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Analisis GC-MS Digesta Serta Konsumsi Pakan dan Berat Badan Tikus (Rattus norvegicus Berkenhout) Setelah Pemberian Fermentasi Tigarun (Crataeva nurvala Buch.-Ham) Fasha, Gusti Nur Aida; Weridyaningrum, Ratu
ORYZA ( JURNAL PENDIDIKAN BIOLOGI ) Vol 14 No 1 (2025): ORYZA: Jurnal Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi STKIP Bima

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33627/oz.v14i1.3145

Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian jus fermentasi tigarun (Crataeva nurvala Buch.-Ham) terhadap konsumsi pakan, berat badan, dan kandungan senyawa volatile dalam saluran pencernaan tikus (Rattus norvegicus Berkenhout). Sebanyak 24 ekor tikus Wistar berusia 12 minggu dibagi menjadi empat kelompok perlakuan: kontrol (K), tikus dengan intervensi tinggi kalori (TK), tikus yang diberikan jus fermentasi tigarun (KJT), dan tikus dengan intervensi tinggi kalori serta jus fermentasi tigarun (TKJT). Penelitian ini menggunakan tikus Wistar jantan yang diberi intervensi nutrien dengan jus fermentasi tigarun, diukur konsumsi pakan dan berat badannya selama 28 hari, dianalisis komposisi senyawa volatile sekumnya dengan GC-MS, dan diuji secara statistik menggunakan One-Way ANOVA serta Uji Duncan untuk menentukan pengaruh fermentasi tigarun. Hasil menunjukkan bahwa konsumsi pakan lebih tinggi pada kelompok K (101,29±30,72 gram) dan KJT (91,25±40,46 gram) dibandingkan TK (61,89±26,93 gram) dan TKJT (55,32±29,61 gram). Namun, kelompok TKJT memiliki berat badan akhir tertinggi (256,67±2,05 gram). Analisis GC-MS menunjukkan kadar asam lemak dodekanoat yang tinggi pada kelompok TKJT dengan persentase relative sebesar 24,22%, yang diduga berperan dalam regulasi mikrobiota saluran cerna. Temuan ini menunjukkan bahwa jus fermentasi tigarun berpotensi meningkatkan efisiensi penyerapan nutrien dan mempengaruhi metabolisme saluran pencernaan.

Isolation and Screening of Cellulolytic Soil Bacteria from Dramaga Forest in Bogor Weridyaningrum, Ratu; Imaningsih, Witiyasti; Fasha, Gusti Nur Aida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.11262

Cellulolytic bacteria can produce cellulase enzymes, which allow them to degrade cellulose into simpler chemicals while using it as a growth substrate. These bacteria have drawn a lot of interest due to their unique properties and potential uses, such as in agriculture and the food industry. Soil is one of the habitats of cellulolytic bacteria. This study aims to determine the potential and screening of cellulolytic bacterial isolates. Cellulolytic bacteria were isolated from soil samples and cultured using selective Carboxyl Methyl Cellulose (CMC) media. Five purified isolates were collected, and their cellulase enzyme activity was qualitatively assessed using the Congo red test. Isolated bacteria code Area Jalan Setapak (AJS1) produced a higher clear zone index and will be used for further testing. Quantitative analysis of isolate AJS1 revealed its highest enzyme activity after ten minutes of incubation, with 0.0049 U/mL unit activity and 0.018 U/mg specific activity. Based on morphological and biochemical characteristics, isolated AJS1 was identified as Corynebacterium spp.

Uji Potensi Probiotik Isolat Lactobacillus plantarum JBSxH.6.4 Asal Fermentasi Tigarun (Crateva nurvala Buch.-Ham.) Secara In Vitro Fasha, Gusti Nur Aida; Nur, Hasrul Satria; Rahmi, Nazarni
Herbal Medicine Journal Vol 8 No 1 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i1.145

Lactobacillus plantarum JBSxH.6.4 isolate was isolated from fermented food of Tigarun (Crateva nurvala Buch.-Ham.), a traditional fermented food from Kalimantan. This study aimed to evaluate its probiotic potential through a series of in vitro tests, including hemolysis analysis, tolerance to acidic pH and bile salt, inhibitory activity against pathogenic bacteria, and interactions with nonpathogenic bacteria. The results showed that Lac. plantarum JBSxH.6.4 exhibited γ-hemolysis, confirming its non-pathogenicity. The isolate survived at pH levels of 2.0, 3.0, and 4.0, demonstrating its acid tolerance. It also exhibited resistance to 0.3% (w/v) bile salt, indicating its ability to survive in the digestive tract. Furthermore, Lac. plantarum JBSxH.6.4 inhibited the growth of Escherichia coli ATCC25922 at 8,08 ± 0,34 mm and Staphylococcus aureus ATCC25923 at 8,95 ± 0,15 mm, suggesting its antimicrobial properties. Additionally, interaction tests indicated that it did not inhibit Lactobacillus acidophilus FNCC0051, supporting its compatibility with beneficial gut microbiota. These findings suggest that Lac. plantarum JBSxH.6.4 meets key probiotic criteria and has potential applications in functional foods and health industries. Further studies, including in vivo evaluations, are recommended to confirm its efficacy and safety for human.

Daya Hambat Bakteri Lactobacillus plantarum JBS4 Asal Fermentasi Tigarun Terhadap Bakteri Patogen Fasha, Gusti Nur Aida; Rahmi, Nazarni; Nur, Hasrul Satria
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Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/jrpi.v2i1.30560

Lactobacillus plantarum JBS4 is a lactic acid bacterium isolated from the fermentation of tigarun (Crataeva nurvala Buch.-Ham) with potential antimicrobial activity. This study aimed to evaluate the inhibitory effect of Lac. plantarum JBS4 against pathogenic bacteria Escherichia coli ATCC25922 and Staphylococcus aureus ATCC25923 in vitro. The inhibition test was conducted using the well diffusion method on NA and MRS agar media. The results showed that Lac. plantarum JBS4 effectively inhibited the growth of E. coli and S. aureus, with inhibition zone diameters of 9.10 ± 0.08 mm and 9.32 ± 0.29 mm, respectively. Furthermore, this isolate did not exhibit inhibitory effects against Lactobacillus acidophilus FNCC0051, a nonpathogenic bacterium. These findings suggest that Lac. plantarum JBS4 has the potential to act as a selective antimicrobial agent against pathogenic bacteria without affecting beneficial microorganisms.

Isolation and Screening of Cellulolytic Soil Bacteria from Dramaga Forest in Bogor Weridyaningrum, Ratu; Imaningsih, Witiyasti; Fasha, Gusti Nur Aida
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.11262

Cellulolytic bacteria can produce cellulase enzymes, which allow them to degrade cellulose into simpler chemicals while using it as a growth substrate. These bacteria have drawn a lot of interest due to their unique properties and potential uses, such as in agriculture and the food industry. Soil is one of the habitats of cellulolytic bacteria. This study aims to determine the potential and screening of cellulolytic bacterial isolates. Cellulolytic bacteria were isolated from soil samples and cultured using selective Carboxyl Methyl Cellulose (CMC) media. Five purified isolates were collected, and their cellulase enzyme activity was qualitatively assessed using the Congo red test. Isolated bacteria code Area Jalan Setapak (AJS1) produced a higher clear zone index and will be used for further testing. Quantitative analysis of isolate AJS1 revealed its highest enzyme activity after ten minutes of incubation, with 0.0049 U/mL unit activity and 0.018 U/mg specific activity. Based on morphological and biochemical characteristics, isolated AJS1 was identified as Corynebacterium spp.

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