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All Journal Indonesian Journal of Limnology
Riche Hariyati
Diponegoro University, Indonesia
Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Environmental Science

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Environmental DNA Application to Identify Protozoan Community in the Sediment of Balekambang Lake, Dieng, Central Java Shafa Tasya Nabila; Tri Retnaningsih Soeprobowati; Ni Kadek Dita Cahyani; Jumari Jumari; Riche Hariyati; Henk Heijnis
Indonesian Journal of Limnology Vol. 5 No. 1 (2024): Indonesian Journal of Limnology
Publisher : Indonesian Society of Limnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51264/inajl.v5i1.48

Abstract

Lake Balekambang, Dieng, has a high level of sedimentation that can affect water quality, so it is necessary to identify organisms to assess the lake's environmental conditions. The use of eDNA methods in Indonesia is minimal, especially in the Dieng area. This research aims to identify the protozoan communities in sediment samples from Lake Balekambang using the Environmental DNA (eDNA) method to assess the lake ecosystem. This study targeting the 18S rRNA gene for single cell eukaryotes. Data analysis was performed using GALAXY and RStudio. Bioinformatics analysis obtained 51,172 reads divided into 48 Amplicon Sequence Variants (ASVs). All specimens were identified as eukaryotes. Protozoa taxa that can be identified are Tritrichomonadidae, Ophryoscolecidae, Gregarinidae, Cyrtolophosididae, Hexamitidae, Isotrichidae, Oxytrichidae, Vannellidae, Vermamoebidae, and other unidentified Eukaryota taxa. Using the eDNA method this study able to identify the protozoan community found in the sediments of Lake Balekambang, Dieng. Protozoa taxa that are identified are taxa that generally inhabit the rumen of ruminants and the gastrointestinal tract of rodents, and are generally pathogens that can cause disease in humans and animals. New knowledge about Environmental DNA Metabarcoding (eDNA) can support research in identifying organisms. This study shows that the eDNA method utilizing the Next Generation Sequencing (NGS) approach are able to identify the protist more effectively, massively, and quickly compared to conventional methods based on morphological characteristics.
Condition of the Progo River Upstream in Temanggung Regency based on Community Structure and Saprobic Index of Plankton Alvin Afriliandri; Riche Hariyati; Tri Retnaningsih Soeprobowati; Mirza Hanif Al Falah
Indonesian Journal of Limnology Vol. 5 No. 2 (2024): Indonesian Journal of Limnology
Publisher : Indonesian Society of Limnology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51264/inajl.v5i2.79

Abstract

Upstream of the Progo River in Temanggung Regency, it supports the daily lives of the surrounding community. As time goes by, human activities such as land conversion, deforestation, stone mining, agricultural activities and household waste slowly affect the condition of the waters in the Progo River, so it is necessary to monitor water quality. Plankton can indicate changes in water quality because it is responsive to changes in water quality. This research aims to assess the quality of the water environment using community structure and the saprobic index of plankton. Ten sampling stations were selected using the purposive random sampling method. Plankton were collected using a plankton net, water quality measurements were carried out using a Horiba water checker, and testing in the environmental engineering laboratory. As a result, 81 plankton species had an abundance per station of 805-3,981 ind/L. The diversity index (H') indicates a stable ecosystem, the Evenness Index (e) has a fairly even distribution, and the Dominance Index (D) is at all stations with no dominant species. According to PP No. 22 of 2021, the quality of the Progo River Upstream meets environmental quality standard criteria, except for DO and total N (class I). Based on the saprobic index and bioindicator species, it indicates that all stations belong to the ?-Mesosaprobic. Several types of plankton that can be used as bioindicators of water quality include Cladophora glomerata, Tabellaria sp., and zooplankton, which are Lecane sp.
Co-Authors Alvin Afriliandri Henk Heijnis Jumari Jumari Mirza Hanif Al Falah Ni Kadek Dita Cahyani Shafa Tasya Nabila Tri Retnaningsih Soeprobowati Tri Retnaningsih Soeprobowati