay. Analysis of IC50 values using linear regression. The apoptosis induction test at IC50 and ½ IC50 concentrations using flowcytometry. Data on the percentage of apoptosis were analyzed using One Way Anova statistical analysis. The results were EWL and EAFWL cytotoxic tests against 4T1 had IC50 values of 649 μg/mL and 746 μg/mL. EWL obtained a percentage of total apoptosis at a concentration of 324.5 μg/mL of 48.76%; 649 μg/mL was 67.06% and control cells were 36.16%. EAFWL percentage of total apoptosis at a concentration of 746 μg/mL of 30.8%; 373 μg/mL was 42.13% and control cells were 9.6%. So EWL and EAFWL can induce apoptosis of 4T1 cells.
Co-Authors Devi Nisa Hidayati Erika Indah Safitri Putri, Masita Nur Amalia Surayya, Anis