Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Jurnal Ilmiah Biologi UMA (JIBIOMA) Tropical Health and Medical Research Journal
Novita, Iswiyanti
Unknown Affiliation
Religion Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Studi In Sillico Gen CTX-M15 sebagai Biomarker Escherichia coli ESBL Ayanti, Bio Putri; Adhipurna, Novian; Novita, Iswiyanti; Prihandini, Yustin Ari
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 6, No 2 (2024): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v6i2.5215

Abstract

Escherichia coli is most common nosocomial pathogenic bacteria recognized as the highest cause of ESBL production. Derivatives of CTX-M, especially type CTX-M-15 (blaCTX-M-15) as the dominant ESBL-encoding gene found among E. coli isolates. The most important component in PCR is primer. Primer design can be done by in silico bioinformatics analysis. This study was conducted to produce a primer sequence of E. coli CTX-M-15 gene as a candidate biomarker for E. coli ESBL detection. The method used was in sillico observational using the Primer3Plus application and in silico PCR on candidate primers for the E. coli CTX-M-15 gene. Primers were selected based on criteria including%GC, primer length, Tm (melting temperature), stability, and primer interactions (the presence of dimers and hairpins). The sample used was the nucleotide sequence of the E. coli CTX-M-15 gene strain K-20 plasmid Genbank: GQ330540.1. The results showed that 2 pairs of forward and reverse primer pairs (pairs 1 and pairs 5) were able to amplify the blaCTX-M-15 gene sequence measuring 525bp and 519bp. In silico PCR analysis resulted in 2 pairs of new primer pairs being successfully designed and potentially used as DNA biomarkers from Escherichia coli in early and rapid detection of ESBL infection.
Substitution of Diluent Solution in Semi-Quantitative Examination of C-Reactive Protein Yuliana, Linda; Khairunisya, Siti; Novita, Iswiyanti
Tropical Health and Medical Research Vol. 8 No. 1 (2026): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v8i1.145

Abstract

The C-Reactive Protein (CRP) test using the agglutination method is performed through qualitative and semi-quantitative examinations. However, the diluent solution required for the semi-quantitative stage is not provided in the CRP reagent kit. The absence of this solution requires the laboratory to find an alternative diluent in order for the test to be conducted properly. This study aims to compare the effectiveness of 0.9% NaCl solution from table salt and 0.9% NaCl infusion solution with PBS as a diluent in semi-quantitative CRP examination and to provide an alternative diluent that is cheaper and easier to obtain. The study was conducted in the Immunology Laboratory, Health Polytechnic of the Ministry of Health Banjarmasin, using a true experimental design with a post-test only control group. Experimental groups included 0.9% NaCl from table salt and 0.9% NaCl infusion solution, while the control group used PBS. Nine positive CRP serum samples were used. Friedman test results showed no significant difference between the three diluent types (significance value 0.607 > ? 0.05). It was concluded that 0.9% NaCl solution from infusion and table salt can be used as diluents for semi-quantitative CRP examination. Further research is recommended to test storage stability and application in similar tests, such as Anti-Streptolysin O and Rheumatoid Factor.
Co-Authors Ayanti, Bio Putri Khairunisya, Siti Novian Adhipurna Prihandini, Yustin Ari Yuliana, Linda