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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA)
Salma, Qothrunnada Amira
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics

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Isolation and Identification of Acid-Forming Bacteria in Arabica Coffee Fruit (Coffea arabica) During Wet Fermentation Salma, Qothrunnada Amira; Sarkono, Sarkono; Muspiah, Aida
Jurnal Penelitian Pendidikan IPA Vol 10 No 8 (2024): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i8.7335

Abstract

The distinctive flavor and aroma possessed by Arabica coffee are an attraction for coffee enthusiasts. Post-harvest processing of coffee such as coffee fruit fermentation also contributes to the formation of the flavor and aroma of Arabica coffee. In this study, spontaneous fermentation was conducted by adding distilled water, with treatments on fermentation days 4 and 7 aimed at detecting acid-forming bacteria that played a role in the fermentation process. The bacterial isolates were characterized through macroscopic and microscopic observations, physiological, and biochemical tests. Identification of bacterial isolates was performed using the profile matching method based on Bergey’s Manual of Systematic Bacteriology (Holt et al., 1994) and Axellson (2004) until the genus level. The identified bacterial genera were Asaia, Gluconobacter, Neoasaia, Saccharibacter, and Lactobacillus.
Isolation and Identification of Swan (Cygnus olor) Digestive Tract Cellulothic Bacteria to Support Fiber Degradation Rosyidi, Muhammad Subhan Bahruddin; Ali, Muhammad; Wariata, Wayan; Kisworo, Djoko; Khairunnisah; Salma, Qothrunnada Amira; Mursyid, Muhammad Hipzul
Jurnal Penelitian Pendidikan IPA Vol 10 No SpecialIssue (2024): Science Education, Ecotourism, Health Science
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10iSpecialIssue.8516

Abstract

Cellulolytic bacteria are bacteria that produce cellulase enzymes capable of degrading cellulose substrates. This study aimed to isolate and identify as well as measure the cellulase enzyme activity of cellulolytic bacteria isolated from the digestive tract of swans (Cygnus olor) in Mataram. The bacteria were isolated using de Man Rogosa Sharpe (MRS) selective media with the spread plate method. Then, the extracellular enzyme activity test was conducted by growing selected pure isolates on 1% Carboxy Methyl Cellulose (CMC 1%) media, followed by pouring 0.1% congo red solution and rinsing with 1M NaCl solution to determine cellulolytic activity (cellulolytic potential indicated by the appearance of a clear zone around the colony) and measuring the cellulolytic index. The bacterial isolation results obtained 4 isolates with the potential to support fiber degradation in animal feed. The highest cellulolytic index was produced by the isolate coded S1, reaching 34.5 mm, while the lowest cellulolytic index was produced by 2 isolates coded J21 and I2, reaching 18.8 mm.
Co-Authors AIDA MUSPIAH Djoko Kisworo Khairunnisah MUHAMMAD ALI Mursyid, Muhammad Hipzul Rosyidi, Muhammad Subhan Bahruddin Wariata, Wayan