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All Journal Jurnal Tumbuhan Obat Indonesia Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Indonesian Journal of Chemical Science Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal SOLMA PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Cendekia Eksakta Jurnal Abdi Insani Media Farmasi Indonesia Jurnal Farmasi Sains dan Praktis Jurnal Kefarmasian Indonesia Journal of Pharmascience
Devi Nisa Hidayati
Universitas Wahid Hasyim
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Cytotoxic Activity of Eugenia polyantha Wight Leaves Extract, Purified Extract and Ethyl Acetate Fraction in T47D and Determination of Flavonoid Levels Hidayati, Devi Nisa; Parusiza, Intan Meyta; Fauzizah, Nisa
Indonesian Journal of Chemical Science Vol 11 No 1 (2022)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v11i1.51056

Abstract

Breast cancer is one of the cancers with the highest incidence in women in Indonesia. Old bay leaf extract (Eugenia polyantha Wight) has been proven to contain flavonoids so that it has anticancer activity. The flavonoid compounds can be extracted with ethyl acetate as solvent. The purpose of this study was to determine the cytotoxic activity of extracts, purified extracts, and the ethyl acetate fraction of old bay leaves against T47D breast cancer cells and to investigate their total flavonoid levels. Extraction using maceration method on old Eugenia polyantha Wight powder with methanol solvent, followed by purification and fractionation using ethyl acetate solvent. Cytotoxic activity using the MTT assay method. T47D cells were treated with each test sample with concentration of 31.25; 62.5; 125; 250; 500 and 1000 µg/mL. The absorbance was seen with an ELISA reader and viability cells (%) were calculated to obtain IC50. Determination of flavonoid levels with quercetin and analyzed by UV-Visible spectrophotometry. The result showed that the extract, purified extract, and ethyl acetate fraction of old Eugenia polyantha Wight had a cytotoxic activity with IC50 values of 798.808 g/mL; 593.826 g/mL, and 171.946 g/mL, respectively. Meanwhile, the flavonoid levels were 1.60 mgQE/g, 9.3 mgQE/g, and 5.27 mgQE/g, respectively.
Antibacterial activity of fractions from papaya seeds (Carica papaya L.) extract against Escherichia coli and Salmonella typhi and the contributing compounds Devi Nisa Hidayati; Nurul Hidayati; Esa Evinda; Nur Rahayu Fitriana; Awal Prichatin Kusumadewi
Pharmaciana Vol 9, No 1 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v9i1.12438

Abstract

Papaya (Carica papaya L.) seed has been reported to have antibacterial activity against Escherichia coli and Salmonella typhi. It contains compounds like alkaloids, flavonoids, saponins, and terpenoids. This study aimed to determine the antibacterial activity of fractions from the ethanolic extract of papaya seed against Escherichia coli and Salmonella typhi and identify the specific compounds that contribute to their efficacy. Papaya seeds were extracted through maceration with 70% ethanol solvent, and the extracts were subjected to fractionation with n-hexane, ethyl acetate, and water solvents and followed by a diffusion method to examine the antibacterial activity of both extracts and fractions. Fractions with a series of concentrations were used, namely 10-50% (n-hexane and ethyl acetate fractions) and 100-500% (water fraction). The compounds of the ethanolic extract and its fractions were identified with phytochemical screening. The results showed that the n-hexane and water fractions exhibited antibacterial activity against Escherichia coli when applied at a minimum of 10% and 100% concentration, respectively, but not against Salmonella typhi. Meanwhile, starting from 10% concentration, the ethyl acetate fraction was able to prevent the growth of both Escherichia coli and Salmonella typhi. The compound detection tests revealed that the n-hexane fraction contained alkaloids and terpenoids, the ethyl acetate fraction had alkaloids, flavonoids, and saponins, and the water fraction comprised flavonoids and saponins. 
EFEK EKSTRAK ETANOL BIJI KEDELAI (Glicyne max (L.) Merr) SEBAGAI ANTIKOLESTEROL DAN ANTIOBESITAS PADA TIKUS JANTAN GALUR WISTAR YANG DIINDUKSI MSG DAN IDENTIFIKASI SENYAWA AKTIFNYA Yance Anas; Dewi Pramesti; Siti Wahidatun Nisa; Devi Nisa Hidayati
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2017): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 8 2017
Publisher : Prosiding SNST Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.071 KB)

Abstract

Penelitian ini bertujuan untuk membuktikan efek ekstrak etanol biji kedelai (EEBK) dalam menghambat peningkatan kadar kolesterol total, kenaikan berat badan dan jumlah asupan pakan tikus obesitas yang diinduksi MSG. Tujuan lainnya adalah membandingkan berat organ (jantung, hati, ginjal dan limfa) tikus dengan berat organ tikus kelompok kontrol obesitas dan mengidentifikasi kandungan senyawa aktif flavonoid, saponin dan protein dalam EEBK.  Ekstrak diperoleh dengan cara maserasi menggunakan pelarut etanol 70%. Sediaan uji terdiri dari tiga peringkat dosis EEBK (1,125; 2,250 dan 4,500) g/kgBB/hari, CMC-Na 12,5mL/KgBB/hari (kontrol obesitas), simvastatin 10 mg/KgBB/hari dan Orlistat 10,8 mg/KgBB/hari. Sediaan uji diberikan satu kali sehari selama 14 hari, 30 menit setelah pemberian MSG 100 mg/KgBB/hari. Metode CHOD-PAP digunakan untuk mengukur kadar kolesterol tikus. Sementara itu, data berat badan, asupan pakan dan data berat badan organ diperoleh dengan cara penimbangan. Data dianalisis secara statitstik dengan menggunakan uji beda pada taraf kepercayaan 95%. Hasil penelitian menyimpulkan bahwa EEBK mampu menghambat peningkatan kadar kolesterol total, kenaikan berat badan, asupan pakan tikus jantan galur Wistar yang diinduksi MSG. Berat organ (jantung, hati, ginjal dan limfa) tikus yang mendapatkan perlakuan EEBK 4,500g/kgBB/hari lebih ringan dibandingkan dengan tikus kontrol obesitas. Senyawa aktif golongan flavonoid, saponin serta protein berhasil teridentifikasi dalam EEBK.  Kata kunci: Antiobesitas, ekstrak etanol biji kedelai, kolesterol total, MSG 
ANTITHROMBOCYTOPENIA ACTIVITY OF PEANUT SHELL (Arachis hypogea L.) EXTRACT AND INFUSA ON HEPARIN INDUCED BALB/C MICE risha fillah fithria; Ririn Lispita Wulandari; Devi Nisa Hidayati; Pia Annisa; Nilam Eka Putri
Jurnal Tumbuhan Obat Indonesia Vol 12 No 1 (2019): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (895.35 KB) | DOI: 10.22435/jtoi.v12i1.1266

Abstract

ABSTRACT Certain group of people empirically use peanut shell stew in the treatment of thrombocytopenia due to dengue fever. The active compounds suspected to play a role in the activity of thrombocytopenia from peanut shell are flavonoids. Flavonoids can be extracted using heat and cold methods. This study aims to determine the antithrombocytopenia activity of peanut shell extract and infusa on heparin induced Balb/C mice. This study is an experimental research with randomized matched pre and postest control group design. Peanut shell extraction was done in a cool way by maseration using 70% ethanol solvent, and hot way by infundation using aquadest solvent. Forty-eight Balb/C mice were divided into 8 groups consisting of control group CMC Na 0.5 mL/20gBW/day, control group aquadest 0,5mL/20gBW/day, 3 groups of peanut shell ethanol extract (0.019, 0.038, and 0.076) g/20gBW/day, and 3 groups of peanut shell infusa (0.026, 0.052; 0.104) g/20gBW/day. All treatments were given orally. The decrease of platelet count in Balb/C mice using 26 UI/20gBW subcutan heparin induction. The measurement of the platelet count is performed by taking blood samples in the lateral veins of the tail. Platelet counts data before and after treatment was tested with dependent T-test. Differences in platelet increases across the groups were tested by 2 way anova and continued with a LSD test with 95% confidence level. The results showed that peanut shell ethanol extract had better antitrombocytopenia activity than peanut shell infusa.
Acute Toxicity and Genotoxic Activity of Leunca (Solanum nigrum L.) Herb Ethanolic Extract Rumiyati Rumiyati; Laili Nailul Muna; Devi Nisa Hidayati; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp30-34

Abstract

Previous studies showed that Leunca herb ethanolic extract (LHE) has cytotoxic activity in several cancer cell lines such as HepG2 and HT-29. The extract also demonstrated as potential agent to be developed as co-chemotherapeutic in combination with doxorubicin and cisplatin. The combination is supposed to reduce occurance of cell resistance and  toxicity towards normal cell. In order to verify safety before being applied to human, toxicological and genotoxic evaluation of the extract is important to be done. This research was therefore aimed to investigate acute toxicity and genotoxic activity of LHE. The acute toxicity study was carried out in three groups of 5 mice that was orally administrated by LHE at different doses consisting 300, 2000 and 5000 mg/kgBW. Mortality and sign of toxicity were observed during 7 days. Genotoxic activity was carried out using Mononuclear Polychromatic Erythrosite (MNPCE) assay at a doses range of 250, 500, and 1000 mg/kgBW. Cyclophosphamide (CYP) at the dose of 50 mg/kg was used as positive control. The result showed that LHE did not cause lethal effect on animal model up to doses of 5000 mg/kgBW. However, toxicity signs were found in animal model on day 0-6 of the observation. LHE had lower genotoxic activity compared to cyclophosphamide at the doses of 250, 500, and 1000 mg/kg. The low genotoxic activity and acute toxicity of LHE suggested that the extract might be safe to be used as medicine.Keywords: leunca herb ethanolic extract, genotoxicity, acute toxicity
Mangifera indica L. Leaves Extract Induced Intrinsic Apoptotic Pathway in MCF-7 Cells by Decreasing Bcl-2 Expression and Inducing Bax Expression Fitriasih Fitriasih; Siti Mega Komariyah; Ferry Sandra; Nenni Pratiwi; Devi Nisa Hidayati
Indonesian Journal of Cancer Chemoprevention Vol 10, No 1 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss1pp1-6

Abstract

Extract of Mangifera indica L. has been investigated to have cytotoxic activity against MCF-7 cells.  Current research was conducted to determine the cytotoxic activity of methanol extract of M. indica leaves (MEML) on MCF-7 breast cancer cells and to investigate on the MEML's effect on Bcl-2 and Bax expressions. M. indica leaves were extracted using soxhletation with methanol. The cytotoxic test was perfomed using MTT assay with various MEML concentrations: 15.625, 31.25, 62.5, 125, 250 and 1000 μg/mL. Meanwhile, Bax and Bcl-2 expressions of MCF-7 breast cancer cells were detected with immunocytochemical staining after treatment of MEML with concentrations of ¼ IC50, ½ IC50 and IC50. The results showed that IC50 of cytotoxic activity was 308.12 µg/mL. MEML could influence Bax and Bcl-2 protein expressions. After treatment of MEML, Bcl-2 expression was decreased while Bax expression was increased.Keywords : Mangifera indica L., Cytotoxic, Bax, Bcl-2, MCF-7
Combination of Curcuma (Curcuma xanthorriza Roxb) Rhizome Ethanolic Extract and Awar-Awar (Ficus septica Burm.F) Leaves Ethanolic Extract Increases Cisplatin Cytotoxicity on T47D Breast Cancer Cells through Cell Cycle Modulation Devi Nisa Hidayati; Riris Istighfari Jenie; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp120-125

Abstract

Curcuma (Curcuma xanthorriza Roxb.) and Awar-awar ((Ficus septica Burm.f.) are well known to have anticancer potential especially for breast cancer with low toxicity. This study aims to examine the potential effect of the combination of Curcuma ethanolic extracts (CEE) and awar-awar leaves ethanolic extract (AEE) to increase the efficacy of cisplatin toward T47D breast cancer cells. The combination activity was done using 3 series of concentration, 1/3; 1/6 and 1/12 of IC50, to determine the combination index (CI) of cisplatin, CEE and AEE under MTT assay. The result showed that the combination of 2.5 µM, 5 µg/ml, 1 µg/ml concentrations of  cisplatin, CEE and AEE respectively result in synergistic effect with CI values less than 1. The treatment exhibited the cell accumulation in S phase (27.7%) against T47D breast cancer cells confirmed through cell cycle examination by flow cytometry. These results provided the evidence that the CEE and the AEE can be developed as co-chemotherapeutic agents combined with cisplatin to improve the effectiveness of breast cancer treatment.Keywords : Curcuma xanthorriza Roxb., Ficus septica Burm.f., cisplatin, cell cycle
Decreas of BCL-2 Expression by Ethanol Extract of Ocinum basilicum L. Leaves in Breast Cancer Cells Devi Nisa Hidayati; Fatimatuz Zahroh; Lina Wahyuni; Ibrahim Arifin
Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan Vol 15, No 1 (2021): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran UPN Veteran Jakarta Kerja Sama KNPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33533/jpm.v15i1.2491

Abstract

Ocimum basilicum L has proven to have in vitro cytotoxic activity against breast cancer cells. Pathways that cause cell death can involve one of the proteins, which is BCL-2. This study aims to determine the decrease of BCL-2 protein expressions in breast cancer cells (T47D and MCF-7) tat are treated with the ethanol extract of Ocimum basilicum L. Ocimum basilicum L. was extracted using the maceration method with 70% ethanol solvent. The concentration of ethanol extract of Ocimum basilicum L. used to see the expression of BCL-2 protein in T47D and MCF-7 cells was 199 µg/ml and 388 µg / mL. The observation of BCL-2 protein expression is using immunocytochemical methods of T47D and MCF-7 cancer cells. The results showed that the ethanol extract of Ocimum basilicum L could reduce BCL-2 protein expression in breast cancer cells (T47D and MCF-7) at concentrations of 199 µg/ml and 388 µg/ml, respectively.
Pengujian Aktivitas Antioksidan Ekstrak Dan Fraksi Jantung Pisang Mas (Musa acuminata Colla) Menggunakan Metode DPPH Devi Nisa Hidayati; Ibrahim Arifin; Yuni Antika; Amalina Firdaus; Nur Kussamawari Ardian
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 14 No. 01 Juli 2017
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.906 KB)

Abstract

Jantung pisang mas mengandung senyawa yang dapat menangkap radikal bebas antara lain saponin, fenol, flavonoid. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antioksidan ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas (Musa acuminata Colla) dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, dilanjutkan fraksinasi cair-cair menggunakan pelarut etil asetat dan air. Penentuan aktivitas antioksidan ekstrak etanol dan fraksi air dilakukan pada seri konsentrasi 5, 10, 15, 20, dan 25 μg/ml. Sedangkan untuk fraksi etil asetat digunakan konsentrasi sebesar 4, 8, 12, 16, dan 20 μg/ml. Persentase aktivitas antioksidan diperoleh dari data absorbansi. Nilai IC50 didapatkan dari regresi linier antara konsentrasi larutan seri uji dengan persentase aktivitas antioksidan. Identifikasi senyawa flavonoid dilakukan dengan metode KLT. Hasil penelitian menunjukkan bahwa ekstrak etanol dan fraksi etil asetat ekstrak etanol jantung pisang mas memiliki aktivitas antioksidan kuat, sedangkan fraksi air memiliki aktivitas antioksidan lemah. Nilai IC50 ekstrak etanol, fraksi etil asetat, dan fraksi air jantung pisang mas berturut-turut sebesar 68,742; 56,132; dan 201,151 μg/ml.
AKTIVITAS SITOTOKSIK DAN PENGHAMBATAN MIGRASI SEL KANKER 4T1 DARI EKSTRAK DAUN WARU (Hibiscus tiliaceus Linn.) Devi Nisa Hidayati; Erika Indah Safitri; Dwi Lutvi Alviani; Masita Nur Amalia Putri
Jurnal Tumbuhan Obat Indonesia Vol 15 No 1 (2022): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jtoi.v15i1.5710

Abstract

Kanker payudara merupakan jenis penyakit kanker dengan prevalensi tertinggi di Indonesia. Kegagalan dalam terapi kanker akibat resistensi sel telah mendorong upaya pengembangan obat anti kanker, terutama dari bahan alam. Beberapa penelitian menunjukkan daun waru memiliki aktivitas sitotoksik. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan penghambatan migrasi sel oleh ekstrak daun waru terhadap sel kanker payudara 4T1. Ekstrak daun waru (Hibiscus tiliaceus Linn.) diperoleh dengan metode maserasi. Uji sitotoksisitas ekstrak daun waru dilakukan dengan MTT assay. Uji penghambatan migrasi sel dilakukan dengan metode scratch wound healing. Hasil penelitian menunjukkan nilai IC50 pada sel 4T1 sebesar 892,4 µg/mL. Ekstrak daun waru konsentrasi 892 μg/mL, 446 dan 223 µg/mL mampu menghambat migrasi sel kanker payudara 4T1 berdasarkan persentase penutupan sel pada jam ke 18, 24 dan 42. Dengan demikian, ekstrak daun waru berpotensi untuk dikembangkan lebih lanjut sebagai agen antimigrasi pada sel kanker payudara 4T1.
Co-Authors Alviani, Dwi Lutvi Amalina Firdaus Amaliyah Ayu Nurfitriani Anita Dwi Puspitasari Aqnes Budiarti Aristya Kurniasih Awal Prichatin Kusumadewi Aysah Safitri Chasani, Failasufia Maulida Cicih Sumiarsih Cicih Sumiarsih Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dwi Lutvi Alviani Edy Meiyanto Erika Indah Safitri Esa Evinda Farikha Maharani Fatimatuz Zahroh Fatimatuz Zahroh Fauzizah, Nisa Ferry Sandra Fikriyyah, Shafa Fini Hartanti Fitriasih Fitriasih Fitriasih Fitriasih Gharsina Ghaisani Yumni Hanik Rohmatul Maghfiroh Hartanti, Fini Ibrahim Arifin Ibrahim Arifin Indah Hartati Jenie, Riris Istighfari K. M., Dewi Andini Komariyatun, Siti Laili Nailul Muna Laili Nailul Muna Lalu Ksatria D.S. Lilis Rejeki Lina Wahyuni Lina Wahyuni Lusyana Setiawati Marwan Masita Nur Amalia Putri Mufrod Mufrod Nenni Pratiwi Nenni Pratiwi Nilam Eka Putri Nugroho, Agung NUR AINI Nur Kussamawari Ardian Nur Rahayu Fitriana Nurul Hidayati Parusiza, Intan Meyta Pasha, Salsabilla Putri Buana Pia Annisa Pramesti Dewi Putri, Masita Nur Amalia Rahayu, Putri Rahmatun, Mutia Ririn Lispita Wulandari Riris Istighfari Jenie risha fillah fithria Rumiyati Rumiyati Salsabiila, Iqna Salsabila, Unik Hanifah Siti Mega Komariah Siti Mega Komariyah Siti Nur Chasanah, Siti Nur Siti Nurikha Siti Wahidatun Nisa Sri Susilowati Sufa , Riisyda Faila Sugiyono - Surayya, Anis Syahrul Iqbal Setiawan Tabah Priangkoso Ulya Felasufah Umroh Mahmudah Umroh Mahmudah Yance Anas Yuni Antika