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All Journal Chemical Engineering Journal Storage (CEJS) Journal of Biobased Chemicals (JOBC) InSTEM
Afred, Merymistika Yufrani
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Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Chemistry Mathematics Physics Other

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Ekstraksi Senyawa Bioaktif Kulit Kopi dengan Pelarut Aseton Menggunakan Metode Microwave Assisted Extraction dan Analisis Senyawa Fenoliknya Julieta, Theressa; Helda Wika Amini; Ansori Ansori; Yukti Nurani; Ratri Sekaringgalih; Sonya Hakim Raharjo; Merymistika Yufrani Afred
Journal of Biobased Chemicals Vol. 4 No. 2 (2024): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jobc.v4i2.1357

Abstract

Dewasa ini, pemanfaatan kulit buah kopi hanya terbatas sebagai pakan ternak dan pupuk. Maka dari itu, dilakukan penelitian dengan memanfaatkan limbah kulit kopi robusta sebagai bahan baku penelitian. Tujuan dilakukannya penelitian ini antara lain untuk mengetahui pengaruh dari beberapa variabel dan mengetahui kondisi optimum dalam proses ekstraksi senyawa bioaktif limbah kulit kopi. Limbah kulit kopi akan diekstrak menggunakan metode Microwave-Assisted Extraction (MAE) dengan pelarut aseton, dengan beberapa variabel yaitu rasio bahan terhadap pelarut, daya microwave, dan waktu ekstraksi. Analisis identifikasi adanya kandungan senyawa fenolik kemudian juga dilakukan menggunakan spektrofotometri UV-Vis. Variabel rasio bahan, waktu ekstraksi, dan daya microwave terbukti saling berkaitan, sehingga dapat menghasilkan total fenol pada kondisi optimum. Kondisi optimum dalam proses ekstraksi senyawa bioaktif limbah kulit kopi diperoleh pada variabel rasio bahan 0,04 g/ml ; waktu 9 menit ; dan daya 300 watt, dengan hasil total fenol sebesar 8,65 GAE/g sampel.
Pemisahan Surfaktan Anionik dari Air Limbah Deterjen Menggunakan Biokogulan Biji Kelor ( Moringa Oliefera L.) Afred, Merymistika Yufrani Afred; Bibiana Dho Tawa; Pius Dore Ola
Chemical Engineering Journal Storage (CEJS) Vol. 5 No. 4 (2025): Chemical Engineering Journal Storage (CEJS)-Agustus 2025
Publisher : LPPM Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/cejs.v5i04.22824

Abstract

Air limbah deterjen umumnya mengandung senyawa aktif surfaktan anionik. Pembuangan limbah ini langsung ke lingkungan dapat mengganggu biota perairan dan menurunan kualitas tanah serta memiliki efek karsinogenik jika manusia terpapar dalam jangka waktu yang lama. Tujuan penelitian ini untuk menentukan dosis biokoagulan Moringa Oliefera L. (MO) yang efektif untuk menurunkan kadar surfaktan (LAS) pada air limbah deterjen dan mengetahui pengaruh jenis air (air koagulasi, air limbah deterjen dan air keran) terhadap perkecambahan Vigna Radiata.  Hasil analisis diperoleh dosis optimum dengan penambahan biokoagulan biji kelor 2000 mg/L dengan persentase penyisihan LAS sebesar 96%. Selain itu, uji pengaruh jenis air terhadap perkecambahan  kacang hijau diperoleh tinggi batang dan luas daun yang disiram air hasil koagulasi (25,9 cm  dan 14,1 cm2) lebih baik dibandingkan dengan air keran (24,3 cm dan 10,99 cm2) dan air deterjen (24,6 cm dan 9,6 cm2).  Namun  hasil uji Anova menunjukkan dampak konsentrasi LAS pada air tidak ada perbedaan nyata. Kata Kunci: koagulan, biji kelor, air limbah deterjen, LAS   ABSTRACT Removal of Surfactant Anionik From Detergent Wastewater By Biocoagulant Moringa Oleifera Seeds Detergent wastewater commonly have surfaktan anionic as active agent. Disposal of this wastewater directly into the environment can disrupt aquatic biota and reduce soil quality as well as have carcinogenic effects if humans are exposed to it for a long period of time. The aim of this research is determined optimum dosages Moringa oleifera L. (MO) as biocoagulant to remove LAS from laundry wastewater and evaluate the effect of coagulation water, detergent wastewater and tap water to Vigna radiata germination. The result show that optimum dosages of Moringa oleifera L. is 2000 mg/L which could remove 96% of LAS in sample. Moreover, the study of the influence water to Vigna radiata germination show the lenght of steam and leaf by coagulation water ((25,9 cm and 14,1 cm2) better than coagulation water (24,3 cm and 10,99 cm2) and tap water (24,6 cm and 9,6 cm2). However, the result anova test show that the effect of LAS concentration in water is not significant.   Keyword: coagulant, Moringa oleifera, detergent wastewater, LAS    
Enhancing Biotechnology Literacy through Kombucha Tea Making Socialization at SMA Negeri 1 Giri Istiqomah Rahmawati; Bekti Palupi; Merymistika Yufrani Afred; Agus Wedi Pratama
Jurnal Inovasi Sains dan Teknologi Untuk Masyarakat Vol. 3 No. 2 (2025): November
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i2.4

Abstract

Biotechnology learning at the secondary school level is often dominated by theoretical approaches, potentially limiting students' understanding of real-life scientific applications. This community service activity aimed to implement the Tri Dharma of Higher Education through socialization and training on kombucha tea making at SMA Negeri 1 Giri, Banyuwangi. The primary objective of this program was to strengthen students' applied biotechnology literacy and foster an entrepreneurial spirit by introducing the business potential of fermented products. The implementation method employed a participatory approach involving 53 students as active participants on March 18, 2025. The activity stages included interactive material delivery regarding SCOBY (Symbiotic Culture of Bacteria and Yeast) microbiology and the health benefits of kombucha, followed by practical demonstrations covering the process from starter inoculation to fermentation. In addition to technical aspects, this activity specifically incorporated training on simple economic analysis, including calculations for raw material costs, production expenses, and the determination of competitive selling prices for a home-industry scale. The results indicated high participant enthusiasm, evidenced by active participation in discussion and quiz sessions. Students successfully gained a deep understanding of the biochemical reactions occurring during fermentation and the potential of kombucha as a functional beverage. Furthermore, the provided business insights transformed students' perspectives, highlighting the high economic value of biotechnology products. It is concluded that the integration of practical training and business insights effectively enhances students' scientific competence while supporting the implementation of the entrepreneurship theme within the Merdeka Curriculum.
Pengaruh Variasi Pati Sukun (Artocarpus Altilis) dan Gliserol terhadap Karakteristik Edible Film Fibarzi, Wiza Ulfa; Rizka Nurlaila; Andira, Riza; Hasfita, Fikri; Sulhatun; Afred, Merymistika Yufrani
Chemical Engineering Journal Storage (CEJS) Vol. 6 No. 02 (2026): Chemical Engineering Journal Storage (CEJS)-April 2026
Publisher : LPPM Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/cejs.v6i02.26798

Abstract

Peningkatan limbah plastik yang sulit terdegradasi menjadi permasalahan serius bagi lingkungan, khususnya dalam sektor pengemasan pangan. Oleh karena itu, diperlukan alternatif kemasan ramah lingkungan yang bersifat biodegradable, seperti edible film berbasis pati. Meskipun pemanfaatan pati dan plasticizer telah banyak diteliti, kajian mengenai pengaruh kombinasi pati sukun (Artocarpus altilis) dengan plasticizer campuran serta keterkaitannya terhadap sifat mekanik dan biodegradabilitas masih terbatas. Penelitian ini bertujuan untuk menganalisis pengaruh variasi konsentrasi pati sukun (3, 4, 5, dan 6 gr) dan gliserol (2, 3, 4 dan 5 ml) dengan penambahan sorbitol terhadap karakteristik edible film. Karakterisasi meliputi kadar air, swelling, kuat tarik, elongasi, biodegradabilitas, serta analisis gugus fungsi menggunakan FTIR. Hasil penelitian menunjukkan bahwa kadar air pati sebesar 11,92% telah memenuhi standar. Nilai kuat tarik berkisar antara 0,80–1,11 MPa, sedangkan elongasi 7,44–9,58%. Peningkatan konsentrasi gliserol menurunkan kuat tarik namun meningkatkan fleksibilitas film. Nilai swelling terendah sebesar 11,26% diperoleh pada formulasi 3 gram pati dan 2 mL gliserol. Seluruh sampel menunjukkan biodegradabilitas yang sangat baik dengan degradasi 100% dalam waktu kurang dari satu minggu. Analisis FTIR menunjukkan tidak terbentuknya gugus fungsi baru, sehingga interaksi yang terjadi bersifat fisik. Hasil ini menunjukkan bahwa kombinasi pati sukun dan plasticizer campuran berpotensi menghasilkan edible film dengan biodegradabilitas tinggi, meskipun diperlukan pengembangan lebih lanjut untuk meningkatkan sifat mekaniknya agar memenuhi standar yang berlaku.
Extraction of Bioactive Compounds from Coffee Husk with Acetone Using Microwave Assisted Extraction Method and Analysis of Phenolic Compounds Theressa Julieta Putri Andina; Helda Wika Amini; Ansori Ansori; Yukti Nurani; Ratri Sekaringgalih; Sonya Hakim Raharjo; Merymistika Yufrani Afred
JOBC Vol. 4 No. 2 (2024): Journal of Biobased Chemicals
Publisher : University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

. Nowadays, coffee husks are limited to animal feed and fertilizer. Therefore, a study used Robusta coffee husk waste as a raw material. The purpose of this study was to determine the effect of several variables and to determine the optimum conditions in the process of extracting bioactive compounds from coffee husk waste. The coffee husk contains bioactive compounds, including anthocyanins and polyphenol compounds, such as flavonols, flavan-3-ols, hydroxycinnamic acids, and caffeine. Coffee husk waste will be extracted using the Microwave-Assisted Extraction (MAE) method with acetone solvent, with several variables: the ratio of material to solvent, microwave power, and extraction time. Analysis of the identification of phenolic compound content was then also carried out using UV-Vis spectrophotometry. The variables of material ratio, extraction time, and microwave power were proven to be interrelated so that they could produce total phenol at optimum conditions. The optimum conditions for extracting bioactive compounds from coffee husk waste were obtained at a material ratio of 0.04 g/mL, 9 minutes, and power of 300 watts, with a total phenol yield of 8.65 GAE/g sample.
Co-Authors Agus Wedi Pratama Andira, Riza ansori ansori Ansori Ansori Bekti Palupi Bibiana D. Tawa Fibarzi, Wiza Ulfa Helda Wika Amini Istiqomah Rahmawati Julieta, Theressa Pius Dore Ola Ratri Sekaringgalih Rizka Nurlaila Sonya Hakim Raharjo Sulhatun Theressa Julieta Putri Andina Yukti Nurani