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All Journal Asian Journal of Aquatic Sciences
Luskityowati, Iesje
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Agriculture, Biological Sciences & Forestry

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HISTOPATHOLOGY OF KIDNEY AND INTESTINE OF TILAPIA (Oreochromis niloticus) FED WITH HERB FERMENTED FEED AFTER CHALLENGE OF Streptococcus agalactiae Al Furqon, Abdullah; Riauwaty, Morina; Luskityowati, Iesje
Asian Journal of Aquatic Sciences Vol. 6 No. 2 (2023): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The natural ingredients' potential as immunostimulants is a plant Curcuma and Aromatic ginger. This research was conducted from March to June 2022 in the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine, Universitas Riau. Histological preparations were carried out at the Bukittinggi Veterinary Center (BVet). This research aimed to know the structure of the Kidneys and Intestines of tilapia by adding herb-fermented feed to prevent Streptococcus agalactiae infection. This research was an experimental method using a Completely Randomized Design (CRD) with 4 treatments and 3 replications. This treatment of the study was; NC (were not immersed and infected), PC (were not immersed in fermented herbs solution but infected of S.agalactiae), T1 (Giving 100 mL/kg fermented herbs), T2 (Giving 125 mL/kg fermented herbs), T3 (Giving 150 mL/kg fermented herbs). The fish used were 6 - 7 cm in size and reared with a stocking density of 1 fish/3 L of water and kept for 46 days. Fish were challenged with S.agalactiae bacteria with a density of 108 CFU/mL at a dose of 0.1 mL/head intramuscularly on day 32, after which the fish were reared again until day 46. Changes that occur in the kidneys of fish are hemorrhage, hypertrophy, and necrosis, while in the intestines there are histological changes in the form of haemorrhage, edema, vacuole degeneration, and necrosis. The results show that the feeding containing the herbal medicine of Curcuma and Aromatic ginger fermented affected the improvement of the histological changes in the kidneys and intestines of tilapia.
IDENTIFICATION OF LACTIC ACID BACTERIA (BAL) FROM THE INTESTINES OF Pangasianodon hypophthalmus THAT WAS FEEDED BY FERMENTED HERBAL AND THE ABILITY TO INHIBIT Aeromonas hydrophila BACTERIA mASTUTI, Rini; Syawal, Henni; Luskityowati, Iesje
Asian Journal of Aquatic Sciences Vol. 5 No. 3 (2022): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Lactic acid bacteria can improve digestibility because they can be absorbed directly by the body. The purpose of this research was to obtain lactic acid bacteria types from the intestines of striped catfish (Pangasionodon hypophthalmus) that had been fed fermented herbal feed. This research was conducted from June 2019 to April 2020. The treatment used in this research was with different doses and stocking densities. The test was carried out using the disc diffusion method or the Kirby-Bauer method, which was done by measuring the inhibition zone around the paper disc. Identification of LAB was carried out by phenotypic and genotypic. The results showed that as many as four of the selected isolates were able to inhibit the growth activity of the pathogenic bacteria Aeromonas hydrophila. Isolates with code K had an inhibition zone of 6.7 mm against A.hydrophila, an inhibition zone of 7.5 mm for P1D2 isolates, P2D1 isolates had an inhibition zone of 7.0 mm, P2D2 isolates had an inhibition zone of 7.8 mm. Types of LAB that were identified phenotypically and genotypically include Lactobacillus casei, L.plantarum, and L. acidhopilus
HEMATOLOGY OF Pangasianodon hypophthalmus THAT WERE FED WITH CONTAINING FERMENTED RED GINGER (Zingiber officinale var. Rubrum) TO PREVENT THE Motile Aeromonas Septicaemia DISEASE Pulungan, Listi Agustina; Riauwaty, Morina; Luskityowati, Iesje
Asian Journal of Aquatic Sciences Vol. 5 No. 2 (2022): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Red ginger is a natural ingredient that has antibacterial compounds. This research was conducted from June to August 2021 at the Fish Disease and Parasite Laboratory, Department of Aquaculture, Faculty of Fisheries and Marine, Universitas Riau, Pekanbaru. The aim of this study was to obtain the best dose of fermented red ginger added to the feed on the hematology of striped catfish. The method used is an experimental method using one factor Completely Randomized (CRD) with five treatment levels and three replications. The treatments applied were Kn: negative control (no treatments), Kp: positive control (no treatments and infected with A. hydrophila), P1 (150 mL/kg of feed and tested against A.hydrophila, P2 (175 mL/kg of feed), P3 (200 mL/kg of feed). On the 32nd day, the fish were infekted with A. hydrophila at a density of 108 CFU/mL of 0.1 mL/fish intramuscularly, then reared up to 46 days. The results shown that there was an effect of adding fermented red ginger in the feed on the hematology of striped catfish before and after the challenge test with A. hydrophila. The best dose was P2 (175 mL/kg) with a total erythrocyte range of 2.31 x 106 cells/mm3, hematocrit was 39.33%, hemoglobin was 10.40 g/dL, total leukocytes was 11.12 x 104 cells/mm3, survival rate 93.33%, absolute weight growth 19.34 g and water quality during temperature ranged from 28.3-29.2oC, pH 6-7, DO 4.7-5.5 mg/L, and NH3 0.002-0.003 mg/L.
Co-Authors Al Furqon, Abdullah Henni Syawal Pulungan, Listi Agustina Rini Mastuti