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All Journal The Journal of Experimental Life Sciences (JELS) AL KAUNIYAH Idarotuna : Jurnal of Administrative Science
Arifin, Sunanto
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences

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An Assessment of Polar Solvent Extraction on Free Scavenging Activity of Sonneratia caseolaris Iranawati, Feni; Oktavia, Yoan; Kasitowati, Rarasrumdyah; Kartikaningsih, Hartati; Harlyan, Ledhyane Ika; Arifin, Sunanto
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.06

Abstract

Degenerative diseases such as stroke and cancer arise due to oxidative stress by a large number of reactive oxygen species (ROS) in the body. Free radical scavenging (antioxidant) compounds are needed to neutralize the ROS. The natural antioxidant potential of mangrove species Sonneratia caseolaris has been widely explored. However, its extraction is usually carried out with polar solvents such as methanol. Besides being more toxic than ethanol, methanol can cause blindness and death when inhaled in large quantities. Additionally, Indonesia, as the country with the largest Muslim population, requires the use of halal products for food and cosmetics. This study aims to analyze the scavenging activities of S. caseolaris with methanol and distilled water as the polar solvents for extraction. Antioxidant activity was tested with the free radical assay method of DPPH (2,2-diphenyl-1-picrylhydrazyl radicals). The test results were calculated as the IC50 value, a concentration capable of reducing free radicals by 50%. The antioxidant activity test showed that the IC50 values of methanol and distilled water were 18.9 ppm and 36 ppm, respectively. Therefore, both are considered very strong antioxidants. It indicates that distilled water is an acceptable substitute for methanol in the extraction process. Keywords: antioxidant, extraction, mangrove, solvent, Sonneratia.
Model Reproduksi Ikan Berdasarkan Korelasi Panjang, Berat, dan Kematangan Gonad Bahri, Anjas Sasana; Arifin, Sunanto
Idarotuna : Journal of Administrative Science Vol. 5 No. 2 (2024): November
Publisher : Program Study Office Adminstrative of Akademi Komunitas Teknologi Syarifuddin Lumajang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54471/idarotuna.v5i2.61

Abstract

his study aims to analyze the relationship between length-weight, condition factor (K), gonadal maturity stages (GMS), and reproductive strategies of nine fish species captured in the Brantas River, Puri District, Mojokerto. The results indicate that most fish were in GMS II (developing), suggesting that the spawning season was ongoing. Four species—Betok, Kothok, Gatol, and Wader Pari—were found in GMS IV/V, marking the peak or end of the spawning period. The reproductive strategies of these fish species were classified into three main models: seasonal synchronous spawning, batch spawning, and pre-reproductive growth strategies. The findings highlight that the fish community in this river demonstrates flexible reproductive patterns, which serve as an adaptation to the fluctuating environment of the tropical river ecosystem. The contribution of this study lies in the development of a comprehensive model that links key biological parameters—length, weight, condition factor, and gonadal maturity—to the reproductive strategies of riverine fish. This model provides valuable insights into how fish species adapt their reproductive behaviors to the dynamic environmental conditions of tropical river ecosystems. The findings offer practical applications for sustainable fishery management and conservation, enabling more effective monitoring and conservation strategies tailored to specific ecological conditions.
Evaluasi Manajemen Vibrio dalam Budidaya Udang Vannamei (Litopenaeus Vannamei) Arifin, Sunanto; Witriana, Nifa Indah
Idarotuna : Journal of Administrative Science Vol. 6 No. 1 (2025): May
Publisher : Program Study Office Adminstrative of Akademi Komunitas Teknologi Syarifuddin Lumajang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54471/idarotuna.v6i1.123

Abstract

Vibrio bacteria are recognized as major pathogens in shrimp aquaculture, causing significant threats to the health and survival of Litopenaeus vannamei in intensive farming systems. Despite various control measures, the management of Vibrio populations remains a critical challenge for sustainable aquaculture, particularly in tropical environments. This study aims to evaluate the abundance and distribution of Vibrio spp. in both culture water and shrimp body tissues, while simultaneously assessing the effectiveness of management strategies applied at PT Anugrah Tanjung Gumukmas, Jember Regency, Indonesia. Data collection employed a mixed approach, combining microbiological analysis with field observations of aquaculture practices. Water and shrimp tissue samples were taken from multiple ponds, and Vibrio colony-forming units (CFU/ml) were quantified using TCBS agar, followed by species identification to determine dominant strains. The results show that Vibrio concentrations in pond water reached the highest level in pond E22 (4.3 × 10² CFU/ml) and the lowest in other ponds (1.1 × 10¹ CFU/ml), while shrimp tissue samples from pond E14 exhibited the highest bacterial count (3 × 10⁵ CFU/ml). Furthermore, Vibrio populations in shrimp were most abundant in pond E12 (8.9 × 10⁴ CFU/ml), predominantly of Vibrio alginolyticus, exceeding the normal threshold and indicating serious risks to shrimp health. These findings underscore the necessity of continuous bacterial monitoring and adaptive pond management to prevent disease outbreaks. The study contributes to the scientific literature by providing empirical evidence of Vibrio dynamics in intensive vannamei aquaculture under tropical conditions, while also offering practical implications through recommendations for improved water quality monitoring, probiotic application, and bacterial control strategies to support sustainable shrimp farming practices.
DNA Barcoding Primer Design for Species Identification of Skipjack Tuna, Yellowfin Tuna, and Mackerel: Development and Validation of Primers for Genetic Research Iranawati, Feni; Martilla, Denisa Amevia Putri Ananda; Aliviyanti, Dian; Arifin, Sunanto
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 1 (2026): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i1.42706

Abstract

The DNA barcoding technique is primarily utilized to rapidly identify species, particularly when samples are damaged and cannot be identified accurately through morphological methods. This approach utilizes polymerase chain reaction (PCR) technology to amplify DNA fragments from the targeted species, with its success largely due to the design of the primers employed. The cytochrome c oxidase subunit I (COI) gene, a mitochondrial gene, is frequently targeted in DNA barcoding and has been proven effective in distinguishing species. At Pondokdadap Port, over 90% of the fish caught consist of skipjack tuna, yellowfin tuna, and mackerel (T2C). This study aimed at designing silico DNA barcoding primers for these three species. The successful development of these primers may facilitate the documentation and understanding of the genetic diversity of the species under study, which is crucial for efficient and effective fisheries management. The primer design process applied Primer-BLAST software from the NCBI website, followed by additional testing with OligoAnalyzer. The selected primer pairs were the forward primer 5'-GGCCCATGCCTTCGTAATGA-3' and the reverse primer 5'-GCAGGGTCGAAGAAGGTTGT-3'. These primers successfully amplified the DNA of T2C fish, with PCR results indicating that the optimal annealing temperature for these primers was 55 °C
Co-Authors Aliviyanti, Dian Bahri, Anjas Sasana Feni Iranawati, Feni Kartikaningsih, Hartati Kasitowati, Rarasrumdyah Ledhyane Ika Harlyan Martilla, Denisa Amevia Putri Ananda Oktavia, Yoan Witriana, Nifa Indah