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All Journal Journal of Natural Sciences and Mathematics Research Walisongo Journal of Chemistry
Widodo, Naufaldi Bani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Physics

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Antibacterial Activity from Fraction of Liquid-Liquid Extraction in Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) Alighiri, Dante; Firnanda, Indah Putri; Abdullah, Ravely Adhitya; Widodo, Naufaldi Bani; Drastisianti, Apriliana
Walisongo Journal of Chemistry Vol. 7 No. 2 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i2.23333

Abstract

The rhizome of Temulawak (Curcuma xanthorrhiza Roxb) has been traditionally used to treat various ailments due to its rich content of curcumin, demethoxycurcumin, and bisdemethoxycurcumin. These compounds exhibit notable antioxidant and antibacterial properties. This study aimed to evaluate the antibacterial efficacy of Temulawak rhizome extracts in n-hexane, ethyl acetate, and methanol, focusing on Escherichia coli and Staphylococcus aureus. The extraction process involved successive maceration with each solvent. The antibacterial activity was tested using well diffusion and microdilution methods, determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phytochemical screening revealed the presence of flavonoids, alkaloids, phenolics, steroids, and triterpenoids in the extracts. The results indicated that the n-hexane and ethyl acetate fractions exhibited intense antibacterial activity against Staphylococcus aureus, while all fractions showed potent activity against Escherichia coli. The MIC values ranged from 39.0625 to 625 ppm for Staphylococcus aureus and 39.0625 to 78.125 ppm for Escherichia coli, with MBC values generally higher than MIC values. These findings highlight the potential of Temulawak rhizome extracts as effective antibacterial agents.
Antibacterial Activity from Fraction of Liquid-Liquid Extraction in Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) Alighiri, Dante; Firnanda, Indah Putri; Abdullah, Ravely Adhitya; Widodo, Naufaldi Bani; Drastisianti, Apriliana
Walisongo Journal of Chemistry Vol. 7 No. 2 (2024): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology UIN Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v7i2.23333

Abstract

The rhizome of Temulawak (Curcuma xanthorrhiza Roxb) has been traditionally used to treat various ailments due to its rich content of curcumin, demethoxycurcumin, and bisdemethoxycurcumin. These compounds exhibit notable antioxidant and antibacterial properties. This study aimed to evaluate the antibacterial efficacy of Temulawak rhizome extracts in n-hexane, ethyl acetate, and methanol, focusing on Escherichia coli and Staphylococcus aureus. The extraction process involved successive maceration with each solvent. The antibacterial activity was tested using well diffusion and microdilution methods, determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Phytochemical screening revealed the presence of flavonoids, alkaloids, phenolics, steroids, and triterpenoids in the extracts. The results indicated that the n-hexane and ethyl acetate fractions exhibited intense antibacterial activity against Staphylococcus aureus, while all fractions showed potent activity against Escherichia coli. The MIC values ranged from 39.0625 to 625 ppm for Staphylococcus aureus and 39.0625 to 78.125 ppm for Escherichia coli, with MBC values generally higher than MIC values. These findings highlight the potential of Temulawak rhizome extracts as effective antibacterial agents.
Risk analysis of air quality for parameters NO2, SO2, NH3, and Ox from the area around fertilizer industries in Indonesia Alighiri, Dante; Widodo, Naufaldi Bani; Abdullah, Ravely Adhitya; Firnanda, Indah Putri; Drastisianti, Apriliana
Journal of Natural Sciences and Mathematics Research Vol. 11 No. 1 (2025): June
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.v11i1.23331

Abstract

Industrial zones with intensive human activities often experience air-quality degradation, particularly in areas surrounding fertilizer production facilities. This study analyzes the concentrations of nitrogen dioxide (NO₂), sulfur dioxide (SO₂), ammonia (NH₃), and oxidants (Ox) in the ambient air around fertilizer industries in Indonesia and evaluates their potential non-carcinogenic health risks. Air sampling was conducted using an InScienPro US-1012 impinger equipped with selective absorbent solutions. Pollutant concentrations were quantified using UV-Vis spectrophotometry based on the Griess–Saltzman method for NO₂, the pararosaniline method for SO₂, the indophenol method for NH₃, and the neutral buffer potassium iodide method for Ox. Concentrations were compared with national ambient air-quality standards. To strengthen the assessment, a quantitative health-risk evaluation was incorporated using the Hazard Quotient (HQ) and Hazard Index (HI), allowing for the interpretation of long-term exposure impacts. Results show that most pollutant concentrations were below regulatory limits; however, SO₂ levels at one sampling point and NH₃ levels near the production zone were elevated. The HQ–HI analysis revealed that SO₂ at the office-area sampling point (HQ = 0.794) and NH₃ inside the processing plant (HQ = 2.238) were the dominant contributors to cumulative exposure, with HI values exceeding 1 at both locations. These findings indicate potential non-carcinogenic risks for individuals chronically exposed in areas closest to emission sources. Overall, this study highlights the importance of continuous monitoring and risk-based evaluation to identify localised hotspots, inform mitigation strategies, and enhance emission control practices in fertiliser-industrial environments.
Co-Authors Abdullah, Ravely Adhitya Alighiri, Dante Drastisianti, Apriliana Firnanda, Indah Putri