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Siti Syamsiah
Teknik Kimia, Fakultas Teknik, Universitas Gadjah Mada Jl. Grafika 2, Bulaksumur, Yogyakarta 55281, Indonesia
Chemical Engineering, Chemistry & Bioengineering

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Pengaruh detoksifikasi dan konsentrasi substrat terhadap produksi biohidrogen dari hirolisat ampas tahu Amir Husin; Sarto Sarto; Siti Syamsiah; Imam Prasetyo
Jurnal Rekayasa Proses Vol 8 No 2 (2014): Volume 8, Number 2, 2014
Publisher : Jurnal Rekayasa Proses

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jrekpros.11376

Abstract

The effect of detoxification and substrate concentration on fermentative hydrogen production by mixed cultures was investigated in batch experiments using tofu solid waste (TSW) hydrolysate as substrate. TSW as the by product of tofu processing industry was hydrolyzed using diluted hydrochloric acids as catalyst (0.5% wt HCl, 104°C and 30 minutes). After neutralized by Ca(OH)2 (aq) and then treated by activated carbon for one hour, the hydrolysate was used for biohydrogen production. The experimental results show that, during fermentative hydrogen production under mesophilic condition and initial pH 6.5 were influenced both substrates without/with detoxification. The maximal hydrogen yield of 4.9 mmol/g reducing sugar (RS) were obtained at detoxified substrate consentration of 2 g GT/L. Detoxification has also shown to shortened lag phase of fermentation (l). Adaptation time of microbes during fermentation was reduced from 20 into 13.25 hours for fermentation without/with detoxification respectively at initial substrate concentration of 2 g GT/L.
Co-Authors Amir Husin Imam Prasetyo Sarto Sarto