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All Journal JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Simbolon, Ronal
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology

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Isolation of Flavonoid Compound from Kareumbi Fruit (Homalanthus populneus (Geiseler) Pax) Marpaung, Lamek; Simbolon, Ronal
Journal of Chemical Natural Resources Vol. 6 No. 2 (2024): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v6i2.18120

Abstract

Flavonoid compounds were isolated from Kareumbi fruit (Homalanthus populneus) using a sequential extraction and purification process. Methanol-concentrated extract was partitioned with ethyl acetate and n-hexane. The concentrated methanol extract was separated by column chromatography using a gradient elution of chloroform and methanol, varying the ratio 90:10; 80:20; 70:30; and 60:40 (v/v). Subsequent column chromatography yielded a yellowish-orange paste (19.6 mg, Rf = 0.73) from the chloroform:methanol (70:30) fraction. UV-visible, FT-IR, and 1H-NMR spectroscopic analysis confirmed that the isolated compounds belonged to the flavonol class of flavonoids.
Co-Authors Lamek Marpaung, Lamek