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Effect of Starfruit Extract (Averrhoa bilimbi Linn) Using a Combination of Water, Acetone, and Methanol Solvents on Phenolic Content and Antioxidant Capacity Putri, Dita Audia; Gultom, Milanda Fiorella; Binukajati, Farras Agung; Maulana, Asy Syifa Tickna; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.343

Starfruit leaves, which belong to the Averrhoa family and contain tannin, saponin, flavonoid, and terpenoid compounds, have been widely used in traditional medicine due to their pharmacological benefits. This study aims to investigate the effect of solvent maceration on the extraction of total phenolics from starfruit leaves and to evaluate their antioxidant activity capacity. The study utilized Wuluh starf leaves obtained from Biofarmaka IPB University, Bogor, Indonesia, in February 023. The total phenolic content (TPC) and antioxidant tests, such as ABTS and FRAP, were influenced by the maceration stage using solvents, such as a combination of water, ethanol, and methanol. The Folin-Ciocalteu reagent and sodium carbonate were used to determine the total phenolic content, and antioxidant tests, such as the reaction with radical cations (ABTS) and the antioxidant reaction with reduction of ferri-trpyridytriazine (FRAP), were used to evaluate the antioxidant activity capacity. The results indicate that extraction with 100% acetone solvent has the FRAP method's highest phenolic content and antioxidant capacity, with 10.6325 mg GAE/g DW and 34.26641 ± 1.82 µmol TE/g DW, respectively. On the other hand, the 50:50 ethanol:acetone solvent had the highest antioxidant activity using the ABTS method, with 100.31 ± 4.80 µmol TE/g DW. The TPC-FRAP standard curve shows a positive correlation with R2 = 0.9388, while the TPC-ABTS and FRAP-ABTS standard curves do not significant correlation with the R2 values of -0.09045 and 0.08038, respectively.

Optimization of Extraction Solvent for Analysis of Total Phenolic and Antioxidant Activity of Guava Leaves (Psidium guajava) using Simplex Centroid Design Damarhati, Lelly Aulia; Fajriansyah, Muhamad Renza; Anyalirria; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.344

Guava (Psidium guajava) is a popular tropical plant widely used as processed food and medicine for various diseases. This plant contains highly bioactive compounds like quercetin, flavonoids, and ferulic acid. The bioactive content can be influenced by the extraction method. However, the solvent used for extraction can also affect the bioactive content. Therefore, this study aims to determine the best solvent or combination of extraction solvents to extract guava leaves based on total phenolic content (TPC) and antioxidant activity using simplex centroid design (SCD). This research used guava leaf samples with various solvent combinations of water, ethanol, and acetone. The determination of phenolic content was determined using the TPC method, while the determination of antioxidants was conducted using the FRAP and ABTS methods. The research results showed that the highest TPC levels were produced by the solvent water : ethanol : acetone. Then the highest levels of FRAP and ABTS antioxidants were produced by the water: ethanol solvent. The solvent optimization solution obtained was using water: ethanol: acetone with a ratio of 0.462:0.436:0.102, respectively.

Effect of Starfruit Extract (Averrhoa bilimbi Linn) Using a Combination of Water, Acetone, and Methanol Solvents on Phenolic Content and Antioxidant Capacity Putri, Dita Audia; Gultom, Milanda Fiorella; Binukajati, Farras Agung; Maulana, Asy Syifa Tickna; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.343

Starfruit leaves, which belong to the Averrhoa family and contain tannin, saponin, flavonoid, and terpenoid compounds, have been widely used in traditional medicine due to their pharmacological benefits. This study aims to investigate the effect of solvent maceration on the extraction of total phenolics from starfruit leaves and to evaluate their antioxidant activity capacity. The study utilized Wuluh starf leaves obtained from Biofarmaka IPB University, Bogor, Indonesia, in February 023. The total phenolic content (TPC) and antioxidant tests, such as ABTS and FRAP, were influenced by the maceration stage using solvents, such as a combination of water, ethanol, and methanol. The Folin-Ciocalteu reagent and sodium carbonate were used to determine the total phenolic content, and antioxidant tests, such as the reaction with radical cations (ABTS) and the antioxidant reaction with reduction of ferri-trpyridytriazine (FRAP), were used to evaluate the antioxidant activity capacity. The results indicate that extraction with 100% acetone solvent has the FRAP method's highest phenolic content and antioxidant capacity, with 10.6325 mg GAE/g DW and 34.26641 ± 1.82 µmol TE/g DW, respectively. On the other hand, the 50:50 ethanol:acetone solvent had the highest antioxidant activity using the ABTS method, with 100.31 ± 4.80 µmol TE/g DW. The TPC-FRAP standard curve shows a positive correlation with R2 = 0.9388, while the TPC-ABTS and FRAP-ABTS standard curves do not significant correlation with the R2 values of -0.09045 and 0.08038, respectively.

Optimization of Extraction Solvent for Analysis of Total Phenolic and Antioxidant Activity of Guava Leaves (Psidium guajava) using Simplex Centroid Design Damarhati, Lelly Aulia; Fajriansyah, Muhamad Renza; Anyalirria; Putra, Raihan Permana; Liwanda, Novian; Nurcholis, Waras
Jurnal Jamu Indonesia Vol. 8 No. 3 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i3.344

Guava (Psidium guajava) is a popular tropical plant widely used as processed food and medicine for various diseases. This plant contains highly bioactive compounds like quercetin, flavonoids, and ferulic acid. The bioactive content can be influenced by the extraction method. However, the solvent used for extraction can also affect the bioactive content. Therefore, this study aims to determine the best solvent or combination of extraction solvents to extract guava leaves based on total phenolic content (TPC) and antioxidant activity using simplex centroid design (SCD). This research used guava leaf samples with various solvent combinations of water, ethanol, and acetone. The determination of phenolic content was determined using the TPC method, while the determination of antioxidants was conducted using the FRAP and ABTS methods. The research results showed that the highest TPC levels were produced by the solvent water : ethanol : acetone. Then the highest levels of FRAP and ABTS antioxidants were produced by the water: ethanol solvent. The solvent optimization solution obtained was using water: ethanol: acetone with a ratio of 0.462:0.436:0.102, respectively.

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