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All Journal Pharmaciana: Jurnal Kefarmasian
Pramono, Suwidjiyo
Farmasi UGM
Medicine & Pharmacology

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VALIDASI METODE ANALISA PENETAPAN KADAR EPIGALOKATEKIN GALAT DENGAN KROMATOGRAFI CAIR KINERJA TINGGI Sugihartini, Nining; Fudholi, Achmad; Pramono, Suwidjiyo; Sismindari, Sismindari
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.727 KB) | DOI: 10.12928/pharmaciana.v4i1.396

Abstract

High Performance Liquid Chromatography (HPLC) was one of analytical methods commonlyto determine the concentration of epigallocatechin gallate (EGCG) on green tea extract. The methodmust be validated in order to fit to its purpose. The aim of this research was to prove that the usedmethod has selectifity, liniearity, precise, accurate and know limit of detection (LOD) and limit ofquantification (LOQ) is acceptable. The selectifity of analytical method was determined bycalculating the resolution value between two peak. Data from 10 ug/ml and 100 ug/ml with 5replicates would give precition and accuration. Precition was known from CV value and accurationwas known from recovery value in each concentration. Liniearity was known from regression linearbetween concentration and wide area of peak. From regresion linear could calculate LOD and LOQ.Research show that method of analyse have selectificity with Rs= 2,27>1,5; liniearity with r= 0,99;precision with CV 8,74% at concentration 200 µg/ml and 3,74% at concentration 500 µg/ml;accuration with recovery 99,76% at concentration 200 µg/ml and 100,52% at concentration 500 µg/mland the value of LOD is 33,28 ug/ml and LOQ is 110,93 ug/ml.
UJI TOKSISITAS SUBKRONIS EKSTRAK CURCUMA BEBAS MINYAK ATSIRI (ECBA) DITINJAU DARI PARAMETER PROFIL LEUKOSIT PADA TIKUS PUTIH GALUR WISTAR Farida, Verda; Ikawati, Zullies; Pramono, Suwidjiyo
Pharmaciana Vol 4, No 1 (2014): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (106.459 KB) | DOI: 10.12928/pharmaciana.v4i1.398

Abstract

The effect of Volatil Oil of Curcuma Extraxt or Curcumin extract withcentration of essential oil in reducing the levels of cholesterol in the blood has beenECBA as antihyperlipidemic agent is given for a long - term therapeutic purpose. Thisexperiment was performed to investigate the subchronic toxicity of ECBA on leukocytes profile ofWistar rats. ECBA was investigated at dose 15 mg, 120 mg, and 960 mg/200 g body weight for 90days in 40 male Wistar rats. CMC-Na was used for control. The blood was examined for theleukocytes profile on day 0, 45, and 90. The rats were still maintained under experimental conditionswithout giving ECBA until day 105 for the reversibility test. The result showed that the administrationof ECBA for 90 days did not affect leukocytes profile of Wistar rats.
Co-Authors Fudholi, Achmad Nining Sugihartini Sismindari, Sismindari