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Suryadi, Y
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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VIRULENCE OF Xanthomonas oryzae pv. oryzae AND REACTION OF RICE GENOTYPES TO THE RACES OFTHE PATHOGEN [Vimlensi Xanthomonas oryzae pv. oryzae dan Reaksi Genotipe Padi Terhadap Ras Patogen] Suryadi, Y; Kadir, Triny S
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1973

Abstract

The objective of the trials is to study host-pathogen relationships under green house test and field experiment.The virulence of Xanthomonas oryzae pv. oryzae (XOO) isolates of races III, and VIII is characterized on rice genotypes with different resistance to bacterial blight (BB) disease. The result shows that the multiplication of the bacterial population on the resistant (IR 36) and susceptible (TN-1) rice genotype is almost similar at initial Aage of infection; however, BB symptom expression as indicated by lesion length and area under disease progress curve (AUDPC) are much faster on TN-1 than that on IR 36 genotype. Based on rice field trial in Pusakanagara Expt. St; W. Java; almost all rice genotypes tested shows susceptible (S) to highly susceptible (HS) against XOO race VIII at generative stage observation.
DETEKSI Pseudomonas syringae pv. glycinea (PSG) MENGGUNAKAN ANTIBODIPOLIKLONAL DAN NCM-ELISA Suryadi, Y; Machmud, M
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.045 KB) | DOI: 10.14203/beritabiologi.v8i1.815

Abstract

Soybean bacterial blight is an important disease of the soybean crop. Since resistant cultivars are lacking, the disease is difficult to control, hence early detection and proper diagnosis as well as good knowledge on epiphytotic of the disease are important aspects for successful disease control.A serological technique, particularly the Enzyme-linked Immunosorbent Assay (ELISA) is an effective technique for detection and identification of plant pathogens. The objective of the research was to obtain polyclonal antibodies (PAbs) and use of NCM-ELISA for detection PSG. Soybean plant samples showing symptoms of soybean bacterial blight were collected from the fields and used for antigen sources. Isolation and production of PSG antigen was done using Kings B Agar medium. Immunizations of white New Zealand rabbits were done to produce antibodies to PSG. Yield of PAb-PSG was indicated by antisera titers ranging from 160 to 1280. Intravenous immunization produced more titer than that of intramuscularly. NCM-ELISA was used for detection of PSG from plant samples. It was applicable for detection of PSG from plant samples in relatively short time and limit detection of 10" cfu/ml.
PENGAMATAN INFEKSI JAMUR PATOGEN SERANGGA Metarhizium anisopliae (Metsch. Sorokin) PADA WERENG COKLAT Suryadi, Y; Kadir, Triny S
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.297 KB) | DOI: 10.14203/beritabiologi.v8i6.830

Abstract

Observation on infection of fungus entomopathogen Metarhizium anisopliae on insect brown plant hopper was carried out using scanning electron microscope (SEM).The infection of M. anisopliae on insect bodies was shown by conidia sporulation on divergent chain with conidia size of 1.6 x 7.8 urn. Based upon SEM observation, the results revealed that fungus hyphae of M.anisopliae was found on insect body i.e. on cuticle as well as on segment between abdomen, legs and insect facet eyes.In epizootic condition, the cadavers were shown can act as source of fungus dissemination on healthy insect when environment (temperature and humidity) was suitable for primary infection.
IDENTIFIKASI GALUR Ralstonia solanacearum DENGAN HIBRIDASI SLOT BLOT DNA suryadi, Y
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3447

Abstract

R. solanacearum (RS), the casual agent of bacterial wilt is one of the most destructive pathogen in the tropical and sub tropical areas that affected various economic crops. Since the pathogen is very diverse, it is necessary to identify the variation of RS isolates. The Polymerase Chain Reaction (PCR) was used in this study to amplify 16S rRNA gane. Two DNA primer pairs namely pr#a and pr#b were used as probe to identify thirtyfour strains of Rs respresenting different biovars isolated from various host. The DNA probes were labelled using digoxygenin and deteced by DNA slot blot hybridization. Resutl showed that the DNA probes colud hybridzed specifically with target DNA, hence distinguish variation of Rs isolates. This assay will be of futher used in the strain identification of Rs from wide range of host from various geographic distribution.
Co-Authors Machmud, M Triny S Kadir, Triny S