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Hardarani, Novia
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Agriculture, Biological Sciences & Forestry

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Respon Pisang Sulindang (Musa Paradisiaca L.) terhadap Formulasi Media MS dengan Sitokinin Wismantia, Welly; Rodinah, Rodinah; Hardarani, Novia
Agroekotek View Vol 6, No 2 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/agtview.v6i2.3248

Abstract

The availability of banana seeds, especially Sulindang bananas as a fruit commodity in South Kalimantan, cannot be met on a large scale. The problem is that Sulindang banana seeds are still produced conventionally that produce small amounts of weeds. The solution that can be done to get maximum seed yield is by tissue culture. The research aims to determine the influence of interaction between BAP and kinetin on the growth of banana weevil explant Sulindang. This study used RAL two factors in the form of kinetin concentration and BAP concentration, consisting of 4 levels of kinetin; k0 = 0 mg l-1 kinetin; k1 = 1 mg l-1 kinetin; k2 = 2 mg l-1 kinetin; k3 = 3 mg l-1 kinetin and consists of 4 levels of BAP; b0 = 0 mg l-1 BAP; b1 = 2 mg l-1 BAP; b2 = 2.5 mg l-1 BAP; b3 = 3 mg l-1 BAP. The observed variables are changes in the morphology of the explant, the time the buds appear, the number of buds, the number of leaves, the percentage of living explants, the percentage of explants of contamination and the percentage of browning. The results of this study showed that there was no interaction between various concentrations of BAP and kinetin with banana explant Sulindang in the in vitro growth response in MS media.  The BAP concentration did not affect the growth of the Sulindang Alas banana weevil explants. Single factor kinetin 2 mg l-1 was able to give the best results on the percentage of live explants at week 3 and week 6, respectively 96.88% and 93.75%. Also gives the smallest percentage of contamination at week 3 of 3.13% and week 4 to week 6, respectively - 6.25%.
Pengaruh Kolkisin terhadap Keragaman Fenotipe secara In Vitro pada Tanaman Stevia (Stevia rebaudiana Bertoni) Novitasari, Desty; Nisa, Chatimatun; Hardarani, Novia
Agroekotek View Vol 6, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/agtview.v6i3.4789

Abstract

Stevia (Stevia rebaudiana Bertoni) is a plant that produce low-calorie natural sweeteners that can be used as a substitute for sugar cane for people with Diabetes Mellitus (DM) and obesity. Improvements to the properties of stevia in order to produce low-calorie natural sweeteners are mostly carried out in the laboratory using in vitro techniques. The media used were Murashige and Skoog with the addition of IAA dan BAP. This study aims to determine the phenotypic diversity in stevia with the addition of various concentrations and duration of immersion of the mutagen in the form of colchicine and to find the best interaction treatment used. The study was in the form of a two-factor randomized block design, the first factor being various concentrations of colchicine, namely  k0 = 0% as control, k1 = 0,03% and k2 = 0,05%. The second factor was the duration of colchicine immersion, namely  t1 = 24 hours and t2 = 48 hours. The result of this study indicate that the interaction of concentration and duration of colchicine immersion has a significant effect on the percentage of contamination with a concentration of 0,05% treatment and 24 hours immersion as the best contamination percentage treatment of 12,50%. The highest percentage of live explants was treated with a 24 hours immersion period of 8,33% and the lowest percentage was found in the 48 hour immersion treatment at 0%. The lowest percentage of browning was in the 0,05% colchicine concentration treatment at 58,33% and the highest percentage was found in the 0,03% concentration colchicine treatment at 93,75%.
Co-Authors Chatimatun Nisa Novitasari, Desty Rodinah Rodinah Wismantia, Welly