Azkarahman, Aldyon Restu
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Comparison of the Bioactive Properties of Honey Proteins from Floral Sources in Indonesia Jaya, Firman; Evanuarini, Herly; Susanto, Edy; Azkarahman, Aldyon Restu
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 1 (2024): April 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.01.09

Abstract

The diversity of plant species in Indonesia has resulted in a wide variety of floral honey. The antioxidant activities of these Indonesian honeys have not been determined. Ten types of honey from indigenous Indonesian sources (9 monofloral; 1 heterofloral) were evaluated for their antioxidant activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method. The Folin-Ciocalteu test was used to determine the total phenolic content, and an aluminum chloride colorimetric assay was used to determine the total flavonoid content. SDS–PAGE was used to determine the molecular weights of the honey proteins. The mean antioxidant activity of the honey samples, defined as the IC50 of DPPH, ranged from 5.46 to 34.42. The mean total phenol content ranged from 27.57 to 53.85 mg GAE/100 g, and the mean flavonoid content ranged from 3.58–15.67 mg CE/100 g. A protein of ~60 kDa was detected in all honey varieties. A positive correlation was observed between antioxidant activity, total phenols, and total flavonoids, indicating that their phenolic and flavonoid components confer the antioxidant activity of these honey varieties. To the best of our knowledge, indigenous Indonesian honey, especially monofloral Calliandra honey, has shown greater antioxidant activity than other kinds of honey worldwide.
Sensory and Chemical Evaluation of Jelly Candy with Honey Powder Substitution Amertaningtyas, Dedes; Amalia, Alvina Wahyu; Evanuarini, Herly; Susilo, Agus; Jaya, Firman; Widati, Aris Sri; Azkarahman, Aldyon Restu; Masyithoh, Dewi
Jurnal Ilmu dan Teknologi Hasil Ternak Vol. 20 No. 3 (2025)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2025.020.03.2

Abstract

Jelly candy is a type of soft candy made from fruit extract, sugar, and gelatin. The high sugar content may have adverse health effects. Therefore, honey powder has potential as a partial sugar substitute as well as a functional ingredient. The aim of this study was to determine the best percentage of honey powder substitution to replace part of the sugar in milk jelly candy. The main raw material was cow’s milk with honey powder as a sugar (sucrose) substitute. The study used a Completely Randomized Design (CRD) with four treatments and four replications. The treatments were T0 (25% sugar without honey powder), T1 (19% sugar + 6% honey powder), T2 (12.5% sugar + 12.5% honey powder), and T3 (6% sugar + 19% honey powder). The results showed that partial substitution of sugar with honey powder had a highly significant effect (p < 0.01) on reducing sugar (5.56-11.43%), antioxidant (90.16-116.73 ?g/mL), and color of sensory evaluation (3.58-4.05), as well as a significant effect (p < 0.05) on protein content (11.51-12.50%), taste (3.78-4.18), aroma (3.53-3.80), and overall acceptance (3.50-3.90), but no significant effect (p > 0.05) on texture (3.43-4.18). It can be concluded that substituting part of the sugar with honey powder in milk jelly candy affects the chemical properties. However, based on the evaluation, the control treatment with 25% sugar and no honey powder still be the most preferred product by panelists.