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Widjiati Widjiati
Universitas Airlangga, Indonesia
Nursing

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Number of Glia Cells in the Cerebrum and Cerebellum of Newborn Rats After Administration of Mackerel Fish Oil During Pregnancy Nur Cholila; Widjiati Widjiati; Hermanto Tri Joewono
Jurnal Kebidanan Vol 13, No 1 (2024): February 2024
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jk.13.1.2024.35-40

Abstract

Omega 3 intake during pregnancy can support fetal brain growth and development. Omega 3 plays an important role in making neuron cell membranes and glia and protecting nerve cells. Mackerel is a source of omega 3 which is abundant and easily accessible to the people of Indonesia. The purpose of this study was to analyze the effect of mackerel oil intake on the number of glial cells (astrocytes, oligodendrocytes, and microglia) of the cerebrum and cerebellum of newborn rats. This research was a true experimental design with a post-test only control group. A total of 30 pregnant rats were randomized into 3 groups, namely the control group (K1), mackerel oil (K2), and omega 3 supplements (K3). Each group was given treatment on days 1-17 of pregnancy. On the 18th day, the termination was carried out. 3 newborn rats were taken from each parent and made preparations from brain tissue. Hematoxylin-Eosin test to assess the number of glial cells. The results showed the highest mean±SD number of glial cells (astrocytes, oligodendrocytes, and microglia) in the cerebrum and cerebellum was the highest in the mackerel oil group (174,460±33,777; 21,080±6,937; 11,300±2,090), and (156,280 ±34,980; 22,260±4,302; 11,060±2,383). ANOVA test showed a significant difference in the number of glial cells in the cerebrum and cerebellum between groups with a p-value <0.05. The results of this study can be concluded that the administration of mackerel oil during pregnancy can increase the number of glial cells (astrocytes, oligodendrocytes, and microglia) in the cerebrum and cerebellum of newborn rats.
Effect of Administration of Mackerel Oil During Pregnancy on the Expression of Brain Derived Neurotrophic Factor (BDNF) in the Cerebrum of Newborn Mice Sofiyanti Miftakhurohmah; Widjiati Widjiati; Hermanto Tri Joewono
Jurnal Kebidanan Vol 13, No 1 (2024): February 2024
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26714/jk.13.1.2024.67-72

Abstract

Omega 3 contained in mackerel oil extract is an important constituent of neuron cell walls and the raw material for building fetal brain cells. DHA and EPA in omega 3 regulate BDNF synthesis through a p38-MAPK dependent mechanism. BDNF has a role in regulating cell survival and programming cell death (apoptosis) in the brain. This study aims to determine the effect of giving mackerel oil during pregnancy on BDNF expression in the cerebrum of newborn rats. Posttest research design only control group with true experimental type. A sample of 30 rats with 10 each per group. The first group was the control group (K), the second group was mackerel oil (P1), and the third group was omega 3 supplements (P2). BDNF expression examination methods with immunohistochemistry. The data were analyzed analytically with ANOVA and continued with post hoc LSD. There was a significant difference in the expression of BDNF cerebral newborn rats between groups with a value of p = 0.000 (2.780 ± 0.52 in the control group, 3.670 ± 0.36 in the mackerel oil group, and 3.670 ± 0.45 in the omega 3 supplement group ) . The conclusion of this study was that the group given mackerel oil extract had a higher BDNF cerebral expression than the other groups.
Co-Authors Hermanto Tri Joewono Nur Cholila Sofiyanti Miftakhurohmah