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Nurfalinda, Siti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Comparison of Boiling Methods for Bacterial DNA Isolation Using Waterbath and Heatblock Sirwati, Fadila; Nurfalinda, Siti; Salsabila, Siti; Putri, Dwi
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.328

Abstract

Molecular biology analysis has several advantages over culture approaches, including the ability to identify a wider range of target organisms, increased sensitivity and specificity. Bacterial DNA isolation is a simple solution in molecular biology analysis. Boiling techniques using high temperature heating can damage cell wall permeability. Heating boiling technique can be done by using waterbath, and heat block with different heat transfer mechanism. The results showed the concentration value of samples isolated using headblock was in the range of 77.6 ug/ul - 200.45 ug/ul, while the concentration of samples isolated using waterbath was in the range of 145.575 ug/ul - 288.8 ug/ul. The highest DNA concentration was obtained from using a waterbath. Purity values measured at wavelengths A260/A280 were obtained with a range of purity that was not different.
Genetic Variation Analysis of katG Gene Sequence in Mycobacterium tuberculosis Using RFLP In Silico Nurfalinda, Siti; Aulia, Ony Nattasha; Sirwati, Fadila; Alridho, Rezeki Rival; Achayar, Afifatul
Jurnal Serambi Biologi Vol. 9 No. 1 (2024): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/srmb.v9i1.337

Abstract

Tuberculosis (TB) is an infectious disease that is the leading cause of death in Indonesia. This infection is caused by Mycobacterium tuberculosis. In Indonesia in 2021, there were approximately 824,000 cases of TB. A total of 8,268 TB cases were associated with resistance of M. tuberculosis to anti-tuberculosis drugs (OAT). MDR-TB is a strain that is resistant to two of the most important anti-tuberculosis drugs in first-line TB therapy, namely Rifampicin (RIF) and Isoniazid (INH). The most common mechanism of INH resistance is the katG gene mutation, found (95%) at codon 315. In the katG gene there is a change in the amino acid Ser (AGC) → Thr (ACC). Genetic variation in a population will affect the survival ability of an individual. This study used BamHI restriction enzyme. This study aims to analyze genetic variation in the KatG gene sequence in M. tuberculosis NCBI popset 2363230696 by using RFLP in silico. The results showed that there were genetic variations in the KatG gene sequence and two allelic variations contained in 7 KatG gene sequences using the BamHI restriction enzyme. Key words : M. tuberculosis, BamHI, KatG , Genetic Variation, RFLP in silico.
Co-Authors Achayar, Afifatul Alridho, Rezeki Rival Aulia, Ony Nattasha Dwi Putri, Dwi Salsabila, Siti Sirwati, Fadila