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Isolation and Screening of Cellulolytic Soil Bacteria from Dramaga Forest in Bogor Weridyaningrum, Ratu; Imaningsih, Witiyasti; Fasha, Gusti Nur Aida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.11262

Cellulolytic bacteria can produce cellulase enzymes, which allow them to degrade cellulose into simpler chemicals while using it as a growth substrate. These bacteria have drawn a lot of interest due to their unique properties and potential uses, such as in agriculture and the food industry. Soil is one of the habitats of cellulolytic bacteria. This study aims to determine the potential and screening of cellulolytic bacterial isolates. Cellulolytic bacteria were isolated from soil samples and cultured using selective Carboxyl Methyl Cellulose (CMC) media. Five purified isolates were collected, and their cellulase enzyme activity was qualitatively assessed using the Congo red test. Isolated bacteria code Area Jalan Setapak (AJS1) produced a higher clear zone index and will be used for further testing. Quantitative analysis of isolate AJS1 revealed its highest enzyme activity after ten minutes of incubation, with 0.0049 U/mL unit activity and 0.018 U/mg specific activity. Based on morphological and biochemical characteristics, isolated AJS1 was identified as Corynebacterium spp.

Perbandingan Kualitas Air di Pulau Jawa dan Kalimantan (Review Jurnal) Yuliani, Rida; Imaningsih, Witiyasti
Jurnal Farmasi (Journal of Pharmacy) Vol. 9 No. 1 (2020): Jurnal Farmasi (Journal of Pharmacy), April 2020
Publisher : Jurnal Farmasi (Journal of Pharmacy)

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A review of water quality from the results of research on Java and Kalimantan has been carried out to get an illustration of water contamination level (refill drinking water, PDAM water, and well water) by Coliform bacteria. Data was obtained from secondary sources of research results from several regions in Java and Kalimantan. The results of water quality assessment of the two islands are presented as a percentage. Based on the results, the level of water contamination on Java island is higher than Kalimantan. The average percentage of Coliform contamination in water on Java is 61.42% and only 42.26% in Kalimantan. The high percentage of water contamination by Coliform bacteria in Java is due to the fact that Java has a higher source of contaminants. The existence of Coliform sources was influenced by the population, housing, and organic waste production.

Isolation and Screening of Cellulolytic Soil Bacteria from Dramaga Forest in Bogor Weridyaningrum, Ratu; Imaningsih, Witiyasti; Fasha, Gusti Nur Aida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.11262

Cellulolytic bacteria can produce cellulase enzymes, which allow them to degrade cellulose into simpler chemicals while using it as a growth substrate. These bacteria have drawn a lot of interest due to their unique properties and potential uses, such as in agriculture and the food industry. Soil is one of the habitats of cellulolytic bacteria. This study aims to determine the potential and screening of cellulolytic bacterial isolates. Cellulolytic bacteria were isolated from soil samples and cultured using selective Carboxyl Methyl Cellulose (CMC) media. Five purified isolates were collected, and their cellulase enzyme activity was qualitatively assessed using the Congo red test. Isolated bacteria code Area Jalan Setapak (AJS1) produced a higher clear zone index and will be used for further testing. Quantitative analysis of isolate AJS1 revealed its highest enzyme activity after ten minutes of incubation, with 0.0049 U/mL unit activity and 0.018 U/mg specific activity. Based on morphological and biochemical characteristics, isolated AJS1 was identified as Corynebacterium spp.

The Capability of Consortium Phosphate Solubilizing Bacteria and IAA Producing Fungi on Promoting Elephant Grass Growth Imaningsih, Witiyasti; Kadarsah, Anang; Rusmannurrachmad, Raden Dwi Thriwantho
Jurnal Biodjati Vol 4 No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4284

Â Phosphorus compound in the critical land is relatively low that caused phosphorus is bonded to metals. The isolate BRC-AP and BRC-AK are a phosphate solubilizing bacteria (PSB) indigenous critical land that has the ability to dissolve phosphate. The used of phosphate solubilizing bacteria are able to improve plant growth on critical lands. The aimed of this study was to measure the growth rate of shoot length, root length and biomass of root, and leaf cuttings elephant grass (Pennisetum purpureum cv. King Grass) which inocu-lated with the consortium of phosphate solubilizing bacteria and IAA producing mold. Phosphate dissolving activity is determined by the formation of clear zone in solid Pikovskaya medium, synergism assay between bacteria and fungi observed on SDA medium then proceed testing the ability of bacteria and fungi consortium to promote plant growth. The result showed that the highest of phosphate solubility in-dex was found in isolate BRC-AP (27), addition of the combination of isolate BRC-AP, BRC-AK and Humicola sp. R.Dn have significant effect on shoot growth in the first week and leaf dry weight. The high-est length shoot of cutting elephant grass was found in treatment with the consortium of phosphate solubilizing bacteria and IAA producing mold (BRC-AP + BRC-AK + KP) (58.66 cm). The highest of leaf dry weight was found in treatment with BRC-AP + BRC-AK + KP (5.65 gr). The addition of consortium phosphate solubilizing bacteria and IAA producing fungi from South Kalimantan critical land proved can improve plant growth especially length shoot and leaf dry weight.

INHIBITORY EFFECT OF ULIN WOOD LIQUID SMOKE AND GOGO RICE ENDOPHYTIC FUNGI AGAINST PATHOGEN Pyricularia oryzae Imaningsih, Witiyasti; Mariana; Junaidi, Ahmad Budi; Adventaria, Della
BIOTROPIA Vol. 29 No. 1 (2022): BIOTROPIA Vol. 29 No. 1 April 2022
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2022.29.1.1568

Diseases in rice plants (paddy) caused by microorganisms such as Pyricularia oryzae lead to a decrease in rice production. Therefore, it is essential to find out biological agents for protecting paddy and plants in general, against plant diseases. Liquid smoke and endophytic fungi have been known as biological agents to enhance the protection of plants against disease. The purpose of this study was to determine the ability of liquid smoke, endophytic fungi and the concentrations combinations to suppress the growth of P. oryzae. The results showed that liquid smoke concentrations of 0.17% to 1.75% and endophytic fungi filtrate of 2% to 10% showed significant ability against pathogen P. oryzae. However, the combination of liquid smoke and endophytic fungi filtrate at selected concentrations (0.17% liquid smoke combined with 2% endophytic fungi filtrate and 0.34% liquid smoke combined with 2% endophytic fungi filtrate) showed no significant inhibition percentage against P. oryzae compared to control. In conclusion, this study showed that the respective applications of liquid smoke and endophytic fungi filtrate inhibit the growth of P. oryzae.

PKM EKONOMI HIJAU OLAHAN SAMPAH ORGANIK BERBASIS ECO-ENZYME DI BANK SAMPAH SEKUMPUL MARTAPURA Kadarsah, Anang; Imaningsih, Witiyasti; Fauziah, Yanti
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 8 (2025): Penguatan Ekonomi Masyarakat Berbasis Ekologis untuk Mencapai Keberlanjutan Menuju Ind
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v8i0.2707

Waste management in Sekumpul Urban Village, Martapura, faces serious challenges, where only a quarter of the total 8 tons of daily waste can be properly managed. This is due to limited personnel, low public awareness, and a lack of effective, simple technology implementation. In fact, organic waste has great potential to be processed into economically valuable products such as compost, liquid fertilizer, maggot feed, and eco-enzyme. This community service program (PKM) aims to empower the community of Sekumpul Urban Village by processing organic waste into eco-enzyme-based products with economic value. This creates an integrated solution for both environmental management and improving community welfare. The PKM activities were held at the Sekumpul Waste Bank and involved the integrated participation of students, employees, and waste bank customers. The methods used included socialization, technical training, and program evaluation. Training materials covered waste sorting, compost production, maggot cultivation, and eco-enzyme production to support stingless bee cultivation. The evaluation results showed a remarkable knowledge transformation. Participants' understanding of the green economy concept increased from 7.1% to 100%. Technical knowledge about eco-enzyme production, maggot cultivation, and the stacking bucket method also saw a significant increase. This program successfully created 14 change agents at the community level, who have the potential to develop a green economy based on organic waste processing. These findings highlight the urgency of follow-up programs, such as practical training and continuous assistance, to ensure that the knowledge gained can be implemented into tangible actions.

Exploration Of Potential Endophytic Fungi From Ironwood (Eusideroxylon zwageri Teijsm. & Binn.) As An Antibacterial Agent Raihana, Iffat; Imaningsih, Witiyasti; Wathan, Nashrul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2026): February 2026
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v11i1.11050

This study investigates the endophytic fungi found in ironwood (Eusideroxylon zwageri T et. B), a plant valued by the Kalimantan community for its medicinal uses, especially in treating rheumatism and diabetes. The main goal was to identify these fungi and evaluate their potential antibacterial properties. Samples of ironwood, including leaves and roots, were collected from the CV Nursery at Nusantara Asri, Cempaka Baru, Gunung Kupang, Banjarbaru, South Kalimantan, with coordinates -3.4892255, 114.8907642, 723. Using a series of steps—such as sterilizing samples, isolating fungi, conducting secondary metabolic fermentation, and testing for antimicrobial activity against Staphylococcus aureus and Escherichia coli—11 fungal isolates were successfully obtained from the plant's roots, leaves, and seeds. Five isolates were chosen for further study based on their growth rates, representing various genera including Cladosporium sp., Aspergillus sp., Mucor sp., and Hyphomycetes sp. Although the antimicrobial tests revealed that none of these isolates inhibited the bacteria, the results offer valuable insights into the interactions between these fungi and the bacterial strains tested.

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