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Machmud, M
Research Center for Biology-Indonesian Institute of Sciences
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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DETEKSI Pseudomonas syringae pv. glycinea (PSG) MENGGUNAKAN ANTIBODIPOLIKLONAL DAN NCM-ELISA Suryadi, Y; Machmud, M
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (403.045 KB) | DOI: 10.14203/beritabiologi.v8i1.815

Abstract

Soybean bacterial blight is an important disease of the soybean crop. Since resistant cultivars are lacking, the disease is difficult to control, hence early detection and proper diagnosis as well as good knowledge on epiphytotic of the disease are important aspects for successful disease control.A serological technique, particularly the Enzyme-linked Immunosorbent Assay (ELISA) is an effective technique for detection and identification of plant pathogens. The objective of the research was to obtain polyclonal antibodies (PAbs) and use of NCM-ELISA for detection PSG. Soybean plant samples showing symptoms of soybean bacterial blight were collected from the fields and used for antigen sources. Isolation and production of PSG antigen was done using Kings B Agar medium. Immunizations of white New Zealand rabbits were done to produce antibodies to PSG. Yield of PAb-PSG was indicated by antisera titers ranging from 160 to 1280. Intravenous immunization produced more titer than that of intramuscularly. NCM-ELISA was used for detection of PSG from plant samples. It was applicable for detection of PSG from plant samples in relatively short time and limit detection of 10" cfu/ml.
PENDETEKSIAN BAKTERI Raistonia solanacearum, Yabuuchi et al 1995 MENGGUNAKAN TEKNIK REAKSI POLIMERASE BERANTAI DAN PEMBEDAAN STRAIN MENGGUNAKAN TEKNIK HIBRIDISASI DNA Suryadi, Yadi; Machmud, M; Suhendar, MA
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.994 KB) | DOI: 10.14203/beritabiologi.v5i1.1090

Abstract

Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.
Co-Authors Suhendar, MA Suryadi, Y Yadi Suryadi