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All Journal Majalah Obat Tradisional Sciences of Phytochemistry
Hauwa, Alkasim Yahaya
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Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Medicine & Pharmacology Public Health

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An In Vitro Assessment of the Antioxidant Activity of Detarium microcarpum Guill. & Perr. Fabaceae Dahiru, Mubarak Muhammad; Oni, Abdulhasib Oluwatobi; Danga, James; Alhaji, Aisha Alfa; Jonah, Faith; Hauwa, Alkasim Yahaya; Muhammad, Zainab
Sciences of Phytochemistry Volume 3 Issue 2
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphy0302267

Abstract

Medicinal plants are regarded as important sources of exogenous antioxidants due to their phytoconstituents’ free radical scavenging potential. The present study explores the phytoconstituents and antioxidant activity of n-hexane (HFDM) and chloroform (CFDM) fractions of Detarium microcarpum for potential use in the phytotherapy of oxidative stress-linked ailments. The phytoconstituents were qualitatively determined, while the antioxidant activity was determined by in vitro assays. Alkaloids, saponins, steroids, and flavonoids were detected in both fractions, while glycosides and terpenoids were absent. The HFDM (55.40 ± 2.66 AAE µg/mL) showed a significantly higher total antioxidant capacity than the CFDM (34.84 ± 1.22 AAE µg/mL, p<0.05) at the tested concentration (100 µg/mL) while the CFDM (57.84 ± 2.16 AAE µg/mL) exhibited a significantly higher ferric reducing antioxidant power than the HFDM (46.11 ± 1.91 AAE µg/mL, p<0.05) at the tested concentration (100 µg/mL). In the ferric thiocyanate assay, there was no significant (p>0.05) difference between the HFDM (65.59 ± 2.32%) and CFDM (77.42 ± 2.95%). However, both fractions exhibited significantly higher percentage inhibition than ascorbic acid (9.68 ± 5.41%, p<0.05). Moreover, the HFDM (0.21 ± 0.01 nmol/mL) exhibited a significantly lower MDA concentration than the CFDM (0.66 ± 0.06 nmol/mL) and AA (0.46 ± 0.02 nmol/mL). Additionally, ascorbic acid (0.46 ± 0.02 nmol/mL) showed a significantly lower MDA concentration than CFDM (0.66 ± 0.06 nmol/mL). The n-hexane and chloroform fractions of the plants showed promising antioxidant potential, which might be attributed to the identified phytochemicals that have potential applications in the phytotherapy of oxidative stress-linked diseases.
Fourier-transform Infrared (FTIR) Characterization and Radical Scavenging Activity Ziziphus spina-christi (Rhamnaceae) Rootbark Fractions Dahiru, Mubarak Muhammad; Hauwa, Alkasim Yahaya; Zainab, Muhammad; Pius, Pwadumadi; Yakubu, Nuhu Dangana
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95278

Abstract

This study focused on the secondary metabolite characterization and radical scavenging activity of the chloroform (CF), ethyl acetate (EF), and aqueous fractions (AF) of Ziziphus spina-christi to ascertain its therapeutic potential against oxidative stress. Fourier-transform infrared (FTIR) characterization and determination of in vitro radical scavenging activity of the plant were carried out. Alkaloids, saponins, and flavonoids were present in all the fractions with steroids absent in the AF. The FTIR characterization detected alcohol, conjugated alkenes, and amine groups in the CF and EF. However, alkanes, aromatic amines, sulfonates, and monosubstituted alkanes were also detected in the latter. Moreover, carboxylic acid, alkane, alkene, amines, and phenols were identified in the AF. The EF (72.46 ± 0.55 µg/ml AAE) and AF (71.51± 0.46 µg/ml AAE) demonstrated a significantly (p < 0.05) higher total antioxidant capacity (TAC) than CF (50.33 ±0.27 µg/ml AAE). The AF (54.07 ± 0.97 µg/ml AAE) exhibited a significantly (p < 0.05) higher total reducing power (TRP) than the EF (42.76 ± 1.60 µg/ml AAE) and CF (30.13 ± 1.32 µg/ml AAE). A significantly (p < 0.05) higher percentage of lipid peroxidation inhibition was exhibited by the CF (71.25% ±3.41) compared to the EF (54.17% ±2.66). Moreover, all the fractions showed significantly (p < 0.05) higher inhibition than ascorbic acid (18.33% ±1.56). The CF (0.16 ±0.01 nmol/ml) and EF (0.21 ±0.01 nmol/ml) demonstrated a significantly (p < 0.05) lower MDA concentration than the AF (0.42 ±0.01 nmol/ml) and ascorbic acid (0.38 ±0.02 nmol/ml). Conclusively, the Z. spina rootbark has potential antioxidant application in oxidative stress therapy with a focus on anti-lipid peroxidation for the CF though the AF has better TAC and TRP.
Co-Authors Alhaji, Aisha Alfa Dahiru, Mubarak Muhammad Danga, James Jonah, Faith Muhammad, Zainab Oni, Abdulhasib Oluwatobi Pius, Pwadumadi Yakubu, Nuhu Dangana Zainab, Muhammad