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Effectiveness of Giving Crude Extract of Red Betel Leaf (Piper crocatum) on the Hematology and Histopathology Profile of Goldfish (Cyprinus carpio) Gills Infected with Aeromonas hydrophila Bacteria Muhammad Jusril Arief Pratama; Mohammad Fadjar; Akhsan Fikrillah Paricahya
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.7525

Abstract

The purpose of this study was to analyze the hematology and histopathology of the gills of carp (C. carpio) treated with active compounds of red betel leaf extract (P. crocatum). This research method used a completely randomized design (CRD). The treatments used in this study were doses of 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L, 375 mg/L, negative control (healthy fish), and positive control (positive control), (healthy fish), and positive control (chloramphenicol antibiotic). Results of hematology and histopathology studies the results of hematology and histopathology studies on the gills of carp (C. carpio) after being infected with A. hydropilla bacteria experienced stress, this can be seen from. decreased red blood cells, increased leukocytes, and damage to the gill tissue. Carp returned to normal after being treated with red betel leaf extract. The best dose obtained after treatment was a dose of 300 mg/L. The results of clinical symptom observations showed a change from abnormal to normal conditions in goldfish after being treated. The conclusion is that red betel leaf extract contains dominant compounds in the form of flavonoids and alkaloids which can be useful as a medicine for goldfish infected with A. hydropilla bacteria.
Utilization Ecdysterone Hormone of Krokot (Portulaca oleracea) Extract on Molting Activity in Mud Crabs (Scylla serrate) Gusti Abdillah Ihsan; Maheno Sri Widodo; Abd. Raheem Faqih; Akhsan Fikrillah Paricahya
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.8074

Abstract

The Scylla serrate mangrove crab is one of the leading fisheries commodities in terms of exports, only behind shrimp, tuna and shellfish. Crabs have a fairly high price in foreign markets and most of the mud crabs obtained from wild catches are soft shell crabs. In terms of price, cultivating soft shell crabs is quite profitable, but most cultivators have several problems such as long maintenance periods and inconsistent molting times so that they do not experience business continuity. Applying herbal extracts via injection is an effective method because all the substances that trigger molting directly enter the body. Physiological engineering is needed to speed up the molting phase and get the same molting time. This research aims to identify the ecdysterone content in purslane leaves and determine the optimum dose of purslane leaf extract which can encourage skin molting in mud crabs. This research used a Randomized Block Design (RBD). The in silicons test results show that the compound that has the smallest binding affinity is ecdysone, namely -6.80 kcal/mol. Based on analysis of variance, the best molting percentage occurred in treatment B at 55.50%. The fastest molting period occurred in treatment C, namely on days 4 to 29 after injection. Treatments B and C had the highest survival rate, namely 100%, while in terms of increasing body weight and carapace width, the effect of all treatments was not significantly different. The water quality results for all groups were still within the optimum value, temperature 26.40 – 33.80ºC; pH 6.83 – 7.20; salinity 20.70 – 26.70 ppt and dissolved oxygen 4 – 4.52 ppm