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All Journal Eureka Herba Indonesia Scientific Journal of Dermatology and Venereology
Franklin Shane
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Health Professions Medicine & Pharmacology

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The Role of Moisturizers in Maintaining Skin Barrier Function in a Desert Climate: A Comparative Study in Dubai and a Temperate Region Franklin Shane
Scientific Journal of Dermatology and Venereology Vol. 1 No. 2 (2023): Scientific Journal of Dermatology and Venereology
Publisher : Phlox Institute: Indonesian Medical Research Organization

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59345/sjdv.v1i1.3

Abstract

Introduction: Desert climates present unique challenges to skin health due to low humidity, high temperatures, and intense sun exposure, potentially compromising the skin barrier. Moisturizers play a crucial role in mitigating these effects. This study investigates the impact of moisturizers on skin barrier function in individuals residing in Dubai (desert climate) and a temperate region. Methods: A cross-sectional study was conducted involving 100 participants (50 from Dubai, 50 from a temperate region). Skin barrier function was assessed using transepidermal water loss (TEWL) measurements and stratum corneum hydration levels. Participants' moisturizer usage patterns and perceived skin dryness were recorded. Results: Dubai residents exhibited significantly higher TEWL values and lower stratum corneum hydration levels compared to those in the temperate region, indicating a compromised skin barrier. Regular moisturizer use was associated with significantly improved skin barrier function in both groups, with a more pronounced effect observed in Dubai residents. Conclusion: This study highlights the vulnerability of skin barrier function in desert climates. Regular moisturizer use effectively improves skin barrier function, particularly in arid environments. These findings underscore the importance of moisturizers as a cornerstone of skincare in desert climates.
Curcumin Reverses Cisplatin Resistance in NSCLC via Transcriptional Suppression of ABCB1 and Functional Inhibition of P-glycoprotein: A Mechanistic and Synergistic Analysis Khairiel Anwar; Eduardo Michael Perez; Febria Suryani; Franklin Shane
Eureka Herba Indonesia Vol. 6 No. 1 (2025): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v6i1.131

Abstract

Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related mortality globally. The efficacy of Cisplatin (DDP), a first-line chemotherapeutic, is frequently compromised by multidrug resistance (MDR), primarily driven by the overexpression of the ATP-binding cassette transporter P-glycoprotein (P-gp/ABCB1). Curcumin, a polyphenol with pleiotropic pharmacological effects, has shown potential as a chemosensitizer. This study aimed to rigorously quantify the synergistic interaction between Curcumin and Cisplatin and elucidate whether the reversal of resistance is mediated through functional blockade or genetic suppression of ABCB1. We utilized the human NSCLC cell line A549 and its stable, authenticated DDP-resistant counterpart (A549/DDP). Cytotoxicity was assessed using the CCK-8 assay with strict vehicle controls. Drug synergy was quantified using the Chou-Talalay Combination Index (CI) method. P-gp efflux function was evaluated by Rhodamine 123 (Rh123) accumulation, while apoptosis was analyzed via Annexin V-FITC/PI flow cytometry. The expression levels of P-gp and ABCB1 mRNA were determined by Western blotting and RT-qPCR, adhering to MIQE guidelines. A549/DDP cells exhibited a robust resistance phenotype (Resistance Index: 13.4). Co-treatment with non-toxic concentrations of Curcumin (20 μM) significantly reduced the IC50 of Cisplatin from 56.42 μM to 6.85 μM (p < 0.001), yielding a Reversal Fold of 8.2. The Combination Index was 0.45, indicating strong synergism. Curcumin treatment blocked P-gp-mediated efflux and, critically, downregulated ABCB1 mRNA by 72% and protein expression in a dose-dependent manner. This dual mechanism restored apoptotic sensitivity, increasing rates from 12.5% to 46.8%. In conclusion, curcumin effectively reverses Cisplatin resistance in NSCLC through a dual mechanism: immediate functional inhibition of the P-gp pump and delayed transcriptional repression of ABCB1. These findings support the development of Curcumin-based adjuvant therapies to overcome MDR.
Co-Authors Eduardo Michael Perez Febria Suryani Khairiel Anwar