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All Journal International Journal of Cell and Biomedical Science
Kustyah, Azizah Retno
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Hypoxia Precondition Enhance the Therapeutic Effects of Mesenchymal Stem Cells via regulating TGF-β1 and IL-10 serial expression in Skin Excision Rat Models Kustyah, Azizah Retno; Fatimah, Nandiah; Rizkiyani, Elytia Mutia; Ramadhanti, Olifiarsy Wiet; Istiqomah, Dyah Ayu Fitri; Hidayah, Nurul; Bhirau Wilaksono
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.9

Abstract

Background: The skin excisional wound healing process involves an intricate-regulated series of cellular responses to reverse the formation of skin tissue integrity. This process requires paracrine communication involving anti-inflammatory cytokines and growth factors, especially interleukin 10 (IL-10) and TGF-β. On the other hand, hypoxic preconditioned mesenchymal stem cells (Hypoxia-MSCs) have been acknowledged to enrich IL-10 and TGF- β secretion contributing to accelerated wound healing compared to normal preconditioned mesenchymal stem cells (Normoxia-MSCs). Objective: This study aimed to compare Hypoxia-MSCs and Normoxia-MSCs in integrating the serial expression of IL-10 and TGF-β associated with improved collagen density in animal models of excision wounds. Methods: Thirty-six male Wistar rats with excision wounds were made as animal models using the 6 mm biopsy method. The rats were randomly divided into four groups consisting of four treatment groups: N-MSCs 1x106, H-MSCs 1x106, Control (PBS treatment), and Sham (untreated or healthy mice). The treatments were administered 2 times intraperitoneally on day 0. Skin tissue was collected on days 12, 18, and 24 post-injections. IL-10 dan TGF-β expressions were examined by qPCR. Results: This study showed that there was a significant increase in IL-10 and TGF-β after Hypoxia-MSCs and Normoxia-MSCs treatment compared to the Control group. Conclusion: Hypoxia-MSCs can improve the serial expression of IL-10 which leads to wound repair of the mouse model of excision wound. These results suggest that a hypoxic environment can enhance the therapeutic effect of MSCs.
The Effect of Low Dosage MSC-Conditioned Medium on Urea Levels in Acute Renal Failure Istania, Rizqi Windhu Sri; Kustyah, Azizah Retno; Sa'dyah, Nur Anna Chalimah
International Journal of Cell and Biomedical Science Vol 1 No 2 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i2.17

Abstract

Background: Acute renal failure (ARF) is still associated with a high incidence of morbidity and mortality, as well as a high risk of developing chronic renal failure. ARF is a major public health problem, it is associated with high mortality, morbidity, and long-term risk of chronic kidney disease. One of the assessments of kidney function is the increase in serum urea in the body. The kidneys have an extraordinary ability to regenerate after injury and fully recover, and clinical options are limited to fluid management and dialysis procedures. The development of new strategies in order to increase the ability of kidney regeneration due to ARF, and to maintain kidney function both in the short term and in the long term is needed. This study aims to determine the effect of low-dose MSC-CM on urea levels in ARF. Method: This research is an in vivo research with the type of research Post Test Only Control Group Design. This study used a model of acute renal failure by inducing gentamicin and used 2 research groups, namely the control group (PBS), and the treatment group (MSC-CM 0.2. urea was examined using a spectrophotometer and then analyzed by unpaired t-test. Result: The results of this study showed that the mean urea levels between the control group (19.46 ± 0.56 mg / dL) and the treatment group (13.96 ± 0.73 mg / dL) were significantly different (p <0.05). Conclusion: The conclusion of this study indicated that there was an effect of low-dose of MSC-CM on urea levels in acute renal failure.
Co-Authors Bhirau Wilaksono Fatimah, Nandiah Istania, Rizqi Windhu Sri Istiqomah, Dyah Ayu Fitri Nur Anna Chalimah Sadyah NURUL HIDAYAH Ramadhanti, Olifiarsy Wiet Rizkiyani, Elytia Mutia