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Alizha Aisyah
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Dentistry Health Professions Medicine & Pharmacology Nursing

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Uji Kemurnian Ekstrak Galaktomanan Biji Durian (Durio zibethinus L.) dengan Metode Luff Schoorl Zebua, Nilsya Febrika; Sumardi; Suprianto; Alizha Aisyah
Jurnal Indah Sains dan Klinis Vol 3 No 3 (2022): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v3i3.01

Abstract

Introduction: Galactomannan is found in the endosperm of seeds in the Fabaceae family, for example durian seeds (Durio zibethinus L). Galactomannan is a natural polysaccharide as an additional ingredient. Objective: The research aims to determine the Galactomannan content in Durian seeds isolated by chromatography and to determine the Galactomannan content in Durian seeds using the Luff Schoorl method. Methods: The research was carried out by extracting galactomannan from Durian seeds using distilled water by heating them for 5 minutes. Purification was carried out using paper chromatography with various variations of mobile phases, namely BEW (Butanol: Ethanol: Water), BAW (Butanol: Acetic acid: Water), Glacial acetic acid 10%, Glacial acetic acid 50%, HCl 10%, HCl 20% and Thin layer chromatography with the mobile phase n-Hexane: Ethylacetate at varying ratios of 3:7 and 7:3. Purity testing is carried out using the Luff Scroorl method. Results: Extraction of 500 g of durian seeds produces 1,8% Galactomannan. The levels before and after inversion using the Luff Schoorl method were respectively 8.0131 and 7.0679 mg/mg. Conclusion: Galactomannan can be obtained from extracting durian seeds and its inverse using the Luff Scroorl method.
Co-Authors Nilsya Febrika Zebua, Nilsya Febrika Sumardi Suprianto Suprianto