Diversity of ecosystem component in rice agroecosystem is one of the success keys on rice cultivation. Information about the functional diversity of nematodes in rice agroecosystems were still limited, therefore this research aimed to examine the functional diversity of nematodes on rice agroecosystems. This research was carried out by taking samples at rice planting centers in Banyumas, comprises of Wangon, Jatilawang, Ajibarang, Rawalo, and Baturraden. Sampling was carried out randomly by taking soil samples around the rice plants at each of two locations for each sub-district. A total of 100 g of soil was extracted and isolated using the White-head tray method. The observed variables were morphological character and function of nematode genera. Diversity data were analyzed using the Shannon-Weiner diversity index to obtain diversity (H'), evenness (E), and dominance (D) indices. Results exhibited five different functions of nematode comprised of omnivore-predator (Eudorylaimus), omnivore (Dorylaimus), microbial feeder and predator (Diplogaster), bacterivore (Cephalobus and Rhabditis), and herbivore (Hirschmanniella, Meloidogyne, Helicotylenchus). The percentage of genera abundance in rice agroecosystem in Banyumas were Eudorylaimus (32%), Hirschmanniella (23%), Dorylaimus (17%), Meloidogyne 12%, Helicotylenchus (8%), Diplogaster (4%), and Rhabditis and Cephalobus (2%) for each. H' index was ranged from low to moderate, while the E index categorized as moderate to high, and D depicted that some locations were experienced dominance. In conclusion, diversity of nematode functions on rice agroecosystem in Banyumas was greatly diverse, but R1 location in Rawalo district should take a concerned due to the dominance of Meloidogyne.