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All Journal Pharmaciana: Jurnal Kefarmasian
Haryanto, Isnaeni Yudi
Airlangga University
Medicine & Pharmacology

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Penetapan kadar gentamisin dalam sediaan krim dengan kromatografi lapis tipis - densitometri Haryanto, Isnaeni Yudi; Burhanudin, Achmad; poernomo, Achmad toto
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.57 KB) | DOI: 10.12928/pharmaciana.v6i2.3907

Abstract

The used of Gentamicin is still regarded for overcoming infectious diseases. Several preparations of the gentamicin are available in the market as injection, eye or ear drops, and topical dosage forms. Based on the standard method, determination of the gentamicin is carried out by microbiological assay. Several instrumental method have been reported. In this research, Thin Layer Chromatography-Densitometry (TLC-Densitometry) has been validated and used for determining gentamicin in cream dosage form. The TLC was carried out on a Silica gel GF254 using KH2PO4 20% and ninhidrin 2% solution (in ethanol 96%) as eluent and spots visualization respectively. Three spots appeared on the chromatogram having Rf value 0.51, 0.47, and 0.36. All the spots gave maximum absorption at 400 nm. The spot with Rf value of 0.51 was the highest intensity. Limit of Detection (LoD) and Limit of quantitation (LoQ) of the major component was 0.019 and 0.064 respectively. The other validation characteristics met the requirement for determination of gentamicin in the cream dosage form.
Daya hambat kombinasi madu mangga (Mangifera indica)-susu probiotik terhadap pertumbuhan Eschericia coli ATCC 6538 dan Staphylococcus aureus ATCC 8739 Hermawati, Andayanita Hanif; Aryati, Aryati; Haryanto, Isnaeni Yudi
Pharmaciana Vol 6, No 2 (2016): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.437 KB) | DOI: 10.12928/pharmaciana.v6i2.3741

Abstract

Growth inhibitory activity of combination of mango honey-probiotic milk produced by Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) against E. coli and S. aureus has been studied. The fermented milk was prepared by inoculating each probioticin milk at 43ºC and then incubated at room temperature for 24 hours. Diffusion agar technique by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Result of the research showed that pH of fermentation broth of the probiotic milk reached 5. Biomass obtained that calculated by Total Plate Count of L.a and L.c was 1.3 x 107 cfu/mL and 20.7 x 1013 cfu/mL respectively. Inhibition zone diameter ofthe mangohoney-L.c showed an optimum antimicrobial effect against tested bacteria at 1:9 ratio. The MIC value against E. coli and S. Aureus was 15.7 ± 6,1 mm and 13.3 ± 3.0 mm respectively. The activity of mango honey-L. aexhibite dan optimum antimicrobial effect against tested bacteria at 2:8.The MIC value againstE. coli and S. Aureuswas14,7 ± 1,1mm and 13.3 ± 1.3mm respectively.
Co-Authors Achmad Toto Poernomo Aryati Aryati Burhanudin, Achmad Hermawati, Andayanita Hanif